Identification of 2H phosphoesterase superfamily proteins with 2′-CPDase activity

Mitsutomi, Shuhei; Akimitsu, Nobuyoshi; Sekimizu, Kazuhisa; Kaito, Chikara

doi:10.1016/j.biochi.2019.08.008

Cited by 3 publications

(2 citation statements)

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“…This specific chemistry has been observed in several prokaryotic species, and the 39-PDE activity (CNPase; EC 3.1.4.37) is well-known in many organisms, including humans. The observed 29-PDE activity, however, is relatively unprecedented in humans (39).…”

Section: Discussionmentioning

confidence: 94%

“…All chemicals were obtained from Sigma-Aldrich, were of the highest purity available, and were used without further purification. Nucleotides were obtained from the following sources: 29,39-NADcP, 29,39-cAMP, pApA, c-(ApGp), c [A (29,59) pG (29,59) p], c [A (29,59) pA (29,59) p], c [G (29,59) pA (39,59) p], and 29,39-cGAMP from BIOLOG Life Science Institute; cyclic adenosine diphosphate ribose and 39-NADP from Sigma-Aldrich; 29,39-cCMP from Santa Cruz Biotechnology; 39,59-cGMP, from Chem-Impex International Inc.; and 29-NADP from Alfa Aesar.…”

Section: Mouse Strains Cell Lines and Cdnasmentioning

confidence: 99%

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An unexpected 2-histidine phosphoesterase activity of suppressor of T-cell receptor signaling protein 1 contributes to the suppression of cell signaling

Yin

Frank

Zhou

et al. 2020

Journal of Biological Chemistry

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The suppressor of T-cell receptor (TCR) signaling (Sts) proteins Sts-1 and Sts-2 suppress receptor-mediated signaling pathways in various immune cells, including the TCR pathway in T cells and the Dectin-1 signaling pathway in phagocytes. As multidomain enzymes, they contain an N-terminal ubiquitin-association domain, a central Src homology 3 domain, and a C-terminal histidine phosphatase domain. Recently, a 2-histidine (2H) phosphoesterase motif was identified within the N-terminal portion of Sts. The 2H phosphoesterase motif defines an evolutionarily ancient protein domain present in several enzymes that hydrolyze cyclic phosphate bonds on different substrates, including cyclic nucleotides. It is characterized by two invariant histidine residues that play a critical role in catalytic activity. Consistent with its assignment as a phosphoesterase, we demonstrate here that the Sts-1 2H phosphoesterase domain displays catalytic, saturable phosphodiesterase activity toward the dinucleotide 2′,3′-cyclic NADP. The enzyme exhibited a high degree of substrate specificity and selectively generated the 3′-nucleotide as the sole product. Sts-1 also had phosphodiesterase catalytic activity toward a 5-mer RNA oligonucleotide containing a 2′,3′-cyclic phosphate group at its 3′ terminus. To investigate the functional significance of Sts-1 2H phosphoesterase activity, we generated His-to-Ala variants and examined their ability to negatively regulate cellular signaling pathways. Substitution of either conserved histidine compromised the ability of Sts-1 to suppress signaling pathways downstream of both the TCR and the Dectin-1 receptor. Our results identify a heretofore unknown cellular enzyme activity associated with Sts-1 and indicate that this catalytic activity is linked to specific cell-signaling outcomes.

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Section: Discussionmentioning

confidence: 94%