Identification of 2 Chinese Primary Pancreatic Ductal Adenocarcinoma Cancer Cell Lines and Their Phenotypes

Abstract

Objectives: Pancreatic cancer (PC) is one of the most lethal cancers. Currently, the most commonly used PC cell lines were all derived from White individuals, and few models can be used to study in Asian populations. In China, the incidence of PC is increasing every year, highlighting the need to develop new Chinese PC cell lines for cancer research.Methods: A total of 203 patients diagnosed with PC, enrolled from January 2012 to December 2017 in our hospital (Department of Hepatobiliary and Pancreatic Surgery), had received surgery. Primary PC patient tumor samples were harvested sterilely from surgery, minced, and digested in collagenase. Cells were cultured in plates precoated with L-polylysine.Results: Two primary PC cell lines, Si-Liang 187 and Si-Liang 188, were established, both of which grew as adherent monolayers and demonstrated distinguished phenotypes. Si-Liang 187 shows the typical mesenchymal pattern, whereas Si-Liang 188 shows epithelial phenotype. Moreover, they carry different genetic backgrounds according to whole exome sequencing.Conclusions: Two new Chinese PC cell lines were established, both of which were characterized and confirmed with high tumorigenicity. They may serve as useful tools for pathogenesis research when evaluating new treatment strategies in Asian patients.

Si-Liang 187 and Si-Liang 188, Two Recently Established Primary Pancreas Carcinoma Cell Lines, Are Crucial for Epithelial-to-Mesenchymal Transition

“…A recent study found that Ro60 levels are highly expressed in the nuclei of pancreatic cancer cells. 1 It was found that downregulation of Ro60 inhibited tumorigenesis in vivo, as well as proliferation, migration, and invasion of pancreatic ductal adenocarcinoma. 1 We describe a patient with pancreaticobiliary ampullary adenocarcinoma who 1 year before diagnosis was evaluated for autoimmune disease.…”

“…1 It was found that downregulation of Ro60 inhibited tumorigenesis in vivo, as well as proliferation, migration, and invasion of pancreatic ductal adenocarcinoma. 1 We describe a patient with pancreaticobiliary ampullary adenocarcinoma who 1 year before diagnosis was evaluated for autoimmune disease. Despite every other marker of autoimmunity being negative, the anti-Ro60 marker was extremely elevated at 564.9 chemiluminescent units.…”

“…7 The role of anti-Ro60 in solid tumor cancers has rarely been reported. 1 It is established that those with systemic autoimmune chronic inflammatory conditions, often diagnosed by a positive anti-Ro52 and anti-Ro60 blood test, have a higher frequency of malignancy. 8 In light of the patient's lack of autoimmune markers outside of anti-Ro60 in advance of diagnosis, it is possible that the increased anti-Ro60 may have been an early indicator of the patient's malignancy.…”

“…This is further supported by previous studies finding an upregulation of Ro60/SSA in pancreatic cancer tissues when compared with normal pancreatic tissue, Ro60 being highly expressed in the nuclei of pancreatic cancer cells, and the knockdown of Ro60 expression in tumor cells and mouse models inhibiting pancreatic cancer cell proliferation and overall tumor growth. 1 Because of Ro60 also promoting the invasive capacity of pancreatic cancer cells in wound healing and transwell invasion assays, an increased Ro60 in cancer patients may also indicate a more invasive presentation. 1 Downregulation of Ro60…”

“…W e read with interest the article regarding the identification of 2 new Chinese primary pancreatic ductal adenocarcinoma cancer cell lines. 1 Cancer cell lines are instrumental in oncologic research and are often used as the primary tools to investigate cancer mechanisms and develop appropriate and effective therapies. In the last decades, numerous human tumor type-specific cell lines have been applied as models for experimental cancer research in rare tumors and developmental biology.…”