Identification of a codominant scar marker linked to the seedlessness character in grapevine

Lahogue, F.; This, P.; Bouquet, A.

doi:10.1007/s001220050976

Cited by 110 publications

(97 citation statements)

References 14 publications

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“…BSA was first used to identify markers for disease resistance genes in lettuce species (Michelmore et al 1991). Since then the technique has been used in many studies for identification of markers in different crop species such as apple (Yang et al 1997), grapevine (Lahogue et al 1998), and maize (Quarrie et al 1999). A similar approach to ours identified 4 markers closely linked to the Rsv1 locus of soybean mosaic virus (Hayes and Saghai Maroof 2000).…”

Section: Discussionmentioning

confidence: 59%

Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.)

Çakır¹,

Gupta²,

Platz³

et al. 2003

Aust. J. Agric. Res.

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Abstract. Identification and deployment of disease resistance genes are key objectives of Australian barley breeding programs. Two doubled haploid (DH) populations derived from Tallon × Kaputar (TK) and VB9524 × ND11231 (VN) crosses were used to identify markers for net type net blotch (NTNB) (Pyrenophora teres f. teres). The maps included 263 and 250 markers for TK and VN populations, respectively. The TK population was screened with 5 pathotypes and the VN population with 1 pathotype of NTNB as seedlings in the glasshouse. In addition, the TK population was subjected to natural infection in the field at Hermitage Research Station, Qld. Analyses of the markers were performed using the software packages MapManager and Qgene. One region on chromosome 6H was strongly associated with resistance to NTNB in both populations (R 2 = 83% for TK and 66% for VN). In the TK population, 2 more quantitative trait loci (QTLs) were identified on chromosomes 2H and 3H, with R 2 values of 30% and 31%, respectively. These associations were consistent over all pathotypes studied during the seedling stage. The same QTL on chromosome 6H was also found to be highly significantly associated (R 2 = 65%) with the adult plant (field) response in the TK population. There are several very closely linked markers showing strong associations in these regions. Association of the 4 markers on chromosome 6H QTL with resistance to the NTNB has been validated in 2 other DH populations derived from barley crosses Pompadour × Stirling and WPG8412 × Stirling. These markers present an opportunity for marker assisted selection of lines resistant to NTNB in barley breeding programs.

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Section: Discussionmentioning

confidence: 59%

Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.)

Çakır¹,

Gupta²,

Platz³

et al. 2003

Aust. J. Agric. Res.

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“…The loss of polymorphism can be solved through the use of restriction enzymes, which promote the cut at specific sites in one allele of a given locus. The technique was successfully used by several authors, among them: Weng et al (1998), Lahogue et al (1998), Dax et al (1998) and Zhang & Stommel (2001). The choice of restriction enzyme based on the sequencing of the fragments SCAR.…”

Section: Scar (Sequence Characterized Amplified Regions)mentioning

confidence: 99%

Molecular Markers: Assisted Selection in Soybeans

Gavioli¹

2011

Soybean - Genetics and Novel Techniques for Yield Enhancement

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“…Several hypotheses have been proposed to explain the different segregation patterns observed in different crosses involving stenospermocarpic seedlessness (see Bouquet and Danglot 1996 for a review). One hypothesis postulated the presence of a dominant allele at a single locus, later named SDI for "seed development inhibitor" (Lahogue et al 1998), inhibiting the development of the seed by regulating several recessive genes (Bouquet and Danglot 1996). The existence of this locus has been confirmed by QTL mapping (Cabezas et al 2006;Costantini et al 2008;Doligez et al 2002;Mejía et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…To date, four molecular markers have been proposed for MAS for stenospermocarpic seedlessness. The first two were sequence-characterized amplified region (SCAR) markers derived from bulked segregant analyses (Michelmore et al 1991): SCC8 (Lahogue et al 1998) and SCF27 (Mejía and Hinrichsen 2003). Although these markers could help to predict seedlessness, the presence of null alleles and the weak linkage with the SDI locus compromises their use in many genetic backgrounds Korpas et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Marker assisted selection for seedlessness in table grape breeding

Karaagac

Vargas²,

Andrés³

et al. 2012

Tree Genetics & Genomes

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Seedlessness is one of the most appreciated traits in the table grape (Vitis vinifera L.). The development of new seedless varieties is expensive and time consuming, involving the generation and selection of thousands of hybrids each year. In seeded × seedless crosses, seedlessness commonly segregates 1:1, so molecular markers allowing for the early identification of plants that will produce seedless berries are very useful. This early selection can lead to savings in maintenance and evaluation costs, and allows additional space for larger effective progenies. The variety Sultanina has been the main source of stenospermocarpic seedlessness in table grape breeding. In a previous work, we showed a 198-bp allele at the VMC7F2 microsatellite locus as a potential marker for selection of seedless genotypes due to its close linkage to the major effect seedless QTL, SDI. In this study, we show that stenospermocarpic bred cultivars share a main haplotype around this locus not found in seeded cultivars, which facilitating the development and use of marker assisted selection (MAS) strategies for the selection of seedless plants. In this way, VMC7F2 on its own can be a very useful marker for selecting seedless individuals from segregating crosses. A MAS program based on the presence of the 198-bp allele at VMC7F2 allows the reduction of the progeny size to 54%, selecting most of the seedless individuals. In addition, our results show the existence of other possible sources of stenospermocarpic seedlessness that could provide alternative sources of genetic variation for breeding of this trait. Communicated by D. GrattapagliaElectronic supplementary material The online version of this article

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Identification of a codominant scar marker linked to the seedlessness character in grapevine

Cited by 110 publications

References 14 publications

Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.)

Mapping and validation of the genes for resistance to Pyrenophora teres f. teres in barley (Hordeum vulgare L.)

Molecular Markers: Assisted Selection in Soybeans

Marker assisted selection for seedlessness in table grape breeding

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