Identification of a linear epitope within domain I of Duck Tembusu virus envelope protein using a novel neutralizing monoclonal antibody

Gong, Huimin; Fan, Yufang; Zhou, Peng; Li, Yaqian; Hu, Xueying; Jin, Hui; Luo, Rui

doi:10.1016/j.dci.2020.103906

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…Recombinant (scFv) 3B8. In vitro and in vivo anti-DTMUV infection, results provide valuable enlightenment for the development of DTMUV vaccines and therapeutic agents (Hga et al, 2020).…”

Section: Introductionmentioning

confidence: 93%

Identification and genetic evolution of E gene of a duck Tambusu virus isolated from southeast Guizhou Province

Luo

Long

et al. 2023

Preprint

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Since 2010, several strains of Duck Tembusu Virus (DTMUV) have been isolated from China. In autumn 2021, high mortality was observed in southwestern Guizhou Province, China, and a DTMUV strain named GZSS2022 was successfully isolated. After inoculation with SPF chicken embryos of 10 days age, the embryos died 3-5 days after inoculation, and RT-PCR could still detect DTMUV positive after collecting the allantoic fluid of dead chicken embryos and transmitting it for 4 generations. In addition, the GZSS2022 strain was able to replicate in BHK-21 cells and induce cytopathic effects (CPE), and the virus particles were observed in the cytoplasm by transmission electron microscopy. In animal experiments, GZSS2022 strain can replicate stably and pathological examination results showed that the brain of infected ducks was congested and vascular sheath formed around the blood vessels; Liver lymphocyte infiltration, necrosis, liver bleeding. The E gene of this strain was sequenced by PCR and its homology and genetic evolution were analyzed. It was found that the homology of GZSS2022 with the Beijing duck reference strain (MT108699.1) was the highest 99.39%, and the homology of GZSS2022 with the early isolated reference strain (FX2010) was 96.88%. The GZSS2020 strain was located in the same subbranch as the reference strain from Beijing duck in China. The results of this study indicate that a novel highly virulent Tambusu virus has appeared for the first time in Guizhou Province, China, and further lay the foundation for the study of the pathogenicity and host range of this novel Tambusu virus.

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“…Recombinant (scFv) 3B8. In vitro and in vivo anti-DTMUV infection, results provide valuable enlightenment for the development of DTMUV vaccines and therapeutic agents (Hga et al, 2020).…”

Section: Introductionmentioning

confidence: 93%

Identification and genetic evolution of E gene of a duck Tambusu virus isolated from southeast Guizhou Province

Luo

Long

et al. 2023

Preprint

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“…Truncated DTMUV E protein expressed using a rod-shaped virus expression system can induce specific antibodies in ducklings, making it a potential candidate vaccine for preventing duckling infections [117]. Vaccination of ducklings with recombinant rod-shaped viruses containing the DTMUV E gene induces strong humoral and cellular immune responses, providing 100% protective immunity against the DTMUV YY5 strain [50].…”

Section: Vaccinationmentioning

confidence: 99%

Advancements in Research on Duck Tembusu Virus Infections

Cheng,

Wang,

et al. 2024

Viruses

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Duck Tembusu Virus (DTMUV) is a pathogen of the Flaviviridae family that causes infections in poultry, leading to significant economic losses in the duck farming industry in recent years. Ducks infected with this virus exhibit clinical symptoms such as decreased egg production and neurological disorders, along with serious consequences such as ovarian hemorrhage, organ enlargement, and necrosis. Variations in morbidity and mortality rates exist across different age groups of ducks. It is worth noting that DTMUV is not limited to ducks alone; it can also spread to other poultry such as chickens and geese, and antibodies related to DTMUV have even been found in duck farm workers, suggesting a potential risk of zoonotic transmission. This article provides a detailed overview of DTMUV research, delving into its genomic characteristics, vaccines, and the interplay with host immune responses. These in-depth research findings contribute to a more comprehensive understanding of the virus’s transmission mechanism and pathogenic process, offering crucial scientific support for epidemic prevention and control.

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Mapping of a unique epitope on domain III of the envelope protein of Tembusu virus

Wang

Yang

et al. 2021

Virus Research

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Identification of a linear epitope within domain I of Duck Tembusu virus envelope protein using a novel neutralizing monoclonal antibody

Cited by 4 publications

References 24 publications

Identification and genetic evolution of E gene of a duck Tambusu virus isolated from southeast Guizhou Province

Identification and genetic evolution of E gene of a duck Tambusu virus isolated from southeast Guizhou Province

Advancements in Research on Duck Tembusu Virus Infections

Mapping of a unique epitope on domain III of the envelope protein of Tembusu virus

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