Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

Talluri, Subha; Bhatt, Anand; Smith, Dean P.

doi:10.1073/pnas.92.25.11475

Cited by 74 publications

(65 citation statements)

References 45 publications

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“…We suspect that this clone is expressed by most neurons in the lobster, and probably by other cell types as well. The most highly homologous member of the Ga~1subfamily, Drosophila Ga~3,is also expressed in a wide variety of neuronal and nonneuronal tissues, including a subset of ORNs (Talluri et al, 1995). We have tested an antiserum specific for residues 306-319 of f)rosophila Ga5.3, but it detected no bands in western blots using lobster tissues.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Cloning of a Lobster Gα_q Protein Expressed in Neurons of Olfactory Organ and Brain

McClintock

Quintero

et al. 1997

Journal of Neurochemistry

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Abstract:We have isolated from an American lobster (Homarus americanus) olfactory organ cDNA library a clone, hGaq, with >80% identity to mammalian and arthropod Gaq sequences. In brain and olfactory organ, hGcsq mRNA was expressed predominantly in neurons, including virtually all the neuronal cell body clusters of the brain. Gaq protein was also expressed broadly, appearing on western blots as a single band of 46 kDa in brain, eyestalk, pereiopod, dactyl, tail muscle, olfactory organ, and aesthetasc hairs. These results suggest that hGaq plays a role in a wide variety of signal transduction events. Its presence in the olfactory aesthetasc hairs, which are almost pure preparations of the outer dendrites of the olfactory receptor neurons, the expression of a single hGcsq mRNA species (6 kb) in the olfactory organ, and the localization of hGaq mRNA predominantly in the olfactory receptor neurons of the olfactory organ strongly suggest that one function of hGaq is to mediate olfactory transduction. Key Words: Olfaction-G protein-Signal transduction -Crustaceans-Lobster. J. Neurochem. 68, 2248-2254 (1997).Olfactory transduction can be mediated by either the cyclic adenosine 3 '-monophosphate (cAMP) or the i nositol 1 ‚4,5-trisphosphate (InsP 3) pathways, with odorants activating both in most species (Ache, 1994;Dionne and Dubin, 1994). The roles such dual transduction pathways might play depend on whether they interact within individual olfactory receptor neurons (ORNs) or whether they are separated by odor sensitivity, odor concentration dependence, or exclusive expression patterns. In mammalian ciliary membrane preparations, there is a complete separation of the odor sensitivity of the cAMP and InsP1 pathways (Boekhoff et al., 1990; Breer and Boekhoff, 1991), although ORNs in culture may show activation of both pathways by an odorant (Ronnett et al., 1991). However, the total absence of odor responses in mice lacking functional olfactory cyclic nucleotide-gated channels suggests that the (lnsP3) pathway is not a primary transduction pathway (Brunet et al., 1996). Although these conflicting results raise doubts about the role of InsP3 in olfactory transduction in mammals, results from other species are more definitive. In catfish, both pathways are activated by odorants (Huque and Bruch, 1986;Bruch and Teeter, 1990) and conductances activated by each appear to occur in virtually all catfish ORNs (Miyamoto et al., 1992). This suggests that both pathways can coexist in a single ORN. In lobster ORNs this coexistence was established in single-cell recordings of the summation of depolarizing receptor potentials caused by odor-evoked InsPproduction and hyperpolarizing receptor potentials caused by odorevoked cAMP production (McClintock and Ache, 1989b;Michel et al., 1991;Fadool and Ache, 1992;Boekhoff et al., 1994;Hatt and Ache, 1994;Michel and Ache, 1994). The discovery that virtually all odorants can cause depolarizations in one lobster ORN but hyperpolarizations in another supports the hypothesis that this interactio...

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Section: Discussionmentioning

confidence: 99%

“…(, (Neer, 1995). In arthropods, one Drosophila Ga5 gene has been identified and it gives rise to three alternative splice products (Lee et al, 1990;Scott et al, 1995;Talluri et al, 1995). The Drosophila Gaq3 splice version of this gene is expressed in a wide variety of neuronal tissues including a subset of the ORNs.…”

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confidence: 99%

Molecular Cloning of a Lobster Gα_q Protein Expressed in Neurons of Olfactory Organ and Brain

McClintock

Quintero

et al. 1997

Journal of Neurochemistry

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“…Additional studies will be required to determine whether other Gα subunits are involved in gustatory signaling. For example, the Gα subunit DGqα-3 is also expressed in a subset of GRNs, suggesting a role in taste transduction [92].…”

