Identification of a 36‐kDa olive‐pollen allergen by <i>in vitro</i> and <i>in vivo</i> studies

Martı́nez, Alberto; Asturias, Juan A.; Palácios, Ricardo; Martı́nez, Jorge; Ml, Sanz; Sánchez, G.; Oehling, A

doi:10.1034/j.1398-9995.1999.00073.x

Cited by 20 publications

(10 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is an anemophilous tree with a pollen production of around 12,500 pollen grains by anther in the case of Fraxinus excelsior L. (Pohl 1937) and between 18,000 and 87,000 in the case of Fraxinus angustifolia Vahe (Tormo et al 1996). The allergological interest in Fraxinus pollen stems from its potential cross-reactivity with pollen from other Oleaceae, namely Olea pollen (Bousquet et al 1985, D'Amato 1998, Martínez et al 1999, Guerra et al 2000 and 100 pollen grains per cubic meter of air has been cited as threshold level to produce hay fever symptoms in Switzerland (Gehrig & Peeters 2000). The percentage of positive reactions to Fraxinus pollen in the population sensitive to a given pollen type is less than 8% in the south of Galicia (Arenas et al 1996).…”

mentioning

confidence: 99%

Heat and chill requirements ofFraxinusflowering in Galicia (NW Spain)

Jato

Rodríguez-Rajo

Dacosta

et al. 2004

Grana

View full text Add to dashboard Cite

mentioning

confidence: 99%

Heat and chill requirements ofFraxinusflowering in Galicia (NW Spain)

Jato

Rodríguez-Rajo

Dacosta

et al. 2004

Grana

View full text Add to dashboard Cite

“…It consists of a single 145-amino acid polypeptide chain and contains an N-linked glycosyl moiety. Besides this protein, an increasing number of IgE-binding proteins from olive pollen are being characterized [10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20]. In most of the cases reported in the literature, the working material used corresponds to commercially available mature pollen, composed of an unknown mixture of cultivars and origins.…”

Section: Introductionmentioning

confidence: 99%

Pollen from Different Olive Tree Cultivars Contains Varying Amounts of the Major Allergen Ole e 1

Castro

Alché

Cuevas

et al. 2003

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: Commercial olive pollen from uncertain cultivar origin is the common material used for clinical and biological studies. We aimed to assess the putative heterogeneity of olive cultivars with regard to the presence of the major pollen allergen Ole e 1 and to determine whether these differences have clinical relevance. Methods: The Ole e 1 content of several cultivars was determined by immunoblotting and ultrastructural immunocytochemistry and compared to that of a commercially available olive pollen extract designed for diagnosis. Reverse transcription-polymerase chain reaction analysis of Ole e 1 transcripts was also performed. Crude protein extracts were used to carry out skin prick tests (SPTs) on 30 allergic patients in order to evaluate the clinical importance of such differences. Results: Ole e 1 was present in all cultivars, although significant quantitative differences were detected. Ole e 1 transcripts positively correlated with the amount of the allergen. Significant variations in the average reactivity of allergic patients to SPTs were observed depending on the cultivar considered. Conclusions: The presence of the Ole e 1 allergen in all the cultivars suggests that this allergen may play an essential biological role. The expression of the allergen is controlled at the transcriptional level. The significant differences in the Ole e 1 content are likely responsible for the different average reactivity exhibited by patients to the cultivars studied, although the role of other allergens cannot be excluded. Our results suggest that the use of the commercial pollen mixtures currently available may lead to mistakes in allergy diagnosis and to limited success in immunotherapy. Therefore, further standardization is strongly recommended.

show abstract

“…Naše ponovljeno ispitivanje bolesnika s preosjetljivosti na pelud masline potvrdilo je i dalje izrazito nizak postotak preosjetljivosti od 8.8 %. Usporedba ekstrakta peluda masline u Kaliforniji i Španjolskoj pokazala je različitu zastupljenost proteina u antigenskoj strukturi peluda masline, što ima za posljedicu različitu ekspresiju preosjetljivosti na pelud masline u određenoj populaciji (10). Osim strukture samoga peludnog antigena, na nastanak preosjetljivosti i izražajnost kliničke slike mogu utjecati i drugi, ne manje važni činitelji.…”

Section: Raspravaunclassified

Hypersensitivity to Pollen of Olea Europea in Patients with Pollen Allergy in Zadar County, Croatia

