Identification of 1088 New Transposon Insertions of <i>Caenorhabditis elegans</i>: A Pilot Study Toward Large-Scale Screens

Martin, Edwige; Laloux, Hélène; Couette, Gaëlle; Alvarez, Thierry; Bessou, Catherine; Hauser, Oliver P.; Sookhareea, Satis; Labouesse, Michel; Ségalat, Laurent

doi:10.1093/genetics/162.1.521

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Cited by 26 publications

References 18 publications

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Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans

Linden

Plasterk

2004

Comp Funct Genom

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We present a strategy to identify and map large numbers of transposon insertions in the genome of Caenorhabditis elegans. Our approach makes use of the mutator strain mut-7, which has germline-transposition activity of the Tc1/mariner family of transposons, a display protocol to detect new transposon insertions, and the availability of the genomic sequence of C. elegans. From a pilot insertional mutagenesis screen, we have obtained 351 new Tc1 transposons inserted in or near 219 predicted C. elegans genes. The strategy presented provides an approach to isolate insertions of natural transposable elements in many C. elegans genes and to create a large-scale collection of C. elegans mutants.

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Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans

Linden

Plasterk

2004

Comp Funct Genom

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From genes to function: theC.elegansgenetic toolbox

Boulin

Hobert

2011

WIREs Developmental Biology

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Engineering the Caenorhabditis elegans Genome by Mos1-Induced Transgene-Instructed Gene Conversion

Robert

2012

Methods in Molecular Biology

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Mos1-induced transgene-instructed gene conversion (MosTIC) is a technique of choice to engineer the genome of the nematode Caenorhabditis elegans. MosTIC is initiated by the excision of Mos1, a DNA transposon of the Tc1/Mariner super family that can be mobilized in the germ line of C. elegans. Mos1 excision creates a DNA double-strand break that is repaired by several cellular mechanisms, including transgene-instructed gene conversion. For MosTIC, the transgenic repair template used by the gene conversion machinery is made of sequences that share DNA homologies with the genomic region to engineer and carries the modifications to be introduced in the genome. In this chapter, we present two MosTIC protocols routinely used.

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Identification of 1088 New Transposon Insertions of Caenorhabditis elegans: A Pilot Study Toward Large-Scale Screens

Abstract: We explored the feasibility of a strategy based on transposons to generate identified mutants of most Caenorhabditis elegans genes. A total of 1088 random new insertions of C. elegans transposons Tc1, Tc3, and Tc5 were identified by anchored PCR, some of which result in a mutant phenotype.

Cited by 26 publications

References 18 publications

Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans

Shotgun cloning of transposon insertions in the genome of Caenorhabditis elegans

From genes to function: theC.elegansgenetic toolbox

Engineering the Caenorhabditis elegans Genome by Mos1-Induced Transgene-Instructed Gene Conversion

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