Identification of a Fatty Acyl-CoA Synthetase Gene,lcf2+, Which Affects Viability after Entry into the Stationary Phase inSchizosaccharomyces pombe

Fujita, Yasuyuki; Mita, Satoka; Ohtsuka, Hokuto; Aiba, Hirofumi

doi:10.1271/bbb.70442

Cited by 19 publications

(20 citation statements)

References 17 publications

(14 reference statements)

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“…The mcl1 + gene encodes a polymerase alpha accessory protein, so its effect on the spore wall is likely indirect (Williams and McIntosh 2005). lcf2 + encodes a predicted fatty-acyl CoA ligase, which could influence the composition of cellular membranes (Fujita et al 2007). The stress sensitivity and iodine staining defects in these cells may reflect an influence of lcf2 + on the activity or delivery of the integral membrane Mok14 alpha-glucan synthase responsible for synthesis of the iodine-reactive polymer (Garcia et al 2006).…”

Section: Resultsmentioning

confidence: 99%

A Genome-Wide Screen for Sporulation-Defective Mutants inSchizosaccharomyces pombe

Ucisik-Akkaya

Leatherwood

Neiman

2014

G3 Genes|Genomes|Genetics

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Yeast sporulation is a highly regulated developmental program by which diploid cells generate haploid gametes, termed spores. To better define the genetic pathways regulating sporulation, a systematic screen of the set of ~3300 nonessential Schizosaccharomyces pombe gene deletion mutants was performed to identify genes required for spore formation. A high-throughput genetic method was used to introduce each mutant into an h90 background, and iodine staining was used to identify sporulation-defective mutants. The screen identified 34 genes whose deletion reduces sporulation, including 15 that are defective in forespore membrane morphogenesis. In S. pombe, the total number of sporulation-defective mutants is a significantly smaller fraction of coding genes than in S. cerevisiae, which reflects the different evolutionary histories and biology of the two yeasts.

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Section: Resultsmentioning

confidence: 99%

A Genome-Wide Screen for Sporulation-Defective Mutants inSchizosaccharomyces pombe

Ucisik-Akkaya

Leatherwood

Neiman

2014

G3 Genes|Genomes|Genetics

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“…We reported previously that a mutant of lcf1 ϩ , which encodes a long-chain fatty acyl-CoA synthetase, showed rapid loss of viability after entry into stationary phase and suggested that fatty acid utilization and/or metabolism is important to determine viability in stationary phase in fission yeast (8,9). Recently, we identified a novel gene, ecl1 ϩ , which extends the chronological life span of S. pombe when overexpressed (10).…”

mentioning

confidence: 99%

Pma1, a P-type Proton ATPase, Is a Determinant of Chronological Life Span in Fission Yeast

