Identification of a functional nuclear export signal in the green fluorescent protein asFP499

Mustafa, Huseyin; Strasser, Bernd; Rauth, Sabine; Irving, Robert A.; Wark, Kim L.

doi:10.1016/j.bbrc.2006.02.077

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…However, the presence of remotely related anthozoan taxa with intense GFP-coloration in low light habitats including the deep sea may argue against a general photoprotective role of these pigments (21,74). Recently, a nuclear export signal and a peroxisomal targeting signal were identified in wildtype forms of asFP499 and eqFP611 (39,114). These signals may also hint at different specific functions of the proteins in the organisms they were isolated from.…”

Section: Fp-induced Cytotoxicitymentioning

confidence: 99%

Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

Wiedenmann¹,

Oswald²,

Nienhaus³

2009

IUBMB Life

218

162

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SummaryThe green fluorescent protein (GFP) from the jellyfish Aequorea victoria can be used as a genetically encoded fluorescence marker due to its autocatalytic formation of the chromophore. In recent years, numerous GFP-like proteins with emission colors ranging from cyan to red were discovered in marine organisms. Their diverse molecular properties enabled novel approaches in live cell imaging but also impose certain limitations on their applicability as markers. In this review, we give an overview of key structural and functional properties of fluorescent proteins that should be considered when selecting a marker protein for a particular application and also discuss challenges that lie ahead in the further optimization of the glowing probes.

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Section: Fp-induced Cytotoxicitymentioning

confidence: 99%

Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

Wiedenmann¹,

Oswald²,

Nienhaus³

2009

IUBMB Life

218

162

View full text Add to dashboard Cite

show abstract

“…Cell culture and visualization was performed as described previously (Mustafa et al, 2006). Cell culture and visualization was performed as described previously (Mustafa et al, 2006).…”

Section: Cell Assaysmentioning

confidence: 99%

Ribosome display and selection of human anti-CD22 scFvs derived from an acute lymphocytic leukemia patient

Rothe

Nathanielsz

Oberhäuser

et al. 2008

BCHM

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Novel in vitro methods for the display of antibody libraries against disease-related antigens have led to the development of powerful protein-based biotherapeutics. Eukaryotic ternary ribosome complexes can be used to display human single chain antibodies (scFvs) to isolate specific binding reagents to these antigens. Here, we present the isolation of human scFv against the immunotherapeutic target antigen CD22 from a patient-derived human scFv library using ribosome display technology. The ribosome complexes were enriched against the extra-cellular domain of human CD22 conjugated to magnetic beads. Isolated constructs were further affinity-matured and specific binding activity was demonstrated by surface plasmon resonance and validated using in vitro cell assays. The isolated human anti-CD22 scFvs can provide a basis for the development of new immunotherapeutic strategies in CD22-expressing malignant diseases.

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Biotransport and intracellular ice formation phenomena in freezing human embryonic kidney cells (HEK293T)

Zhao

Zhou

et al. 2014

Cryobiology

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Identification of a functional nuclear export signal in the green fluorescent protein asFP499

Cited by 3 publications

References 16 publications

Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

Ribosome display and selection of human anti-CD22 scFvs derived from an acute lymphocytic leukemia patient

Biotransport and intracellular ice formation phenomena in freezing human embryonic kidney cells (HEK293T)

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