Identification of 6-methylsulfinylhexyl isothiocyanate as an apoptosis-inducing component in wasabi

Differentiation-associated alteration in sensitivity to apoptosis induced by (-)-epigallocatechin-3-O-gallate in HL-60 cells

“…2) representing characteristic features of apoptosis (6,16,18). Administration of the caspase inhibitor blocked the DNA fragmentation, confirming EGCG-mediated apoptosis in these cells (26). In vivo experiments also have shown that green tea polyphenols containing EGCG exhibited anti-tumor activity through induction of apoptosis (5,13).…”

“…The differentiated cells were then incubated with EGCG at various concentrations in the cell culture medium at 37°C for 24 h in the presence of each differentiation inducer, and compared with the results from undifferentiated cells. For detection of apoptotic bodies, HL-60 cells were incubated with 50 μM EGCG for 4 h and observed under a phase-contrast microscope as described previously (26).…”

“…DNA was isolated from HL-60 cells incubated with EGCG at various concentrations at 37°C for 24 h and electrophoresed in 2% agarose gel. After staining with SYBR Green I (Molecular Probes, Inc., OR, USA), degraded DNA was imaged using FluoroImager (Molecular Dynamics, Japan, Inc., Tokyo) as described previously (26). For confirmation of caspase-dependent DNA ladder formation, the pan-caspase inhibitor benzyloxycarbonyl-L-aspar-1-tyl)[(2,6-dichlorobenzoyl]oxy]methane (Z-Asp-CH 2 -DCB, Peptide Institute, Inc., Osaka) face 67LR is the target for EGCG and acts as the receptor for anti-tumor action of EGCG (23).…”

“…Employing human melanoma cell lines (A-375 amelanotic malignant melanoma and Hs-294T metastatic melanoma cells) and normal human epidermal melanocytes, it was demonstrated that EGCG caused a dose-dependent decrease in the viability and growth of both melanoma cell lines, whereas normal melanocytes were not affected (12). The cell line HL-60 was established from a patient with promyelocytic leukemia and is known to was added in the culture medium and incubated as described above (26).…”

Component Analysis of Wasabi Leaves and an Evaluation of their Anti-inflammatory Activity

“…The strong pungent smell associated with the roots of wasabi is derived directly from volatile allyl isothiocyanates via their reaction with myrosinase (Kojima et al, 1973). Several allyl isothiocyanate derivatives from wasabi have been reported to exhibit a variety of interesting biological properties, including antimicrobial (Inoue et al, 1983;Goi et al, 1985;Ono et al, 1998), antimutagenic (Morimitsu et al, 2000;Kinae et al, 2000), antiplatelet (Morimitsu et al, 2000) and apoptosis-inducing (Watanabe et al, 2003) activities. Notably, however, wasabi leaves are boiled or fried when they are used in foods, with the majority of the leaf being discarded.…”