Ito

Oshiro

Fujita

et al. 2010

Journal of Biological Chemistry

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Chronological life span is defined by how long a cell can survive in a non-dividing state. In yeast, it is measured by viability after entry into stationary phase. To date, some factors affecting chronological life span have been identified; however, the molecular details of how these factors regulate chronological life span have not yet been elucidated clearly. Because life span is a complicated phenomenon and is supposedly regulated by many factors, it is necessary to identify new factors affecting chronological life span to understand life span regulation. To this end, we have screened for long-lived mutants and identified Pma1, an essential P-type proton ATPase, as one of the determinants of chronological life span. We show that partial loss of Pma1 activity not only by mutations but also by treatment with the Pma1 inhibitory chemical vanadate resulted in the longlived phenotype in Schizosaccharomyces pombe. These findings suggest a novel way to manipulate chronological life span by modulating Pma1 as a molecular target.In the natural environment, most microorganisms exhibit only brief periods of rapid growth. Nutrient starvation is the most common natural situation, so the ability to adapt to nutrient limitation is crucial for microorganisms. Cells respond to starvation by ceasing growth and entering stationary phase and differentiate in ways that allow them to maintain viability for extended periods in the absence of nutrients (1). In yeast, the period in which the cells keep their viability after entry into stationary phase is recognized as the chronological life span (2).In Saccharomyces cerevisiae, two of the major pathways that control chronological life span have been identified: the Ras-PKA-Msn2/4 pathway and the Sch9 pathway (3, 4). The downregulation of either pathway promotes life span extension. Importantly, similar pathways (insulin/insulin-like growth factor I-like) regulate longevity in higher eukaryotes, suggesting a common evolutionary origin for the life span regulatory mechanisms (5, 6).In Schizosaccharomyces pombe, Pka1 and Sck2 are regulators of chronological life span; each mutant shows a long-lived phenotype, and the pka1 sck2 double mutant displays an additive effect on chronological life span extension, suggesting that these two factors regulate related but independent pathways (7). We reported previously that a mutant of lcf1 ϩ , which encodes a long-chain fatty acyl-CoA synthetase, showed rapid loss of viability after entry into stationary phase and suggested that fatty acid utilization and/or metabolism is important to determine viability in stationary phase in fission yeast (8,9). Recently, we identified a novel gene, ecl1 ϩ , which extends the chronological life span of S. pombe when overexpressed (10). In addition, we also identified two paralogs and one ortholog of ecl1 ϩ in S. pombe and S. cerevisiae, respectively (11, 12). On the basis of these observations, we proposed that Ecl1 family proteins are novel regulators for chronological life span in yeast. Ecl1 family proteins ...

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“…In S. pombe, calorie restriction is conducted by culturing cell in 0.5% glucose media instead of 2% glucose. Under this condition, chronological lifespan of fission yeast extended as like in other organisms (Fujita et al 2007). When ecl1 ?…”

Section: Resultsmentioning

confidence: 70%

“…Previously we identified genes involved in chronological lifespan in Schizosaccharomyces pombe (Oshiro et al 2003;Fujita et al 2007;Ohtsuka et al 2008Ohtsuka et al , 2009). Both lcf1 ?…”

Section: Introductionmentioning

confidence: 99%

hsf1+ extends chronological lifespan through Ecl1 family genes in fission yeast

et al. 2010

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The heat shock factor (HSF), a protein evolutionarily conserved from yeasts to human, regulates the expression of a set of proteins called heat shock proteins (HSPs), many of which function as molecular chaperones. In Saccharomyces cerevisiae, the HSF binds to the 5' upstream region of YGR146C and activates its transcription. YGR146C encodes a functional homolog of ecl1 (+), ecl2 (+), and ecl3 (+) of Schizosaccharomyces pombe. At present, these Ecl1 family genes, which are extenders of chronological lifespan, have been identified only in fungi groups. Based on ChIP analysis, we identified that Hsf1 binds to the upstream DNA region of ecl2 (+) after heat shock in S. pombe. In Caenorhabditis elegans, heat shock factor HSF-1 is known to regulate aging and required for the elongation of longevity by dietary restriction. We found that heat shock factor Hsf1 extends chronological lifespan of S. pombe when overexpressed. Moreover, we show that the extension of chronological lifespan by the overproduction of Hsf1 mainly depends on ecl2 (+) among Ecl1 family genes. From these results, we suggest that HSF is a conserved regulator of lifespan, at least in yeast and nematode, and Ecl1 family genes such as YGR146C and ecl2 (+) are the direct targets of Hsf1 and mediate lifespan extension by Hsf1.

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Identification of a Fatty Acyl-CoA Synthetase Gene,lcf2⁺, Which Affects Viability after Entry into the Stationary Phase inSchizosaccharomyces pombe

Cited by 19 publications

References 17 publications

A Genome-Wide Screen for Sporulation-Defective Mutants inSchizosaccharomyces pombe

A Genome-Wide Screen for Sporulation-Defective Mutants inSchizosaccharomyces pombe

Pma1, a P-type Proton ATPase, Is a Determinant of Chronological Life Span in Fission Yeast

hsf1+ extends chronological lifespan through Ecl1 family genes in fission yeast

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