Identification of a gene coding for a deglycosylating enzyme in<i>Hypocrea jecorina</i>

Stals, Ingeborg; Samyn, Bart; Sergeant, Kjell; White, Theresa C.; Hoorelbeke, Katleen; Coorevits, An; Devreese, Bart; Claeyssens, Marc; Piens, Kathleen

doi:10.1111/j.1574-6968.2009.01849.x

Cited by 55 publications

(59 citation statements)

References 39 publications

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“…Some members of the chitinases were only identified based on the recent genome analyses. As described more recently, some members of GH family 18 do not exhibit chitinase activity but instead have endo-N-acetlyglucosaminase (ENGase) activity and cleave between two N-acetylglucosamine residues in the carbohydrate antennae of proteins (336). They are therefore involved in protein deglycosylation.…”

Section: Cazymesmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

163

195

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SUMMARYThe genusTrichodermacontains fungi with high relevance for humans, with applications in enzyme production for plant cell wall degradation and use in biocontrol. Here, we provide a broad, comprehensive overview of the genomic content of these species for “hot topic” research aspects, including CAZymes, transport, transcription factors, and development, along with a detailed analysis and annotation of less-studied topics, such as signal transduction, genome integrity, chromatin, photobiology, or lipid, sulfur, and nitrogen metabolism inT. reesei,T. atroviride, andT. virens, and we open up new perspectives to those topics discussed previously. In total, we covered more than 2,000 of the predicted 9,000 to 11,000 genes of eachTrichodermaspecies discussed, which is >20% of the respective gene content. Additionally, we considered available transcriptome data for the annotated genes. Highlights of our analyses include overall carbohydrate cleavage preferences due to the different genomic contents and regulation of the respective genes. We found light regulation of many sulfur metabolic genes. Additionally, a new Golgi 1,2-mannosidase likely involved inN-linked glycosylation was detected, as were indications for the ability ofTrichodermaspp. to generate hybrid galactose-containingN-linked glycans. The genomic inventory of effector proteins revealed numerous compounds unique toTrichoderma, and these warrant further investigation. We found interesting expansions in theTrichodermagenus in several signaling pathways, such as G-protein-coupled receptors, RAS GTPases, and casein kinases. A particularly interesting feature absolutely unique toT. atrovirideis the duplication of the alternative sulfur amino acid synthesis pathway.

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Section: Cazymesmentioning

confidence: 99%

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Schmoll

Dattenböck

Carreras-Villaseñor

et al. 2016

Microbiol Mol Biol Rev

163

195

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“…Group A is predicted to include mainly exochitinases (Gruber & Seidl-Seiboth, 2012), although the A-II subgroup member Cfcl from Aspergillus niger exhibits exo-b-N-acetylglucosaminidase activity (van Munster et al, 2012). Characterized group B chitinases are mostly endo-acting enzymes, while the B-V group contains members with endo-b-N-acetylglucosaminidase (ENGase) activity (Stals et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Identifying glycoside hydrolase family 18 genes in the mycoparasitic fungal species Clonostachys rosea

et al. 2015

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Clonostachys rosea is a mycoparasitic fungal species that is an efficient biocontrol agent against many plant diseases. During mycoparasitic interactions, one of the most crucial steps is the hydrolysis of the prey's fungal cell wall, which mainly consists of glucans, glycoproteins and chitin. Chitinases are hydrolytic enzymes responsible for chitin degradation and it is suggested that they play an important role in fungal-fungal interactions. Fungal chitinases belong exclusively to the glycoside hydrolase (GH) family 18.These GH18 proteins are categorized into three distinct phylogenetic groups (A, B and C), subdivided into several subgroups. In this study, we identified 14 GH18 genes in the C. rosea genome, which is remarkably low compared with the high numbers found in mycoparasitic Trichoderma species. Phylogenetic analysis revealed that C. rosea contains eight genes in group A, two genes in group B, two genes in group C, one gene encoding a putative ENGase (endo-b-N-acetylglucosaminidase) and the ech37 gene, which is of bacterial origin. Gene expression analysis showed that only two genes had higher transcription levels during fungal-fungal interactions, while eight out of 14 GH18 genes were triggered by chitin. Furthermore, deletion of the C group chiC2 gene decreased the growth inhibitory activity of C. rosea culture filtrates against Botrytis cinerea and Rhizoctonia solani, although the biocontrol ability of C. rosea against B. cinerea was not affected. In addition, a potential role of the CHIC2 chitinase in the sporulation process was revealed. These results provide new information about the role of GH18 proteins in mycoparasitic interactions.

