Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells.

Abstract

We have isolated cDNAs representing a previously unrecognized human gene that apparently encodes a protein-tyrosine kinase. We have designated the gene as HCK (hemopoietic cell kinase) because its expression is prominent in the lymphoid and myeloid lineages of hemopoiesis. Expression in granulocytic and monocytic leukemia cells increases after the cells have been induced to differentiate. The 57-kilodalton protein encoded by HCK resembles the product of the proto-oncogene c-src and is therefore likely to be a peripheral membrane protein. HCK is located on human chromosome 20 at bands q11-12, a region that is affected by interstitial deletions in some acute myeloid leukemias and myeloproliferative disorders. Our findings add to the diversity of protein-tyrosine kinases that may serve specialized functions in hemopoietic cells, and they raise the possibility that damage to HCK may contribute to the pathogenesis of some human leukemias.

Structure and expression of c-fgr protooncogene mRNA in Epstein-Barr virus converted cell lines

“…As shown in Figure 4, the N-terminal 75 amino-acids of the predicted cfgr protein have low homology with the N-termini of the predicted human lyn, hck, fyn, c-src and c-yes proteins. In contrast, our data and previously published sequence indicate strong homology between these proteins from amino- Figure 4 Optimal alignment of N-terminal 174 amino-acids of predicted c-fgr protein with predicted N-terminal amino-acid sequences of human lyn , hek (Quintrell et al, 1987), fyn , c-src (Tanaka et al, 1987) and c-yes (Sukegawa et al, 1987) proteins. Percentage homologies with amino-acids 1-75 and 76-529 of the predicted cfgr protein are shown.…”

“…The c-fgr gene is a member of a family which also includes c-src (Tanaka et al, 1987), fyn , hck (Quintrell et al, 1987), Ick (Voronova & Sefton, 1986), tyn , c-tkl (Strebhardt et al, 1987) and c-yes (Sukegawa et al, 1987). Unlike other tyrosine kinases such as c-erb-B and c-fms these proteins do not appear to be cell surface receptors, since they are thought to be located at the cytoplasmic surface of the plasma membrane (Pellman et al, 1985).…”

Gene expression profiling of alpha‐radiation‐induced rat osteosarcomas: Identification of dysregulated genes involved in radiation‐induced tumorigenesis of bone

“…Interestingly, the Src kinase signaling pathway has been shown to be associated with the bone formation and tumorigenic processes, including cell proliferation, adhesion and motility. 28,29 We found that hematopoietic cell kinase (Hck), a member of the Src family of tyrosine kinases, 30 was highly expressed in radiation-induced OS compared with normal OB. Consistent with our data, Akap12, also known as Src-suppressed C-kinase substrate (SSeCKS), a scaffolding protein for protein kinases A and C, has been originally identified as a gene downregulated in response to the Src and Ras activation.…”

Fourier transform-ion cyclotron resonance mass spectrometric resolution, identification, and screening of non-covalent complexes of Hck Src homology 2 domain receptor and ligands from a 324-member peptide combinatorial library

“…The hematopoietic cell kinase (Hck) SH2 domain is a member of the Src SH2 subfamily of cellular tyrosine kinases [17,18]. It is preferentially expressed in myeloid and B-lymphoid cells and is bound to B-cell receptors in unstimulated B cell lineages.…”