Identification of a GATA motif in the cardiac α-myosin heavy-chain-encoding gene and isolation of a human GATA-4 cDNA

Huang, Weei‐Yuarn; Cukerman, Eva; Liew, Choong‐Chin

doi:10.1016/0378-1119(94)00893-w

Cited by 48 publications

(30 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In cardiogenesis, it is known that GATA4 and Nkx 2.5 are expressed at the early stages during heart development and their expressions occur in a time-dependent manner. GATA4 and Nkx2.5 induce the expression of other genes related with cardiogenic functions, such as those for cell contraction or beating (19)(20)(21)(22)(23). This is consistent with the functional analysis, which showed increased beating in 450-m EBs during the entire culture period and higher expression of cardiogenic markers at early culture time (day 5).…”

Section: Resultssupporting

confidence: 87%

Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11

Hwang

Chung

Ortmann

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

357

359

View full text Add to dashboard Cite

Recently, various approaches for controlling the embryonic stem (ES) cell microenvironment have been developed for regulating cellular fate decisions. It has been reported that the lineage specific differentiation could be affected by the size of ES cell colonies and embryoid bodies (EBs). However, much of the underlying biology has not been well elucidated. In this study, we used microengineered hydrogel microwells to direct ES cell differentiation and determined the role of WNT signaling pathway in directing the differentiation. This was accomplished by forming ES cell aggregates within microwells to form different size EBs. We determined that cardiogenesis was enhanced in larger EBs (450 m in diameter), and in contrast, endothelial cell differentiation was increased in smaller EBs (150 m in diameter). Furthermore, we demonstrated that the EB-size mediated differentiation was driven by differential expression of WNTs, particularly noncanonical WNT pathway, according to EB size. The higher expression of WNT5a in smaller EBs enhanced endothelial cell differentiation. In contrast, the increased expression of WNT11 enhanced cardiogenesis. This was further validated by WNT5a-siRNA transfection assay and the addition of recombinant WNT5a. Our data suggest that EB size could be an important parameter in ES cell fate specification via differential gene expression of members of the noncanonical WNT pathway. Given the size-dependent response of EBs to differentiate to endothelial and cardiac lineages, hydrogel microwell arrays could be useful for directing stem cell fates and studying ES cell differentiation in a controlled manner.hydrogel microwells ͉ stem cell differentiation ͉ WNT signal pathway T he developmental versatility of embryonic stem (ES) cells offers a powerful approach for directing cell fate and is a promising source of progenitors for cell replacement therapy and tissue regeneration (1). ES cells can differentiate into a wide spectrum of cell types, such as cardiomyocytes and endothelial cells, by forming embryoid bodies (EBs) (2). Those lineages arise from distinct mesoderm subpopulations that develop sequentially from premesoderm cells (3). Such lineage specification is highly coordinated with differential changes in gene expression (4-8).Despite the therapeutic potential of ES cells, one of the significant challenges to their widespread clinical use, is the inability to homogeneously direct ES cell differentiation into specific lineages. One reason for the heterogeneity in EB differentiation is caused from variations in EB size (9, 10). To address this challenge for controlling the differentiation of ES cells, various microscale technologies (i.e., surface patterning, hydrogel microwells, and microfluidic systems) have been developed for directing the stem cell fate (11-15). Micropatterning techniques have been used to evaluate the effect of EB size on ES cell differentiation. For instance, microfabricated adhesive stencils were used to pattern ES cells for controlling initial ES cell aggregate sizes...

show abstract

Section: Resultssupporting

confidence: 87%

Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11

Hwang

Chung

Ortmann

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

357

359

View full text Add to dashboard Cite

show abstract

“…Growth tests were performed as described in reference 48 at 37°C using Aspergillus nitrogen-free minimal medium (ANM) with nitrogen sources added at 10 mM (50). The A. nidulans strains used in this study are shown in Table 1 (69), Homo sapiens GATA-1 (P15976) (70,71), H. sapiens GATA-2 (P23769) (72,73), H. sapiens GATA-3 (P23771) (74,75), H. sapiens GATA-4 (P43694) (76), H. sapiens GATA-5 (NP_536721) (77), H. sapiens GATA-6 (Q92908) (78), and mouse GATA-4 (Q08369) (79).…”

