Identification of a Drosophila brain-gut peptide related to the neuropeptide Y family

Brown, Mark R.; Crim, Joe W.; Arata, Ryan C; Cai, Houjian; Cao, Chun; Shen, Ping

doi:10.1016/s0196-9781(99)00097-2

Cited by 221 publications

(176 citation statements)

References 21 publications

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“…Dipteran NPF expression is observed in larval and adult brain tissue and endocrine cells in the midgut [93,94]. We could not detect mature NPF in the brain or midgut.…”

Section: Neuropeptide Fcontrasting

confidence: 63%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Neuropeptides and peptide hormones are essential signaling molecules that regulate nearly all physiological processes. The recent release of the tsetse fly genome allowed the construction of a detailed in silico neuropeptide database (International Glossina Genome Consortium, Science 344, 380-386 (2014)), as well as an in-depth mass spectrometric analysis of the most important neuropeptidergic tissues of this medically and economically important insect species. Mass spectrometric confirmation of predicted peptides is a vital step in the functional characterization of neuropeptides, as in vivo peptides can be modified, cleaved, or even mispredicted. Using a nanoscale reversed phase liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer, we detected 51 putative bioactive neuropeptides encoded by 19 precursors: adipokinetic hormone (AKH) I and II, allatostatin A and B, capability/ pyrokinin (capa/PK), corazonin, calcitonin-like diuretic hormone (CT/DH), FMRFamide, hugin, leucokinin, myosuppressin, natalisin, neuropeptide-like precursor (NPLP) 1, orcokinin, pigment dispersing factor (PDF), RYamide, SIFamide, short neuropeptide F (sNPF) and tachykinin. In addition, propeptides, truncated and spacer peptides derived from seven additional precursors were found, and include the precursors of allatostatin C, crustacean cardioactive peptide, corticotropin releasing factor-like diuretic hormone (CRF/DH), ecdysis triggering hormone (ETH), ion transport peptide (ITP), neuropeptide F, and proctolin, respectively. The majority of the identified neuropeptides are present in the central nervous system, with only a limited number of peptides in the corpora cardiaca-corpora allata and midgut. Owing to the large number of identified peptides, this study can be used as a reference for comparative studies in other insects.

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“…Dipteran NPF expression is observed in larval and adult brain tissue and endocrine cells in the midgut [93,94]. We could not detect mature NPF in the brain or midgut.…”

Section: Neuropeptide Fcontrasting

confidence: 63%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…Analysis of the three vertebrate PRXa peptides, NMU, AVP, and PP showed that the PRXa motifs are strictly conserved in NMU and AVP, whereas that of PP is likely a consequence of converging evolution from NPY͞PYY͞PP family, which includes Drosophila neuropeptide F [C-terminal motif (PH)R(YF)amide] (29,30). In this fashion, our search for PRXa-activated GPCRs in Drosophila was narrowed to those related to the AVP and NMURs (15)(16)(17).…”

Section: Bioinformatics: Identification Of Drosophila ؊Prxamide Peptimentioning

confidence: 99%

Identification of G protein-coupled receptors for Drosophila PRXamide peptides, CCAP, corazonin, and AKH supports a theory of ligand-receptor coevolution

Park

Kim

Adams

2002

Proc. Natl. Acad. Sci. U.S.A.

353

273

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G-protein coupled receptors (GPCRs) are ancient, ubiquitous sensors vital to environmental and physiological signaling throughout organismal life. With the publication of the Drosophila genome, numerous “orphan” GPCRs have become available for functional analysis. Here we characterize two groups of GPCRs predicted as receptors for peptides with a C-terminal amino acid sequence motif consisting of −PRXamide (PRXa). Assuming ligand-receptor coevolution, two alternative hypotheses were constructed and tested. The insect PRXa peptides are evolutionarily related to the vertebrate peptide neuromedin U (NMU), or are related to arginine vasopressin (AVP), both of which have PRXa motifs. Seven Drosophila GPCRs related to receptors for NMU and AVP were cloned and expressed in Xenopus oocytes for functional analysis. Four Drosophila GPCRs in the NMU group (CG11475, CG8795, CG9918, CG8784) are activated by insect PRXa pyrokinins, (−FXPRXamide), Cap2b-like peptides (−FPRXamide), or ecdysis triggering hormones (−PRXamide). Three Drosophila GPCRs in the vasopressin receptor group respond to crustacean cardioactive peptide (CCAP), corazonin, or adipokinetic hormone (AKH), none of which are PRXa peptides. These findings support a theory of coevolution for NMU and Drosophila PRXa peptides and their respective receptors

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“…Acceptance of food based on food quality as well as feeding status of the animal also seems to be regulated by the centrally and peripherally expressed neuropeptide F (npf) and its receptor (npfr; Brown et al 1999, Shen & Cai 2001, Wu et al 2003, 2005a. Recent work on the Drosophila npf/npfr system has demonstrated its involvement in suppressing aversion to noxious food in starved larvae (Wu et al 2005a) as well as in regulating hunger-driven motivational feeding on less accessible solid food (Wu et al 2005b).…”

Section: Neuromedins and Huginmentioning

confidence: 99%

“…It remains speculative whether or how, in these mutants, the overactive hugin system overrides npf signaling to cause stop of feeding. One difficulty in integrating the role of the npf/npfr system into central regulation of the feeding behavior in Drosophila is its peripheral expression in neuroendocrine cells in the gut (Brown et al 1999), an issue that has not yet been taken into account at all.…”

Section: Neuromedins and Huginmentioning

confidence: 99%

Amino acids, taste circuits, and feeding behavior in Drosophila: towards understanding the psychology of feeding in flies and man

Melcher¹,

Bader²,

Pankratz³

2007

Journal of Endocrinology

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Feeding can be regulated by a variety of external sensory stimuli such as olfaction and gustation, as well as by systemic internal signals of feeding status and metabolic needs. Faced with a major health epidemic in eating-related conditions, such as obesity and diabetes, there is an ever increasing need to dissect and understand the complex regulatory network underlying the multiple aspects of feeding behavior. In this minireview, we highlight the use of Drosophila in studying the neural circuits that control the feeding behavior in response to external and internal signals. In particular, we outline the work on the neuroanatomical and functional characterization of the newly identified hugin neuronal circuit. We focus on the pivotal role of the central nervous system in integrating external and internal feeding-relevant information, thus enabling the organism to make one of the most basic decisions -to eat or not to eat.

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Identification of a Drosophila brain-gut peptide related to the neuropeptide Y family

Cited by 221 publications

References 21 publications

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Identification of G protein-coupled receptors for Drosophila PRXamide peptides, CCAP, corazonin, and AKH supports a theory of ligand-receptor coevolution

Amino acids, taste circuits, and feeding behavior in Drosophila: towards understanding the psychology of feeding in flies and man

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