Identification, mapping and evolutionary course of wheat lipoxygenase-1 genes located on the A genome

Garbus, Ingrid; Soresi, Daniela Soledad; Romero, José R.; Echenique, Viviana

doi:10.1016/j.jcs.2013.05.012

Cited by 11 publications

(16 citation statements)

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“…Hence, in the hexaploidy nature of common wheat (containing A, B and D subgenomes, which share *95 % sequence similarity), it is expected that probably more than 30 LOX copies could exist in common wheat genome. However, compared with such expectations, very few LOXs were mapped and isolated from common wheat so far (Feng et al 2010(Feng et al , 2012Garbus et al 2013). In addition, LOXs in subfamily II featured the presence of signal peptides, and their coding genes have only about 35 % overall sequence similarity with each other (Liavonchanka and Feussner 2006).…”

Section: Discussionmentioning

confidence: 98%

“…Recently, three LOX genes (TaLOX1, TaLOX2 and LOX3) from common wheat were isolated, and they were assigned to the short arm of chromosome 4D, the long arm of chromosome 5D, and chromosome 4A, respectively (Feng et al 2010(Feng et al , 2012. In durum wheat (Triticum turgidum, AABB), three copies of the LOX gene, Lpx-B1.1, Lpx-B1.2 and Lpx-B1.3, were identified in Lpx-B1 locus (Carrera et al 2007;Verlotta et al 2010), while only one LOX gene was found in Lpx-A1 locus of durum wheat (Garbus et al 2013). In addition, deletion of Lpx-B1.1 was found to be associated with reduction in LOX activity and improved pasta color (Carrera et al 2007).…”

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confidence: 99%

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Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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In higher plants, lipoxygenases (LOXs) catalyze the oxidation of polyunsaturated fatty acids and can accumulate to relatively high levels in the seeds. However, high LOX activity has often been found associated with harmful effects on the quality attributes and the longevity of harvested seeds. Here, we report the development and characterization of three homozygous T 5 transgenic lines (designated as LOX RNAi -4, 7 and 9, respectively) with LOX activity specifically reduced in the grains of common wheat. An RNAi hairpin directed under an endosperm-specific promoter was introduced into the genome of the elite wheat variety Longchun 23 using particle bombardment. Each transgenic line (LOX RNAi -4, 7 or 9) derived from separate integration event, which carried more than 20 copies of the transgene. The three LOX RNAi lines and wild-type (WT) Longchun 23 showed a similar level of LOX transcripts in the flag leaves. However, relative to WT control, LOX RNAi -4, 7 and 9 exhibited drastic decreases in the level of LOX transcripts during grain development compared with WT control. The LOX activity levels in the harvested grains of the three transgenic lines were significantly lower than that of WT control under either normal conditions or artificial aging (AA, stored at 40°C and 85 % humidity for 6 or 9 days) treatment. The whiteness values of the flour samples from LOX RNAi -4, 7 and 9 were generally and significantly higher than those of WT control regardless of the grains were stored under normal or AA conditions. Furthermore, the germination rates of LOX RNAi -4, 7 and 9 were substantially higher than those of WT control under AA treatment. Our data demonstrate that grainspecific reduction in LOX activity by RNAi may represent a generally applicable approach for improving the quality traits and longevity of crop seeds.Keywords Lipoxygenase Á RNA interference Á Germination rate Á Whiteness Á Wheat Zhenying Dong and Bo Feng have contributed equally to this work.Electronic supplementary material The online version of this article

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Section: Discussionmentioning

confidence: 98%

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confidence: 99%

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

et al. 2015

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“…a Sequences with identity to F. graminearum were not submitted to GenBank. Interestingly, copies for TDFs#5 and #50 located in the homologous group 4 reflected the inversion suffered by chromosome 4A (Devos et al 1995;Garbus et al 2013). In total, 28 TDFs were mapped to chromosome arms (Table 5).…”

Section: In Silico Mapping and Gene Isolationmentioning

confidence: 99%

Identification of genes induced by Fusarium graminearum inoculation in the resistant durum wheat line Langdon(Dic-3A)10 and the susceptible parental line Langdon

