Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum

Tomás-Cortázar, Julen; Plaza-Vinuesa, Laura; Rivas, Blanca de las; Lavín, José L.; Barriales, Diego; Abecia, Leticia; Mancheño, José M.; Aransay, Ana M.; Múñoz, Rosario; Anguíta, Juan; Rodríguez, Héctor

doi:10.1186/s12934-018-0880-4

Cited by 17 publications

(20 citation statements)

References 38 publications

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“…However, the absence of signal 400 peptides among the subtype B proteins currently characterized, was not a general feature 401 of these proteins. Most of the similar proteins found in the databases (16 versus 12 402 proteins) possess a clear signal peptide, similar to the one recently characterized TanBFnp 403 tannase (Tomás-Cortázar et al 2018). 404 A multiple alignment of these proteins revealed that most of the amino acid 405 residues conserved on the characterized B subtype tannases, were also conserved in 406 similar proteins found in the databases supporting the existence of two tannase subtypes.…”

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confidence: 63%

“…His tag fusion proteins (Tomás-Cortázar et al 2018). The rhodanine colorimetric assay 524 using methyl gallate as substrate was used to assess tannase activity.…”

Section:  Tanbfnp 519 520mentioning

confidence: 99%

“…The first subgroup (subtype A) includes proteins from Atopobium parvulum DSM 20469 T 174 (TanAAp) (Jiménez et al 2014a), Lactobacillus plantarum ATCC 14917 T (TanALp) 175 (Jiménez et al 2014b), Staphylococcus lugdunensis MTCC 3614 (TanA) (Noguchi et al 176 2007, Chaitanyakumar andAnbalagan 2016), and Streptococcus gallolyticus UCN34 177 (TanASg) (Jiménez et al 2014d). The second subgroup (subtype B) is integrated by 178 proteins from Fusobacterium nucleatum F0401 (TanBFn) (Tomás-Cortázar et al 2018), 179…”

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confidence: 99%

“…As an example, they are proteins shorter than those 379 in the subtype A group, ranging from 470 to 570 amino acid residues long, with a 380 molecular size that ranges from 50 to 60 kDa (Table 2). Generally, they do not possess a 381 signal peptide, except the recently described TanBFn (Tomás-Cortázar et al 2018). 382…”

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confidence: 99%

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Bacterial tannases: classification and biochemical properties

Rivas

Rodríguez

Anguíta

et al. 2018

Appl Microbiol Biotechnol

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Tannin acyl hydrolases, also known as tannases, are a group of enzymes 22 critical for the transformation of tannins. The study of these enzymes, which initially evolved in different organisms to detoxify and/or use these plant metabolites, has 24 nowadays become relevant in microbial enzymology research due to their relevant role in 25 food tannin transformation. 26 Microorganisms, particularly bacteria, are major sources of tannase. Cloning and 27 heterologous expression of bacterial tannase genes and structural studies have been 28 performed in the last few years. However, a systematic compilation of the information 29 related to all recombinant tannases, their classification and characteristics is missing. In 30 this review, we explore the diversity of heterologously produced bacterial tannases, 31 describing their substrate specificity and biochemical characterization. Moreover, a new 32 classification based on sequence similarity analysis is proposed. Finally, putative tannases 33 have been identified in silico for each group of tannases taking advantage of the use of the 34 "tannase" distinctive features previously proposed. 35 36 37 Keywords Tannase • Feruloyl esterase • Esterase • Hydrolysable tannins • Gallic acid 38 39 The majority of industrial enzymes are classified as "hydrolases", and "tannases" 64 belong to this class. Since their discovery more than a century ago, tannases have been 65 used in the food, feed, beverage, pharmaceutical and chemical industries. Among the 66 commercial applications of tannases, the most relevant are the production of gallic acid, 67 instant tea, and coffee-flavored refreshing drinks. Moreover, these enzymes are used for the clarification of beer and fruits juices, the improvement of flavor of grape wine, and the 69 manufacture of animal feeds (Jana et al. 2014). 70 Tannase production can be achieved from microbial, animal and vegetal sources, 71 but microorganisms are commonly used for its commercial production. Microorganisms 72 are the main source of industrial enzymes due to their biochemical diversity and their 73 amenability to genetic modifications. To date, commercially available tannases are mainly 74 produced by the fermentation of fungi, especially the Aspergillus species, from which only 75 crude enzymes are readily produced (Aguilar et al. 2007). Recombinant expression 76 appears to be an appealing alternative to produce pure enzymes, however, the attempt to 77 clone fungal tannase genes has proven difficult and inefficient, probably due to the 78 complexity of fungal enzymes (Aguilar et al. 2007, Hatamoto et al. 1996, Zhong et al. 79 2004). Therefore, bacterial tannases might constitute an adequate alternative to fungal 80 enzymes. Research on bacterial tannases has been primarily focused on the screening of 81 new tannase-producing bacterial strains with the desired properties. The selected tannase-82 producer strains are often employed either directly or using their crude cellular extracts, 83 without enzyme isolation. Surprisingly, little knowledge is...

