Identification of a congenital defect of factor VII in a colony of beagle dogs: the clinical use of the plasma

Abstract

A severe, pure deficiency of factor VII has been defined in haematological and biochemical terms in the Alderley Park colony of beagle dogs. By modification of the conventional factor VII assay to improve its specificity an apparent low activity of between 1 and 2·5% of the normal beagle has been found.When this plasma is used as a substrate for factor VII assays it compares well with human factor VII-deficient plasma in the measurement of both raised and depressed factor VII activity. It also appears to be a valuable reagent in the quality control of tissue thromboplastin extracts.

Factor VII as a marker of hepatocellular synthetic function in liver disease.

“…It must not be assumed, however, that this will be the case if the prothrombin time is performed with a different type of thromboplastin reagent, as it has been established that the BCT possesses greater sensitivity to factor VII than commercial thromboplastin reagents used in the Quick test (Zucker et al, 1970;Poller et al, 1971). It is thus reassuring that this study indicates that the conventional coagulation test available to all British hospitals provides adequate assessment of hepatic clotting factor synthesis in patients with chronic liver disease.…”

“…Factor VII assays were performed using a single batch of lyophilized plasma from congenital factor VII deficient beagle dogs (Poller et al, 1971). 971 G. Green, L. Poller, Jean M. Thomson, and I. W. Dymock For an analysis of the results, patients were grouped diagnostically and the relationships between factor VII levels, serum albumin, one-stage prothrombin time, and bromosulphthalein retention were correlated.…”

“…plasma are severely restricted it was important to the liver, has a evaluate if any important contribution was made by other clotting specific factor VII assays in the management of urs (Loeliger et factor VII is usually employed in this test (Poller et al, 1971;Zucker et al, 1970). We have therefore compared the relative value of the specific factor VII assay with the broad-spectrum test, the prothrombin time using BCT, in the various groups with chronic liver disease.…”

“…However, the availability of a naturally occurring factor VII deficient plasma in beagle dogs (Poller et al, 1971) has provided a standardized specific clotting factor VII assay. Its importance in accurately reflecting the state of hepatic function in Received for publication 8 April 1976 clinical situations, particularly acute hepatic failure, has recently been reported (Poller et al, 1971;Dymock et al, 1975).…”

Effects of Tranexamic Acid on the Coagulation and Fibrinolytic Systems in Pregnancy Complicated by Placental Bleeding

“…The following tests were carried out: platelet count on a sequestrene sample using a coulter counter, fibrinolytic activity on fibrin plates (Kluft et al 1976), factor VIII (Biggs et al 1955), factor VIII related antigen (Laurell1966), factor VII (Poller et al 1971), fibrinogen (Clauss 1957), prothrombin time (Thomson 1980), kaolin cephalin clotting time (Thomson 1980), factor X, anti Xa, antithrombin III, antiplasmin and plasminogen were all determined biologically using Kabi chromogenic substrates.…”