Identification, in silico characterization, and expression analysis of NBS-LRR class of R genes against stem and crown rot disease in Trifolium alexandrinum L.

Abstract

R genes with colossal contributions to disease resistance possess conserved domains with confounded roles. Based on the conserved domains, R genes encoded proteins are classified. The members of nucleotide-binding site, NBS-Leucine rich repeats, LRR class of R genes are significant candidates involved in plant disease-resistance pathways. In this study, the genetic elements contributing to stem and crown rot disease resistance were explored through transcriptome probing based on conserved NBS domain under disease stress in berseem clover. Expression analysis of identified gene sequences revealed their upregulation during stem and crown rot disease. The in silico characterization imparted strong support towards the predictive role of identified RGAs in disease resistance.

Selection and validation of reference genes for normalizing qRT‒PCR gene expression studies in Colletotrichum gloeosporioides and interaction with the guava plants

“…However, the reliability of the results depends upon the reliable reference genes vis-à-vis their expression stability for normalizing expression data (Huang et al, 2018) that should not be altered under varied experimental conditions (Melgar-Rojas et al, 2015;Ijaz et al, 2020b). Under varying experimental conditions, it is ambiguous to use traditional reference genes for normalizing target gene expression without prior investigation of their stability (Galli et al, 2015;Nasir et al, 2023).…”

In silico structural-functional characterization of three differentially expressed resistance gene analogs identified in Dalbergia sissoo against dieback disease reveals their role in immune response regulation

“…The non-TIRs are attributed to the coiled-coil (CC) domain and the less common leucine zipper (LZ) domain ( Babar et al., 2021 ). The NBS domain is approximately 300 amino acids in length with four subdomains, kinase 1a (P-loop), kinase 2, kinase 3a, and a hydrophobic GLPAL, which are involved in ATP/GTP hydrolyses and work as molecular switches in signal transduction network ( Nasir et al., 2023 ). The LRR domain has approximately 10-40 leucine-rich-repeat motifs specified for pathogen recognition ( Cannon et al., 2002 ).…”