Section: Signal Transductionmentioning

confidence: 99%

Taste and pheromone perception in the fruit fly Drosophila melanogaster

Ebbs

Amrein

2007

Pflugers Arch - Eur J Physiol

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Taste is an essential sense for detection of nutrient-rich food and avoidance of toxic substances. The Drosophila melanogaster gustatory system provides an excellent model to study taste perception and taste-elicited behaviors. "The fly" is unique in the animal kingdom with regard to available experimental tools, which include a wide repertoire of molecular-genetic analyses (i.e., efficient production of transgenics and gene knockouts), elegant behavioral assays, and the possibility to conduct electrophysiological investigations. In addition, fruit flies, like humans, recognize sugars as a food source, but avoid bitter tasting substances that are often toxic to insects and mammals alike. This paper will present recent research progress in the field of taste and contact pheromone perception in the fruit fly. First, we shall describe the anatomical properties of the Drosophila gustatory system and survey the family of taste receptors to provide an appropriate background. We shall then review taste and pheromone perception mainly from a molecular genetic perspective that includes behavioral, electrophysiological and imaging analyses of wild type flies and flies with genetically manipulated taste cells. Finally, we shall provide an outlook of taste research in this elegant model system for the next few years.Keywords Drosophila . Gustatory receptor neurons . GTP-binding protein-coupled receptors Like most animals, Drosophila monitor their chemical world with two distinct senses: the olfactory system, which is used for the detection of volatile chemicals, and the gustatory (taste) system, which enables the fly to detect soluble compounds. The olfactory sensory system (or the fly nose) is comprised of two pairwise head appendages, the third segments of the antennae, and the maxillary palps (Fig. 1). These appendages are covered with hundreds of sensory hairs, each of which contains two to four olfactory sensory neurons (OSNs) [1]. In contrast, the gustatory system is widely distributed over the animal's body. The main taste organ, the proboscis or labial palps (i.e., the fly tongue), is located on the distal end of the labellum (also a head appendage), but flies, like many other insects, have taste sensilla on legs and wings and, in females, on the genitalia [1,2].The roles of the olfactory and taste systems are clearly separated with regard to the physical state of chemicals they detect (volatile vs solubilized). However, the two systems often cooperate to fulfill specific functions in related processes and behaviors, the most obvious of them being the location and identification of food. For example, chemical cues such as the specific odor of a flower and the sugar compounds present in its nectar are by nature associated with one another. Thus, the odor cue provides a primary sensory input for an insect such as a honeybee to locate a food source (nectar), whose particular chemical composition is subsequently verified by the taste system. Similarly, the typical odor of yeast, which produces high amounts of t...

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“…These studies together favor the possibility of differential activation of dual transduction systems involving both G s ␣ (via cAMP) and G q ␣ (via InsP 3 and other phospholipids) during olfactory sensing (Ache and Young, 2005). Moreover, expression of G q ␣ RNA was demonstrated in a subset of olfactory sensory neurons (OSNs) (Talluri et al, 1995) and its knockdown by targeted RNA interference (RNAi) resulted in behavioral deficits (Kalidas and Smith, 2002).…”

Section: Introductionmentioning

confidence: 99%

Reduced Odor Responses from Antennal Neurons of G_qα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction

Kain¹,

Chakraborty²,

Sundaram³

et al. 2008

J. Neurosci.

110

117

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Mechanisms by which G-protein-coupled odorant receptors transduce information in insects still need elucidation. We show that mutations in the Drosophila gene for G q ␣ (dgq) significantly reduce both the amplitude of the field potentials recorded from the whole antenna in responses to odorants as well as the frequency of evoked responses of individual sensory neurons. This requirement for G q ␣ is for adult function and not during antennal development. Conversely, brief expression of a dominant-active form of G q ␣ in adults leads to enhanced odor responses. To understand signaling downstream of G q ␣ in olfactory sensory neurons, genetic interactions of dgq were tested with mutants in genes known to affect phospholipid signaling. dgq mutant phenotypes were further enhanced by mutants in a PLC␤ (phospholipase C␤) gene, plc21C. Interestingly although, the olfactory phenotype of mutant alleles of diacylglycerol kinase (rdgA) was rescued by dgq mutant alleles. Our results suggest that G q ␣-mediated olfactory transduction in Drosophila requires a phospholipid second messenger the levels of which are regulated by a cycle of phosphatidylinositol 1,4-bisphosphate breakdown and regeneration.

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Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

Abstract: We have identified another Drosophila GTP-

Cited by 74 publications

References 45 publications

Molecular Cloning of a Lobster Gα_q Protein Expressed in Neurons of Olfactory Organ and Brain

Molecular Cloning of a Lobster Gα_q Protein Expressed in Neurons of Olfactory Organ and Brain

Taste and pheromone perception in the fruit fly Drosophila melanogaster

Reduced Odor Responses from Antennal Neurons of G_qα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction

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Identification of a Drosophila G protein alpha subunit (dGq alpha-3) expressed in chemosensory cells and central neurons.

Abstract: We have identified another Drosophila GTP-

Cited by 74 publications

References 45 publications

Molecular Cloning of a Lobster Gαq Protein Expressed in Neurons of Olfactory Organ and Brain

Molecular Cloning of a Lobster Gαq Protein Expressed in Neurons of Olfactory Organ and Brain

Taste and pheromone perception in the fruit fly Drosophila melanogaster

Reduced Odor Responses from Antennal Neurons of Gqα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction

Contact Info

Product

Resources

About

Molecular Cloning of a Lobster Gα_q Protein Expressed in Neurons of Olfactory Organ and Brain

Molecular Cloning of a Lobster Gα_q Protein Expressed in Neurons of Olfactory Organ and Brain

Reduced Odor Responses from Antennal Neurons of G_qα, Phospholipase Cβ, andrdgAMutants inDrosophilaSupport a Role for a Phospholipid Intermediate in Insect Olfactory Transduction