Identification of a Metastasis-Related Protein IFI16 in Esophageal Cancer using a Proteomic Approach

Wang, Zhiyu; Bing, Sheng; Wei, Zichao; Li, Yang; Liu, Zhihua

doi:10.7150/jca.59286

Cited by 2 publications

(1 citation statement)

References 24 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative proteomics has been widely used in oncology to identify biomarkers with diagnostic and therapeutic potential (Wang et al, 2018;Wang et al, 2022). Isobaric tags for relative and absolute quantitation (iTRAQ), a new protein quantitation technique, has the advantages of a simpler labeling process, higher sensitivity, and a wider application range compared with traditional quantitative methods.…”

Section: Introductionmentioning

confidence: 99%

Changes in intracellular and extracellular proteins after ERGIC3 knockdown in lung cancer: Proteins interacting with ERGIC3, HORN, and FLNA

ZHAI,

ZHENG,

LIU

et al. 2023

Biocell

View full text Add to dashboard Cite

Objective: Endoplasmic reticulum-Golgi intermediate compartment 3 (ERGIC3) promotes cell proliferation and metastasis in lung cancer, but its molecular mechanism is unclear. Methods: The GLC-82 cells were randomly divided into the ERGIC3i group and the negative control group. The cells were transfected with ERGIC3 siRNA or control siRNA in the groups, respectively. The ERGIC3-interacting proteins expressed in cells or extracellularly were isolated by the immunoprecipitation method and detected by isobaric tags for relative and absolute quantitation and liquid chromatography-tandem mass spectrometry. The differentially expressed proteins were determined by bioinformatic methods. Results: After ERGIC3 knockdown, 88 extracellular differentially expressed proteins, 41 upregulated and 47 down-regulated, were detected in the supernatant of cultured cells. Among 52 intracellular differentially expressed proteins, 33 were up-regulated and 19 down-regulated. Cluster analysis showed that the extracellular differential proteins are mainly involved in Ca 2+ binding and transport and I-kappa B kinase/NF-kappa B signal transduction. The upregulated proteins are mainly involved in the biological process of H3-K27 and H3-K4 methylation in cells. Co-immunoprecipitation assay showed that proteins interacting with ERGIC3 were rich in cytoskeleton construction and RHO GTPases activated p21-activated kinases. The intersection of these two research methods shows that ERGIC3 interacts with HORN and filamin A (FLNA). Conclusion: Proteomic analyses reveal that ERGIC3 acts as a vesicle transmembrane protein on the distribution of various extracellular and intracellular proteins and regulates the extracellular and intracellular biological processes by specifically binding hornin (HORN) and FLNA proteins. These findings maybe provide new methods and ideas for ERGIC3 as a therapeutic target for lung cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Changes in intracellular and extracellular proteins after ERGIC3 knockdown in lung cancer: Proteins interacting with ERGIC3, HORN, and FLNA

ZHAI,

ZHENG,

LIU

et al. 2023

Biocell

View full text Add to dashboard Cite

show abstract

IFI16 Induced by Direct Interaction between Esophageal Squamous Cell Carcinomas and Macrophages Promotes Tumor Progression via Secretion of IL-1α

Azumi,

Koma,

Tsukamoto

et al. 2023

Cells

View full text Add to dashboard Cite

Tumor-associated macrophages (TAMs), one of the major components of the tumor microenvironment, contribute to the progression of esophageal squamous cell carcinoma (ESCC). We previously established a direct co-culture system of human ESCC cells and macrophages and reported the promotion of malignant phenotypes, such as survival, growth, and migration, in ESCC cells. These findings suggested that direct interactions between cancer cells and macrophages contribute to the malignancy of ESCC, but its underlying mechanisms remain unclear. In this study, we compared the expression levels of the interferon-induced genes between mono- and co-cultured ESCC cells using a cDNA microarray and found that interferon-inducible protein 16 (IFI16) was most significantly upregulated in co-cultured ESCC cells. IFI16 knockdown suppressed malignant phenotypes and also decreased the secretion of interleukin-1α (IL-1α) from ESCC cells. Additionally, recombinant IL-1α enhanced malignant phenotypes of ESCC cells through the Erk and NF-κB signaling. Immunohistochemistry revealed that high IFI16 expression in human ESCC tissues tended to be associated with disease-free survival and was significantly associated with tumor depth, lymph node metastasis, and macrophage infiltration. The results of this study reveal that IFI16 is involved in ESCC progression via IL-1α and imply the potential of IFI16 as a novel prognostic factor for ESCC.

show abstract

Identification of a Metastasis-Related Protein IFI16 in Esophageal Cancer using a Proteomic Approach

Cited by 2 publications

References 24 publications

Changes in intracellular and extracellular proteins after ERGIC3 knockdown in lung cancer: Proteins interacting with ERGIC3, HORN, and FLNA

Changes in intracellular and extracellular proteins after ERGIC3 knockdown in lung cancer: Proteins interacting with ERGIC3, HORN, and FLNA

IFI16 Induced by Direct Interaction between Esophageal Squamous Cell Carcinomas and Macrophages Promotes Tumor Progression via Secretion of IL-1α

Contact Info

Product

Resources

About