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“…Flammulina velutipes Endo FV [137] Hypocrea jecorina (Trichoderma reesei) Endo T [136] A gene encoding tomato ENGase LE was identified and expressed in E. coli in which the substrate specificity remained identical to the native enzyme [121]. Moreover, real-time PCR demonstrated that the gene expression did not vary during ripening and thus that the ENGase LE activity was ubiquitously expressed.…”

Section: Plant N-glycan-engasesmentioning

confidence: 96%

“…However, this de-N-glycosylation activity is widely distributed [137] and several highly homologous proteins or gene products are found among ascomycetes [136]. Both Endo T and Endo FV hydrolyze high-mannose type structures as observed in fungal and yeast glycoproteins, but do not release complex type N-glycans [136,137]. Endo T was shown to be responsible for the microheterogeneity observed for H. jecorina cellulases and hemicellulases [138].…”

Section: Fungal N-glycan-engasesmentioning

confidence: 99%

“…The first biochemically characterized representatives, Endo T from the ascomycete Hypocrea jecorina [136], and Endo FV from the basidiomycete Flammulina velutipes [137] show low sequence homology with the bacterial ENGases and with the fungal chitinases. However, this de-N-glycosylation activity is widely distributed [137] and several highly homologous proteins or gene products are found among ascomycetes [136]. Both Endo T and Endo FV hydrolyze high-mannose type structures as observed in fungal and yeast glycoproteins, but do not release complex type N-glycans [136,137].…”

Section: Fungal N-glycan-engasesmentioning

confidence: 99%

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Endo-N-Acetyl-β-D-Glucosaminidases and Peptide-N4-(N-acetyl-β-D-Glucosaminyl) Asparagine Amidases: More Than Just Tools

Karamanos¹

2013

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Abstract:Since the discovery of endo-N-acetyl-β-D-glucosaminidases (ENGase) and peptide-N4-(N-acetyl-β-D-glucosaminyl) asparagine amidases (PNGase) most of the published work described their use for structural studies. Less attention was given to the potential roles of those enzymes in the physiology of the cells/organisms they produced them. The scope of this review is firstly to analyse the data on the occurrence and characteristics of murein-, chitin-, and N-glycan-ENGases acting on GlcNAc-containing polymers in three structural families, namely murein, chitin, and N-glycosylproteins, and of PNGases, only acting on N-glycosylproteins, and secondly to discuss the biological roles of the enzymes in the producing cells. The analysis demonstrates the remarkable diversity of the enzymes, and simultaneously the interest of studying their substrate specificity and their structural features. Many examples illustrate the importance of the structure/function relationships studies. Diverse biological roles were anticipated, e.g. they are useful for feeding purposes, are implicated in pathogenesis processes, modulate the activity of macromolecules, and help in the destruction of misfolded proteins. Their effect can be direct or indirect, through the reaction products. Current knowledge only partially explains the biological roles of ENGases and PNGases, thus further studies are expected for determining novel possibilities and elucidating other cell pathways.

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Identification of a gene coding for a deglycosylating enzyme inHypocrea jecorina

Cited by 55 publications

References 39 publications

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species

Identifying glycoside hydrolase family 18 genes in the mycoparasitic fungal species Clonostachys rosea

Endo-N-Acetyl-β-D-Glucosaminidases and Peptide-N4-(N-acetyl-β-D-Glucosaminyl) Asparagine Amidases: More Than Just Tools

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