Identification of a human monoclonal Fab with neutralizing activity against H3N2 influenza A strain from a newly constructed human Fab library

Shibuya, Tomoyoshi; Yamashiro, Tetsu; Masaike, Yuka; Ohuchi, Masanobu; Uechi, Gen-ichiro; Nishizono, Akira

doi:10.1111/j.1348-0421.2008.00025.x

Cited by 4 publications

(2 citation statements)

References 33 publications

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“…One MAb, designated S139/1, was reported to neutralize not only H3 viruses but also H1, H2, and H13 viruses through binding of antigenic site B (52). Human H3 MAbs have been created using phage display (23,24,29), plasmablast single cell cloning (46), or hybridoma technology (50,53). Murine MAbs against 1957 H2 also have been made (21,35,39) and used for antigenic mapping (28,35,49), including an antibody directed to an HA stem region epitope that is preserved across influenza virus phylogenetic group 1 HAs (25).…”

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confidence: 99%

Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses

Krause

Tsibane

Tumpey

et al. 2012

J Virol

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Investigation of the human antibody response to the 1957 pandemic H2N2 influenza A virus has been largely limited to serologic studies. We generated five influenza virus hemagglutinin (HA)-reactive human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of healthy donors who were born between 1950 and 1968. Two MAbs reacted with the pandemic H2N2 virus, two recognized the pandemic H3N2 virus, and remarkably, one reacted with both the pandemic H2N2 and H3N2 viruses. Each of these five naturally occurring MAbs displayed hemagglutination inhibition activity, suggesting specificity for the globular head domain of influenza virus HA. When incubated with virus, MAbs 8F8, 8M2, and 2G1 each elicited H2N2 escape mutations immediately adjacent to the receptor-binding domain on the HA globular head in embryonated chicken eggs. All H2N2-specific MAbs were able to inhibit a 2006 swine H2N3 influenza virus. MAbs 8M2 and 2G1 shared the V H 1-69 germ line gene, but these antibodies were otherwise not genetically related. Each antibody was able to protect mice in a lethal H2N2 virus challenge. Thus, even 43 years after circulation of H2N2 viruses, these subjects possessed peripheral blood B cells encoding potent inhibiting antibodies specific for a conserved region on the globular head of the pandemic H2 HA.

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confidence: 99%

Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses

Krause

Tsibane

Tumpey

et al. 2012

J Virol

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“…Several studies have shown that BnAbs targeting conserved HA stem or head domains can be induced upon seasonal influenza infection [ 67 , 92 ], H5N1 infection [ 73 , 95 , 96 ], and pdmH1N1 infection [ 72 , 78 , 101 – 104 ] in humans. Several other reports have also shown that seasonal or pdmH1N1 vaccines can induce such Abs against diverse viruses [ 65 , 68 , 75 , 76 , 81 , 88 ].…”

Section: Induction Of Bnabsmentioning

confidence: 99%

Prospects of HA-Based Universal Influenza Vaccine

Hashem

2015

BioMed Research International

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Current influenza vaccines afford substantial protection in humans by inducing strain-specific neutralizing antibodies (Abs). Most of these Abs target highly variable immunodominant epitopes in the globular domain of the viral hemagglutinin (HA). Therefore, current vaccines may not be able to induce heterosubtypic immunity against the divergent influenza subtypes. The identification of broadly neutralizing Abs (BnAbs) against influenza HA using recent technological advancements in antibody libraries, hybridoma, and isolation of single Ab-secreting plasma cells has increased the interest in developing a universal influenza vaccine as it could provide life-long protection. While these BnAbs can serve as a source for passive immunotherapy, their identification represents an important step towards the design of such a universal vaccine. This review describes the recent advances and approaches used in the development of universal influenza vaccine based on highly conserved HA regions identified by BnAbs.

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Phage-display technology for the production of recombinant monoclonal antibodies

Rajput¹,

Khanna²,

Pradhan³

2014

MATER METHODS

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Identification of a human monoclonal Fab with neutralizing activity against H3N2 influenza A strain from a newly constructed human Fab library

Cited by 4 publications

References 33 publications

Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses

Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses

Prospects of HA-Based Universal Influenza Vaccine

Phage-display technology for the production of recombinant monoclonal antibodies

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