Identification of 137Cs‐ and 241Am‐binding sites in the oyster crassostrea gigas

Milcent, M.C.; Goudard, F.; Durand, Jean-Pierre; Germain, Pierre; Pieri, J; George, Stephen

doi:10.1080/15216549600201141

Cited by 4 publications

(4 citation statements)

References 16 publications

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“…Cr, Co, Zn, Cd and 134 Cs were mainly found in the cytosolic fraction (30-87%) whereas 110m Ag and 241 Am were mainly associated with membranes and organelles (65-96%). These results are in agreement with those reported for other bivalves from temperate waters, e.g., the scallop Chlamys varia (Bustamante and Miramand in press) and the oyster Crassostrea gigas (Milcent et al 1996).…”

Section: Resultssupporting

confidence: 92%

Use of Radiotracer Techniques to Study Subcellular Distribution of Metals and Radionuclides in Bivalves from the Noumea Lagoon, New Calendonia

Métian

Hédouin

Barbot

et al. 2005

Bull Environ Contam Toxicol

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Section: Resultssupporting

confidence: 92%

Use of Radiotracer Techniques to Study Subcellular Distribution of Metals and Radionuclides in Bivalves from the Noumea Lagoon, New Calendonia

Métian

Hédouin

Barbot

et al. 2005

Bull Environ Contam Toxicol

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“…Pead et al 1995, and it has also been shown that transuranium elements such as Am, Cm, Cf and Pu are associated with the cytosolic ferritin in mammals and in marine invertebrates (Stover et al 1970, Bruenger et al 1976, Galey e t al. 1983, Goudard et al 1991, Milcent et al 1996. Thus, some authors have suggested that ferritin may be a general metal detoxicant protein carrier (Joshi & Zimmerman 1988, Joshi et al 1989, Muller et al 1991.…”

Section: Discussionmentioning

confidence: 99%

210Po binding to metallothioneins and ferritin in the liver of teleost marine fish

Durand¹,

Carvalho²,

Goudard³

et al. 1999

Mar. Ecol. Prog. Ser.

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The subcellular distribution of the naturally occurring radionuclide "' PO was investigated in the Liver of the Atlantic mackerel Scomber scombrus. The majority of the 2'0Po was found in the cytosol of the liver cells. Fractionation of the cytosol proteins by high performance size-exclusion and ion-exchange chromatography indicated that about 30% of 210Po was bound to ferritin and approximately 28% to metallothioneins. The affinity of 210Po for these proteins was confirmed by a similar binding of the artificial Z08Po isotope incubated in vitro with the cytosolic proteins. Two other proteins, likely selenium-and zinc-containing enzymes, may also bind smaller amounts of 210Po, about 8 % each. The extensive binding of 'lOPo to ferritin and metallothioneins is not accompanied by a similar strong binding of 21uPb, the radioactive grandparent of "OPo, which explains the generally very high 210Po:210Pb ratio observed in fish tissues and, in particular, fish liver KEY WORDS: Polonium. Fish. Metallothionein. Ferritin INTRODUCTIONPolonium-210, a high energy D-particle emitter in the uranium decay chain, is the major source of internal radiation dose to marine organisms (Cherry & Heyraud 1982, Carvalho 1988. This naturally occurring radionuclide is also responsible for a significant proportion of the radiation exposure of humans to background radiation, in particular, through consumption of seafood (Parfenov 1974, Hunt & Allington 1993, Yamamoto et al. 1994, Carvalho 1995a. 210Po is naturally occurring and ubiquitous in our environment, but, due to its radiotoxic properties, special attention has been paid to the possible enhancement of 210Po environmental concentrations which may occur from the inputs of effluents of phosphate-ore processing industries (Germain et al. 1992, Pennders et al. 1992, Carvalho 1995b, Swift et al. 1995.It is well known that 210Po is accumulated particularly by marine organisms and transferred through 'E-mail: jpdurandmnat svt sciences.univ-nantes.fr marine food chains (Heyraud & Cherry 1979, Carvalho 19881, with ingestion being the major route of entry (Carvalho & Fowler 1994). The tissues of some organisms, such as the hepatopancreas of crustaceans and fish liver may contain particularly high concentrations of 210Po. Furthermore, they display 210Po:2'0Pb ratios of 10' to 103 (Carvalho 1988), which correspond to an enhanced ratio in the food chain compared to the 210Po:210Pb ratio in sea water, which is around unity (Heyraud & Cherry 1979). The reason for an elevated 210Po:210Pb ratio in marine food chains is not known. Furthermore, several studies have suggested that the subcellular localization of 2'0Po, in particular in the cell nucleus, may have important implications with respect to the genetic radiotoxic effects of the 210Po U particles (Howell et al. 1990).Some attempts have been made previously to establish the subcellular localization of 2'0Po in tissues of marine invertebrates and small mammals. The aim of this study was to investigate the subcellular distribution of 210Po a...

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“…It can bind various metals on different types of sites: within the cavity, near the inner channel openings or the outside of apoferritin (Price & Joshi 1983, Pead et al 1995. Ferritin can also bind transuranium elements (Goudard et al 1991, Milcent et al 1996 Po is probably able to bind to these different sites present on ferritin and perhaps also to the iron core of the protein. Amino acid analysis showed very small amounts of cysteinyl residues in the ferritins of mackerel and oyster.…”

Section: Sephacryl S300 Fractionsmentioning

confidence: 99%

Ferritin and hemocyanin: 210Po molecular traps in marine fish, oyster and lobster

Durand¹,

Goudard²,

Barbot³

et al. 2002

Mar. Ecol. Prog. Ser.

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Identification of 137Cs‐ and 241Am‐binding sites in the oyster crassostrea gigas

Cited by 4 publications

References 16 publications

Use of Radiotracer Techniques to Study Subcellular Distribution of Metals and Radionuclides in Bivalves from the Noumea Lagoon, New Calendonia

Use of Radiotracer Techniques to Study Subcellular Distribution of Metals and Radionuclides in Bivalves from the Noumea Lagoon, New Calendonia

210Po binding to metallothioneins and ferritin in the liver of teleost marine fish

Ferritin and hemocyanin: 210Po molecular traps in marine fish, oyster and lobster

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