Soresi

Carrera

Echenique

et al. 2015

Microbiological Research

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The wheat recombinant chromosome inbred line LDN(Dic-3A)10, obtained through introgression of a Triticum dicoccoides disomic chromosome 3A fragment into Triticum turgidum spp. durum var. Langdon, is resistant to fusarium head blight (FHB) caused by Fusarium graminearum. To identify genes involved in FHB resistance, we used a cDNA-AFLP approach to compare gene expression between LDN(Dic-3A)10 and the susceptible parental line LDN at different time points post-inoculation. In total, 85 out of the ∼ 500 transcript-derived fragments (TDFs) were found to be differentially expressed: 36 and 19% were upregulated in LDN(Dic-3A)10 and LDN, respectively, whereas 45% were induced in both genotypes. Several of the cloned TDFs showed similarity to proteins involved in specific recognition of plant pathogens or associated with early responses to infection. Some TDFs specific to the inoculation response did not show similarity to characterized proteins. The availability of T. aestivum genome sequences allowed the in silico mapping of 28 TDFs and the acquirement of the corresponding gene sequences and, in some cases, their regulatory regions. Analysis of promoter regions revealed the potential existence of shared transcription regulation mechanisms. For instance, three TDF-associated genes contained binding sites for WRKY transcription factors, which have been implicated in the regulation of genes associated with pathogen defense, and three for abscisic acid-responsive element (ABRE). Collectively, our results revealed specific pathogen recognition in the interactions of LDN and LDN(Dic-3A)10 with F. graminearum. Such recognition leads to changes in the expression of several transcripts, attributable to the presence of the wheat QTL Qfhs.ndsu-3AS.

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“…Given the rapid progress of modern molecular and biological techniques, many Lox genes have been mapped and cloned in cereal crops, including barley (Hordeum vulgare L.) (van Mechelen et al 1999), durum wheat (Triticum turgidum L.) (Carrera et al 2007;Garbus et al 2009;Verlotta et al 2010), and bread wheat (Triticum aestivum L.) (Feng et al 2010(Feng et al , 2012Geng et al 2012;Garbus et al 2013). To date, partial or complete sequences of eight Lox genes, namely, Lpx-A3 (DQ474242 and DQ474244),…”

Section: Introductionmentioning

confidence: 99%

“…Three alleles have also been identified for Lpx-B1.1, Lpx-B1.1a (HM126466), Lpx-B1.1b (HM126468), and Lpx-B1.1c (HM126470) (Verlotta et al 2010). Four Lpx sequences have been identified and annotated in T. aestivum genome, including Lpx pseudogene in the A genome (Lpx-A1 -like; KC679303), LpxB1.1 a (KC679300) and LpxB1.2 (KC679301) genes in the B genome, and Lpx-D1 (KC679302) in the D genome (Garbus et al 2009(Garbus et al , 2013. Feng et al (2010Feng et al ( , 2012 cloned the gDNA or cDNA sequences of three Lox genes, i.e., TaLox1 (GU167920/ GQ166692), TaLox2 (GU167921/GQ166691), and TaLox3 (HQ913602).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of lipoxygenase genes on chromosome 4BS in Chinese bread wheat (Triticum aestivum L.)

Zhang¹,

Chen

Wu³

et al. 2015

Theor Appl Genet

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This study cloned two novel TaLox genes on chromosome of 4BS and developed a co-dominant marker, Lox-B23, in bread wheat that showed highly significant association with lipoxygenase activity. Lipoxygenase (Lox), a critical enzyme in the carotenoid biosynthetic pathway, significantly influences the color and processing quality of wheat-based products. Two novel Lox genes, designated TaLox-B2 and TaLox-B3, were cloned on chromosome 4BS of Chinese bread wheat. The deduced amino acid sequence showed that both TaLox-B2 and TaLox-B3 genes encoded an 861-aa protein and possessed a lipoxygenase superfamily domain at the 170-838 interval. Two different TaLox-B2 alleles, designated TaLox-B2a and TaLox-B2b, were subsequently discovered. A co-dominant marker, Lox-B23, was developed based on sequences of TaLox-B2a, TaLox-B2b, and TaLox-B3 genes to precisely distinguish these three alleles in Chinese bread cultivars. Among five allelic combinations of Lox genes at Lox-B1, Lox-B2, and Lox-B3 loci, wheat cultivars with TaLox-B1a/TaLox-B2a/TaLox-B3a combination exhibited the highest Lox activity, whereas those with TaLox-B1a/TaLox-B2b/TaLox-B3b combination significantly showed the lowest Lox activity. A RIL population was used to evaluate the influence of TaLox-B3a gene on Lox activity. Results showed that TaLox-B3a gene could significantly increase the Lox activity in bread wheat. Physical mapping indicated that both TaLox-B2 and TaLox-B3 genes were located on chromosome 4BS in bread wheat. This study provides useful information to further understand the molecular and genetic bases of Lox activity in bread wheat.

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Identification, mapping and evolutionary course of wheat lipoxygenase-1 genes located on the A genome

Cited by 11 publications

References 30 publications

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Grain-specific reduction in lipoxygenase activity improves flour color quality and seed longevity in common wheat

Identification of genes induced by Fusarium graminearum inoculation in the resistant durum wheat line Langdon(Dic-3A)10 and the susceptible parental line Langdon

Molecular characterization of lipoxygenase genes on chromosome 4BS in Chinese bread wheat (Triticum aestivum L.)

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