Identification of a microRNA signature in endothelial cells with mechanical stretch stimulation

Abstract

The current study aimed to verify an miRNA signature in endothelial cells undergoing mechanical stretch stimulation. In the present study, microarray profiling was conducted in order to identify the differential expression of miRNAs in endothelial cells undergoing mechanical stimulation, compared with unstimulated endothelial cells. The microarray data was then validated by reverse transcription‑quantitative polymerase chain reaction. Genes and signaling pathways regulated by the miRNAs were investigated in silico using Gene Ontology and the Kyoto Encyclopedia of Genes or Genomes, which are ontological and network‑mapping algorithms. The microarray data collected demonstrated that 38 miRNAs exhibited significant differential expression in endothelial cells with mechanical stretch stimulation. Of these, 20 were upregulated and 18 were downregulated. The results from the in silico analysis indicated that the miRNAs identified were participants in mechanical stretch‑induced endothelial dysfunction. During the initial stage of vein graft failure, which is induced by endothelial dysfunction, a unique miRNA signature was identified. The identified miRNAs are suggested to be involved in the pathological processes of traumatic injury.

MicroRNA expression profile is altered in the upper airway skeletal muscle tissue of patients with obstructive sleep apnea-hypopnea syndrome

“…Altered expression of miRNAs has been reported to occur on exposure to hypoxia, 23 mechanical stimulation, 24 and inflammation, 25 among other factors. The altered miRNA expression profile in turn may affect these processes.…”

Integrated Small RNA Sequencing, Transcriptome and GWAS Data Reveal microRNA Regulation in Response to Milk Protein Traits in Chinese Holstein Cattle

“…The statistical power of this experimental design was estimated using the SSPA R package ( Iterson et al, 2013 ), which reached 0.63 and 0.76 for the HP vs LP and HD vs. LD, respectively ( Supplementary Figure S1 ). MiRNAs with a p -value <0.05 and log 2 (fold change)| >0.8 were assigned as DE ( Zheng et al, 2015 ). The expression patterns of DE miRNAs across four groups were performed using the k-mean method ( Ahmad and Dey, 2007 ).…”

Mechanisms of Mechanical Force Induced Pulmonary Vascular Endothelial Hyperpermeability

“…miRNAs have been found to be critical modulators of endothelial homeostasis and link to vascular diseases ( Fernandez-Hernando and Suarez, 2018 ). miRNAs in ECs are mechanosensitive that response to mechanical stretch stimulation and exhibited obvious differential expression ( Kumar et al, 2014 ; Zheng et al, 2015 ). After 1h of mechanical ventilation with a tidal volume of 40ml/kg, the expression of 50 miRNAs increased more than 2-fold, and the expression of 15 miRNAs decreased by more than half ( Vaporidi et al, 2012 ).…”