Skitarelić

et al. 2010

Archives of Industrial Hygiene and Toxicology

View full text Add to dashboard Cite

Preosjetljivost na pelud masline važan je uzrok peludnih alergija u mediteranskim zemljama. Cilj ovoga rada bio je utvrditi učestalost preosjetljivosti na pelud masline među bolesnicima s peludnom alergijom u Zadru i Zadarskoj županiji. Dobivene rezultate usporedili smo s ranijim ispitivanjem preosjetljivosti na pelud masline u Dalmaciji. Ukupno je obrađen 671 ispitanik s preosjetljivosti na peludne alergene. Od toga broja 61 % ispitanika bilo je muškog spola, a 39 % je bilo ženskog spola. Od ukupno ispitanih 53.5 % činila su djeca u dobi od 4 do 14 godina, a preostalih 46.5 % bili su odrasli u dobi od 15 do 59 godina života. Svim ispitanicima uzeta je detaljna obiteljska i osobna anamneza, napravljen klinički pregled te učinjeno kožno prick-testiranje i enzimatsko-imunološki UniCAP-test za određivanje specifi čnih IgE-protutijela. Statistička obrada podataka učinjena je χ 2 -testom. Preosjetljivost na pelud masline dokazana je u 8.8 % bolesnika s peludnom alergijom. Najveći broj bolesnika s preosjetljivosti na pelud masline boluje od alergijskog rinitisa, 58 % bolesnika. Postoji statistički značajna razlika u broju bolesnika između dvije ispitivane sredine, gradske i seoske. Samo 3 % bolesnika stanovnici su otoka. Preosjetljivost na pelud masline u našem ispitivanju najniža je u odnosu na ispitivanja provedena u drugim mediteranskim zemljama. Najčešće se očituje kliničkom slikom alergijskog rinitisa, a statistički je značajno češća u gradskoj sredini. Usporedba preosjetljivosti na pelud masline tijekom dvaju ispitivanih razdoblja u našoj zemlji nije pokazala porast broja bolesnika s preosjetljivosti na pelud masline. Skitarelić N, et al. HYPERSENSITIVITY TO OLIVE POLLEN IN ZADAR Arh Hig Rada Toksikol 2010;61:211-217 Maslina (Olea europea) pripada porodici Oleacea. Razlikujemo dvije podvrste: Olea europea oleaster i Olea europea sativa (1). Prva se obično naziva divljom, a druga pitomom maslinom. Pradomovina masline prostire se između Sirije i Grčke, odakle je prenesena i raširena u zemlje Sredozemlja. Povijest maslinarstva u Hrvatskoj vrlo je stara i datira od I. i II. stoljeća naše ere (1). Osim masline u porodicu Oleacea spadaju i Fraxinus ornus, Ligustrum vulgare, Phyllyrea angustifolia. KLJUČNE RIJEČI: alergijski rinitis, atopija, Olea europea, polenozaRanija ispitivanja polenoza na području Zadra pokazala su da je od peludnih alergena kod naših bolesnika najčešća preosjetljivost na pelud trave Dactylis glomerata, dok je pelud stabala relativno rijedak uzrok preosjetljivosti (2). Ispitivanja preosjetljivosti na pelud masline u mediteranskim zemljama upućuju na maslinu kao važan uzrok peludne alergije (3-6). Rezultati ovih istraživanja potakli su nas na ispitivanje preosjetljivosti na pelud masline kod bolesnika u Dalmaciji (7), a u ovom ispitivanju Unauthenticated Download Date | 5/12/18 3:42 AM

show abstract

Identification of a 36‐kDa olive‐pollen allergen by in vitro and in vivo studies

Abstract: The 36-kDa protein constitutes a major allergen for olive-sensitized patients, but it is not equally represented in O. europaea pollens of different origins.

Cited by 20 publications

References 30 publications

Heat and chill requirements ofFraxinusflowering in Galicia (NW Spain)

Heat and chill requirements ofFraxinusflowering in Galicia (NW Spain)

Pollen from Different Olive Tree Cultivars Contains Varying Amounts of the Major Allergen Ole e 1

Hypersensitivity to Pollen of Olea Europea in Patients with Pollen Allergy in Zadar County, Croatia

Contact Info

Product

Resources

About