Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein

Qian, Chao Nan; Qin, Di; Qiao, Tiankui; Zeng, Yi; Tang, Guixia; Lu, Chun Yi

doi:10.1007/s11262-005-0050-8

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…The HIV-1 Tat 101 gene was synthesized by multiple rounds of overlapping PCR based on the genome sequence of HIV-1 (GenBank accession number M14310.1) as described previously (2,50). The Flag M2 epitope was fused in frame to the carboxyl-terminal end of the Tat 101 open reading frame, a Kozak sequence (GCCACC) was added to the upstream of initiator codon ATG to enhance the expression of the target gene, and the synthesized sequences were engineered with the cut sites of HindIII restriction enzymes at the 5Ј and 3Ј ends, respectively, to facilitate cloning.…”

Section: Methodsmentioning

confidence: 99%

Intracellular Tat of Human Immunodeficiency Virus Type 1 Activates Lytic Cycle Replication of Kaposi's Sarcoma-Associated Herpesvirus: Role of JAK/STAT Signaling

Zeng

Zhang

Huang

et al. 2007

J Virol

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111

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Human immunodeficiency virus type 1 (HIV-1) infection significantly increases the risk of Kaposi's sarcoma (KS) occurrence in individuals infected with Kaposi's sarcoma-associated herpesvirus (KSHV). KSHV infection appears to be necessary but not sufficient for KS development without other cofactors. However, factors that facilitate KSHV to cause KS have not been well defined. Previously, we determined that human herpesvirus 6 was one of the cofactors that activated lytic cycle replication of KSHV. Here, we demonstrate that the Tat protein of HIV-1 is a potentially important factor in the pathogenesis of KS, as determined by production of lytic phase mRNA transcripts and viral proteins in BCBL-1 cells. Mechanistic studies showed ectopic expression of Tat induced the production of human interleukin-6 (huIL-6) and its receptor (huIL-6Ra) and activated STAT3 signaling. Neutralization of huIL-6 or huIL-6R or inhibition of STAT3 signaling enhanced the replication. In addition, IL-4/STAT6 signaling also partially contributed to Tat-induced KSHV replication. These findings suggest that Tat may participate in KS pathogenesis by inducing KSHV replication and increasing KSHV viral load. These data also suggest that JAK/STAT signaling may be of therapeutic value in AIDS-related KS patients.

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Section: Methodsmentioning

confidence: 99%

Intracellular Tat of Human Immunodeficiency Virus Type 1 Activates Lytic Cycle Replication of Kaposi's Sarcoma-Associated Herpesvirus: Role of JAK/STAT Signaling

Zeng

Zhang

Huang

et al. 2007

J Virol

Self Cite

111

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“…The immunization of 8 kittens with the recombinant M protein of the feline infectious peritonitis virus (FIPV) allowed 3 of them to survive the viral infection [ 56 ]. B-cell epitopes have also been found in the M protein of SARS-CoV as well as the avian infectious bronchitis virus [ 57 , 58 ]. It has also been experimentally demonstrated that some peptides of the SARS-CoV M protein are highly reactive with sera from the convalescent phase of SARS patients.…”

Section: Discussionmentioning

confidence: 99%

Predicted 3D model of the M protein of Porcine Epidemic Diarrhea Virus and analysis of its immunogenic potential

et al. 2022

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The membrane protein M of the Porcine Epidemic Diarrhea Virus (PEDV) is the most abundant component of the viral envelope. The M protein plays a central role in the morphogenesis and assembly of the virus through protein interactions of the M-M, M-Spike (S) and M-nucleocapsid (N) type. The M protein is known to induce protective antibodies in pigs and to participate in the antagonistic response of the cellular antiviral system coordinated by the type I and type III interferon pathways. The 3D structure of the PEDV M protein is still unknown. The present work exposes a predicted 3D model of the M protein generated using the Robetta protocol. The M protein model is organized into a transmembrane and a globular region. The obtained 3D model of the PEDV M protein was compared with 3D models of the SARS-CoV-2 M protein created using neural networks and with initial machine learning-based models created using trRosetta. The 3D model of the present study predicted four linear B-cell epitopes (RSVNASSGTG and KHGDYSAVSNPSALT peptides are noteworthy), six discontinuous B-cell epitopes, forty weak binding and fourteen strong binding T-cell epitopes in the CV777 M protein. A high degree of conservation of the epitopes predicted in the PEDV M protein was observed among different PEDV strains isolated in different countries. The data suggest that the M protein could be a potential candidate for the development of new treatments or strategies that activate protective cellular mechanisms against viral diseases.

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“…One of the critical roles of the M protein is inducing virus neutralization, which makes it an attractive target for developing vaccines, drugs, and diagnostic reagents ( Ujike and Taguchi, 2015 ; Jorrissen et al., 2021 ). Two recombinant M proteins, M fusion protein (aa 1-43) and cytoplasmic-domain (aa 138-222) of the M protein, have been applied to develop indirect ELISA to validate their potential diagnostic value for SARS ( Han et al., 2004 ; Carattoli et al., 2005 ; Qian et al., 2006 ). All of them were specifically reacted with sera from SARS-CoV positive patients, confirmed by western blot or immunocytochemical assay, even when the sera were diluted to 1:12,800.…”

Section: Immunoassay Methodsmentioning

confidence: 99%

Recent advances in immunoassay technologies for the detection of human coronavirus infections

Wang

Chen

Xiang

et al. 2023

Front. Cell. Infect. Microbiol.

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Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the seventh coronavirus (CoV) that has spread in humans and has become a global pandemic since late 2019. Efficient and accurate laboratory diagnostic methods are one of the crucial means to control the development of the current pandemic and to prevent potential future outbreaks. Although real-time reverse transcription-polymerase chain reaction (rRT-PCR) is the preferred laboratory method recommended by the World Health Organization (WHO) for diagnosing and screening SARS-CoV-2 infection, the versatile immunoassays still play an important role for pandemic control. They can be used not only as supplemental tools to identify cases missed by rRT-PCR, but also for first-line screening tests in areas with limited medical resources. Moreover, they are also indispensable tools for retrospective epidemiological surveys and the evaluation of the effectiveness of vaccination. In this review, we summarize the mainstream immunoassay methods for human coronaviruses (HCoVs) and address their benefits, limitations, and applications. Then, technical strategies based on bioinformatics and advanced biosensors were proposed to improve the performance of these methods. Finally, future suggestions and possibilities that can lead to higher sensitivity and specificity are provided for further research.

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Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein

Cited by 6 publications

References 34 publications

Intracellular Tat of Human Immunodeficiency Virus Type 1 Activates Lytic Cycle Replication of Kaposi's Sarcoma-Associated Herpesvirus: Role of JAK/STAT Signaling

Intracellular Tat of Human Immunodeficiency Virus Type 1 Activates Lytic Cycle Replication of Kaposi's Sarcoma-Associated Herpesvirus: Role of JAK/STAT Signaling

Predicted 3D model of the M protein of Porcine Epidemic Diarrhea Virus and analysis of its immunogenic potential

Recent advances in immunoassay technologies for the detection of human coronavirus infections

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