Identification of a genetic determinant responsible for host specificity in <i>Streptococcus thermophilus</i> bacteriophages

Duplessis, Martin; Moineau, Sylvain

doi:10.1046/j.1365-2958.2001.02521.x

Cited by 168 publications

(155 citation statements)

References 44 publications

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“…Interestingly, the three matrices with the most significant modular structures (40,55,64) were determined for a single bacterial species, Streptococcus thermophilus, and its phages. This finding seems robust, because different laboratories performed the studies and the microbes were isolated from three separate continents.…”

Section: Addressing Sample Composition Biases As Potential Drivers Ofmentioning

confidence: 99%

Statistical structure of host–phage interactions

Flores

Meyer

Valverde

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

298

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Interactions between bacteria and the viruses that infect them (i.e., phages) have profound effects on biological processes, but despite their importance, little is known on the general structure of infection and resistance between most phages and bacteria. For example, are bacteria-phage communities characterized by complex patterns of overlapping exploitation networks, do they conform to a more ordered general pattern across all communities, or are they idiosyncratic and hard to predict from one ecosystem to the next? To answer these questions, we collect and present a detailed metaanalysis of 38 laboratory-verified studies of hostphage interactions representing almost 12,000 distinct experimental infection assays across a broad spectrum of taxa, habitat, and mode of selection. In so doing, we present evidence that currently available host-phage infection networks are statistically different from random networks and that they possess a characteristic nested structure. This nested structure is typified by the finding that hard to infect bacteria are infected by generalist phages (and not specialist phages) and that easy to infect bacteria are infected by generalist and specialist phages. Moreover, we find that currently available host-phage infection networks do not typically possess a modular structure. We explore possible underlying mechanisms and significance of the observed nested host-phage interaction structure. In addition, given that most of the available hostphage infection networks examined here are composed of taxa separated by short phylogenetic distances, we propose that the lack of modularity is a scale-dependent effect, and then, we describe experimental studies to test whether modular patterns exist at macroevolutionary scales.bacteriophage | complex networks | ecology | evolution | nestedness

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Section: Addressing Sample Composition Biases As Potential Drivers Ofmentioning

confidence: 99%

Statistical structure of host–phage interactions

Flores

Meyer

Valverde

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

298

356

View full text Add to dashboard Cite

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“…99 Phages of S. thermophilus can alter their host range by exchanging the variable domains of the anti-receptor gene (responsible for recognition of host receptor) which are flanked by conserved collagen-like repeats that serve as target sites for homologous recombination. 100 The invertible G segment in phage Mu DNA controls the host range of the phage and depending on the orientation of the G segment, two types of phage particles are produced which recognize different cell surface receptors. 101 Many prophages carry key virulence factors, as already observed for stx-bearing prophages.…”

Section: Prophages Are the Major Drivers Of Microbial Evolution And Dmentioning

confidence: 99%

Movers and shakers

et al. 2013

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“…Representation of the proteins has been simplified : in particular the existence of some repeated regions in proteins of family III (including collagen-type repeats) is not reported. Note that Duplessis & Moineau (2001) designated VR1 the Va domain of gp1276 in Sfi21 and VR2 the Vg, Vi and Vj domains in streptococcal phage proteins. (b) VS1 and VS2 conserved motifs delimiting V domains.…”

Section: Modular Structural Proteinsmentioning

confidence: 99%

“…In italics, a complete VS2 motif observed just after a truncated motif. Part of the conserved motif reported by Duplessis & Moineau (2001) at the end of collagen-type repeats in the streptococcal phage proteins is boxed (named here CRR). Bottom panel : aa residues preceding some variable domains in streptococcal phage proteins.…”

Section: Modular Structural Proteinsmentioning

confidence: 99%

Sequence analysis of the lactococcal bacteriophage bIL170: insights into structural proteins and HNH endonucleases in dairy phages The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are bIL170, AF009630; bIL120, AY054975; bIL15, AY054976; bIL191, AY054977; bIL77, AY054978.

et al. 2002

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The complete 31 754 bp genome of bIL170, a virulent bacteriophage of Lactococcus lactis belonging to the 936 group, was analysed. Sixty-four ORFs were predicted and the function of 16 of them was assigned by significant homology to proteins in databases. Three putative homing endonucleases of the HNH family were found in the early region. An HNH endonuclease with zinc-binding motif was identified in the late cluster, potentially being part of the same functional module as terminase. Three putative structural proteins were analysed in detail and show interesting features among dairy phages. Notably, gpl12 (putative fibre) and gpl20 (putative baseplate protein) of bIL170 are related by at least one of their domains to a number of multidomain proteins encoded by lactococcal or streptococcal phages. A 110-to 150-aa-long hypervariable domain flanked by two conserved motifs of about 20 aa was identified. The analysis presented here supports the participation of some of these proteins in host-range determination and suggests that specific adsorption to the host may involve a complex multi-component system. Divergences in the genome of phages of the 936 group, that may have important biological properties, were noted. Insertions/deletions of units of one or two ORFs were the main source of divergence in the early clusters of the two entirely sequenced phages, bIL170 and sk1. An exchange of fragments probably affected the regions containing the putative origin of replication. It led to the absence in bIL170 of the direct repeats recognized in sk1 and to the presence of different ORFs in the ori region. Shuffling of protein domains affected the endolysin (putative cell-wall binding part), as well as gpl12 and gpl20.

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Identification of a genetic determinant responsible for host specificity in Streptococcus thermophilus bacteriophages

Cited by 168 publications

References 44 publications

Statistical structure of host–phage interactions

Statistical structure of host–phage interactions

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