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confidence: 63%

“…His tag fusion proteins (Tomás-Cortázar et al 2018). The rhodanine colorimetric assay 524 using methyl gallate as substrate was used to assess tannase activity.…”

Section:  Tanbfnp 519 520mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Bacterial tannases: classification and biochemical properties

Rivas

Rodríguez

Anguíta

et al. 2018

Appl Microbiol Biotechnol

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“…Tannases, namely tannin acyl hydrolase (EC 3.1.1.20), hydrolyze ester and depside bonds of hydrolysable tannins to produce glucose and gallic acid (Lekha and Lonsane 1997). Known to be produced also by bacteria (Reveron et al 2017;Tomás Cortázar et al 2018) and plants (Bains et al 2009), fungal tannases are well known for their catalytic versatility.…”

Section: Tannasesmentioning

confidence: 99%

Biotransformation of industrial tannins by filamentous fungi

Prigione

Spina

Tigini

et al. 2018

Appl Microbiol Biotechnol

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Tannins are secondary metabolites widely distributed in the plant kingdom. They act as growth inhibitors towards many microorganisms: upon microbial attack, they are released helping to fight the infection of plant tissues. Extraction of tannins from plants is an active industrial sector with several applications from the beginning of the industrial era. Actually, tannins have many industrial applications in oenology, animal feeding, mining and chemical industry and, in particular, in the tanning industry. But tannins are also considered very recalcitrant pollutants in wastewaters of different origins. The ability to grow on plant substrates rich in tannins and on industrial tannin preparations is traditionally considered peculiar of some species of fungi that have developed mechanisms to tolerate the toxicity of tannins producing a complex enzymatic pattern active in the transformation of these substrates, mainly by hydrolysis and oxidation. Filamentous fungi capable of degrading tannins could have a strong environmental impact as bioremediation agents mostly in the treatment of tanning wastewaters.

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Emerging Horizons for Industrial Applications of Predatory Bacteria

Herencias

Salgado-Briegas²,

Prieto³

2020

The Ecology of Predation at the Microscale

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This chapter reviews the potential of the predatory bacteria Bdellovibrio bacteriovorus, an obligate predator of other gram-negative bacteria as a biotechnological tool. Due to the unique lifestyle and the different applications, predatory bacteria have awakened interest to be developed as a lytic tool. The lack of physiological and metabolic information makes difficult this development. However, in the last years, different approaches have been described in order to understand the physiology, morphology, and metabolism of the predators, as well as the population dynamics of the prey-predator interactions. Besides its potential of "living antibiotic", predatory bacteria have been proposed as a biocontrol agent in the food industry or aquaculture. A recent work using B. bacteriovorus as a biological lytic tool for the recovery of intracellular bioproducts highlighted the potential use of predators in industrial bioprocesses. The bottlenecks of using other Bdellovibrio and like organisms (BALOs) have been also considered and discussed during this chapter.

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Identification of a highly active tannase enzyme from the oral pathogen Fusobacterium nucleatum subsp. polymorphum

Cited by 17 publications

References 38 publications

Bacterial tannases: classification and biochemical properties

Bacterial tannases: classification and biochemical properties

Biotransformation of industrial tannins by filamentous fungi

Emerging Horizons for Industrial Applications of Predatory Bacteria

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