Section: A Nidulans Genetic Manipulations and Growth Testsmentioning

confidence: 99%

Multiple Nuclear Localization Signals Mediate Nuclear Localization of the GATA Transcription Factor AreA

et al. 2014

View full text Add to dashboard Cite

“…Therefore, we studied human adenocarcinoma-derived cell lines that are characterized as inducible models for gut cell differentiation. The sequences for the human GATA-4 (22,54) and GATA-6 (49) genes were described previously. These sequences, and a partial sequence deposited in the GenBank database for human GATA-5, were used to design RT-PCR primers to measure in a semiquantitative assay transcripts for human GATA factors in RNA isolated from various cell lines.…”

Section: Factors In Intestinal Epithelium 2903mentioning

confidence: 99%

Distinct Functions Are Implicated for the GATA-4, -5, and -6 Transcription Factors in the Regulation of Intestine Epithelial Cell Differentiation

Gao

Sedgwick

Shi

et al. 1998

Molecular and Cellular Biology

212

191

View full text Add to dashboard Cite

Based on conserved expression patterns, three members of the GATA family of transcriptional regulatory proteins, GATA-4, -5, and -6, are thought to be involved in the regulation of cardiogenesis and gut development. Functions for these factors are known in the heart, but relatively little is understood regarding their possible roles in the regulation of gut-specific gene expression. In this study, we analyze the expression and function of GATA-4, -5, and -6 using three separate but complementary vertebrate systems, and the results support a function for these proteins in regulating the terminal-differentiation program of intestinal epithelial cells. We show that xGATA-4, -5, and -6 can stimulate directly activity of the promoter for the intestinal fatty acidbinding protein (xIFABP) gene, which is a marker for differentiated enterocytes. This is the first direct demonstration of a target for GATA factors in the vertebrate intestinal epithelium. Transactivation by xGATA-4, -5, and -6 is mediated at least in part by a defined proximal IFABP promoter element. The expression patterns for cGATA-4, -5, and -6 are markedly distinct along the proximal-distal villus axis. Transcript levels for cGATA-4 increase along the axis toward the villus tip; likewise, cGATA-5 transcripts are largely restricted to the distal tip containing differentiated cells. In contrast, the pattern of cGATA-6 transcripts is complementary to cGATA-5, with highest levels detected in the region of proliferating progenitor cells. Undifferentiated and proliferating human HT-29 cells express hGATA-6 but not hGATA-4 or hGATA-5. Upon stimulation to differentiate, the transcript levels for hGATA-5 increase, and this occurs prior to increased transcription of the terminal differentiation marker intestinal alkaline phosphatase. At the same time, hGATA-6 steady-state transcript levels decline appreciably. All of the data are consistent with evolutionarily conserved but distinct roles for these factors in regulating the differentiation program of intestinal epithelium. Based on this data, we suggest that GATA-6 might function primarily within the proliferating progenitor population, while GATA-4 and GATA-5 function during differentiation to activate terminal-differentiation genes including IFABP.The intestinal epithelium provides an excellent model system for investigating molecular mechanisms regulating cell lineage establishment, stem cell proliferation, morphogenesis, and the specialization of cell function during terminal differentiation (see references 9 and 16 for reviews). In all vertebrates, the embryonic intestinal lumen is lined by an endoderm-derived epithelial sheet, a monolayer consisting of four principal cell types that are renewed from a proliferating stem cell population. Lineage tracing experiments (7, 39) demonstrated that the four cell types are derived from a small population of multipotential stem cells present near the villus base (the crypt). The differentiating cells migrate from the crypt toward the villus tip, where they eventually ...

show abstract

Identification of a GATA motif in the cardiac α-myosin heavy-chain-encoding gene and isolation of a human GATA-4 cDNA

Cited by 48 publications

References 19 publications

Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11

Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11

Multiple Nuclear Localization Signals Mediate Nuclear Localization of the GATA Transcription Factor AreA

Distinct Functions Are Implicated for the GATA-4, -5, and -6 Transcription Factors in the Regulation of Intestine Epithelial Cell Differentiation

Contact Info

Product

Resources

About