Identification of a distinct subpopulation of fibroblasts from murine dermis: CD73⁻CD105⁺ as potential marker of dermal fibroblasts subset with multipotency

Abstract

Skin dermis includes various types of multipotent stromal cells (MSCs) and a subpopulation of dermal fibroblasts that exhibit the ability to differentiate. However, characterization of this dermal fibroblast subtype remains less understood. In this study, we isolated dermal cells from the skin of newborn C57/B6 mice and investigated their characteristics. Isolated murine dermal cells exhibited a fibroblast phenotype as judged by accepted criteria including a lack of MSC-related antigens and the differentiation potential of MSCs, and the positive expression of fibroblast markers. A comparative analysis demonstrated that CD73(-) CD105(+) but not CD73(-) CD105(-) dermal fibroblasts exhibited some of the functional properties of MSCs. Furthermore, the multipotent phenotype of CD73(-) CD105(+) cells was diminished by treatment of CD105 siRNA and shRNA, indicating that CD105 expression was critical for the retention of differentiation potential of those cells. Overall, these results suggest that CD73(-) CD105(+) cells are a distinct subset of dermal fibroblasts with multipotency and that their surface antigens could help to classify this subpopulation. These cells may contribute to the regeneration of damaged tissue.

Insight into skin cell-based osteogenesis: a review

“…For example, type IV collagen-coated dishes have been used to attract CD29(+) human dermal stem cells via adherence, which exhibited higher osteogenic, adipogenic, and chondrogenic capacity compared with unsorted cells 33 . CD271(+) and CD146(+) cells isolated from human skin and CD73(−)CD105(+) cells isolated from mouse skin by immunosorting also showed elevated multi-differentiation potential 50– 52 . Interestingly, subpopulations sorted by other markers from human skin, such as CD73, stage-specific embryonic antigen-4 (SSEA-4), and BmprIB, show relatively restricted differentiation potential.…”

“…(F) Interfollicle epidermis-derived stem cells 13, 14 . (G) Stem cells from dermal niches that are not fully characterized 27– 34, 50, 52, 53 . (H–K) Strategies for using skin cells.…”

Utilizing Human Dermal Fibroblast Heterogeneity in Autologous Dermal Fibroblast Therapy: An Overcomplicated Strategy or a Promising Approach?

“…Third, the most common species in which DF heterogeneity research is performed is humans. Recently, there have also been some reports of DF heterogeneity in pigs (Zuo et al, ; Zuo and Lu, ) and mice (Collins et al, ; Lee et al, ).…”

“…Thereafter, the remaining whole dermis is minced finely and subjected to primary cell culture. Finally, the DF subpopulation is sorted using a fluorescence‐activated cell sorter based on cell surface molecules (Collins et al, ; Lee et al, ; Philippeos et al, ).…”

“…However, we could find no other studies that used these markers in the literature, and those that are available do not appear to have been validated. Third, the cellular markers of DF used in mouse skin are commonly related to lineage tracing and therefore generally cannot be reproduced in humans (Driskell et al, ; Rinkevich et al, ; Lee et al, ).…”