Identification of a bacteriocin-like compound from Lactobacillus plantarum with antimicrobial activity and effects on normal and cancerogenic human intestinal cells

Abstract

In this paper, we demonstrate that the antimicrobial activity of L. plantarum PBS067 strain against antagonist microorganisms was mediated by the production of a bacteriocin-like compound secreted at the stationary phase of the growth. The novel bacteriocin-like compound, designed plantaricin P1053, was identified by using sorption–desorption method, butanol extraction and SEC-HPLC. The molecular mass of plantaricin P1053 was shown to be 1053 Da by ESI-MS analysis. Plantaricin P1053 exhibited a broad-spectrum antimicrobial activity against Gram-positive bacteria as S. aureus and Gram-negative bacteria as E. coli . In addition to the antimicrobial activity, the isolated bacteriocin-like compound showed effects on normal and cancerogenic epithelial intestinal cell lines through an enhancing of viability of healthy cells and a proliferation reduction of cancer cells. Moreover, in this paper we demonstrate that the isolated bacteriocin-like compound acts on healthy cells through the epidermal growth factor receptor (EGFR) pathways. In conclusion, plantaricin P1053 isolated from L. plantarum PBS067 strain could represent one of the first multifunctional bacteriocin-like compound acting on human epithelial intestinal cells.

Bacteriostatic activity and partial characterization of the bacteriocin produced by L. plantarum sp. isolated from traditional sourdough

“…Bacteriocins produced by lactic acid bacteria (LAB) have been considered mainly due to their essential role in the fermentation process of food, the creation of a flavor, and food preservation (Costa et al.,; Gautam & Sharma, 2009). Furthermore, they are interesting due to their long history of use in food industries, and the generally regarded as safe (GRAS) and Qualified Presumption of Safety (QPS) status (Alvarez‐Sieiro, Montalbán‐López, Mu, & Kuipers, 2016; De Giani et al., 2019). The LAB family includes Lactobacillus , Lactococcus , Leuconostoc , Pediococcus , Streptococcus , Aerococcus , Alloiococcus , Carnobacterium , Dolosigranulum , Enterococcus , Oenococcus , Tetragenococcus , Vagococcus, and Weissella (Mokoena, 2017).…”

Growth inhibition of pathogenic microorganisms by Pseudomonas protegens EMM-1 and partial characterization of inhibitory substances

“…The antimicrobial activity of the crude extract was determined by the agar-well diffusion assay according to De Giani et al [ 36 ] with some modifications. Petri plates were poured with soft LB agar (8 g/L) inoculated with an indicator strain.…”

Characteristics of Heterologous Plantaricin from <i>Lactobacillus plantarum</i> and its Future in Food Preservation

“…Plantaricin P1053 isolated from L. plantarum PBS067 strain is one of the first multifunctional bacteriocin-like compounds to be found to act on human epithelial intestinal cells. The molecular mass of plantaricin P1053 is 1053 Da (De Giani et al, 2019). Plantaricin P1053 showed normal effect and cancerogenic epithelial intestinal cell lines through an enhancing of viability of healthy cells and a proliferation reduction of cancer cells.…”

“…The uses of plantaricin are widely studied and has developed rapidly, with diverse applications such as antibacterial packaging film (Yang et al, 2019), reduction of intestinal cancer cells (De Giani et al, 2019), bio-preservation of fresh fish and shellfish (Čanak et al, 2018), extending the shelf-life of food without altering the nutritional quality of products (Flórez and Mayo, 2018), anti-cancer drugs (Baindara et al, 2018), and active polyvinylidene chloride (PVDC) films as antimicrobial wrapping for fresh pork (Xie et al, 2018). Plantaricin products can lower cholesterol, act as antioxidants (Devi and Halami, 2019), and are active against Candida (Sharma and Srivastava, 2014).…”