Identification, Growth Profile and Probiotic Properties of Autochthonous Intestinal Bacteria of Sagor catfish (&lt;i&gt;Hexanematichthys sagor&lt;/i&gt;)

Curdlan, a β-1,3/1,6-glucan found in Alcaligenes faecalis (A. faecalis) wall, activates innate and humoral immunity. The aim of this study is to evaluate whether pretreated rats with A. faecalis A12C could prevent sepsis disturbances and identify the immunomodulatory mechanisms involved. Experiments occurred in two stages: a survival study with 16 rats randomly divided into septic (SC) (n = 8) and septic pretreated (SA) (n = 8) groups and 45 rats divided into four groups: healthy (AGUSAN) (n = 9), septic (AGUIC) (n = 13), septic pretreated (AGUIA) (n = 14), and healthy pretreated (AGUSTO) (n = 9). Sepsis was induced by cecal ligation and puncture after 30 days of A. faecalis A12C pretreatment or without. SA group had a higher survival rate of 58% vs. 16% for SC group (P < 0.05). Overall, AGUIA showed better status than AGUIC (P < 0.01). Higher monocytosis was found in AGUIA and AGUSTO vs. AGUIC and AGUSAN, respectively (P < 0.05). A gradual increase in curdlan fecal concentration was observed in AGUIA during pretreatment. Fecal concentrations of Escherichia coli significantly decreased in AGUIA and AGUSTO. Bacterial load in urine, peritoneal lavage fluid (PLF), and bronchoalveolar lavage fluid (BALF) decreased (P < 0.05) in AGUIA vs. AGUIC. Finally, lower inflammation was observed in serum, BALF, and PLF, with reduced IL-6, IL-10, IL-1β, and TNF-α, along with less damage in lungs and peritoneum in AGUIA vs. AGUIC. These findings suggest the connection between curdlan—produced by A. faecalis A12C—with the immune system and the reduction in severity of experimental sepsis.

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Isolation and characterization of dye-degrading bacteria from batik wastewater

Rakhmania

Ibrahim

Abba

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Azo dye is typically dye used in batik industries that is toxic to aquatic organisms and humans. Chemical methods can effectively degrade the dye effluent however they produce more toxic compounds. Biological methods offer an alternative that is more environmentally friendly and produce readily degradable compounds. Dye degrading bacteria can degrade the dyes in the effluent under anaerobic for decolorization followed by aerobic condition to produce readily degradable compounds. The batik wastewater sample was collected in Kelantan, Malaysia and was analyzed for COD, pH, and color. The Horikoshi-II media pH 10 was used to propagate growth of bacteria in the dye effluent. The dye degrading bacteria then was isolated from the dye effluent using serial dilution spread plate and streak plate method; One bacterium was finally selected based on faster growth. The specific growth rate and generation time of isolated bacteria were 0.069 h-1 and 14 h respectively. The isolated bacteria have characteristic producing red pigment and gram-positive bacilli. Overall reduction of color and COD by isolated bacteria were 23% and 41% respectively. The isolated bacteria were identified as Bacillus sp. strain ARNZ2707 via 16S rRNA sequence analysis.

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Identification, Growth Profile and Probiotic Properties of Autochthonous Intestinal Bacteria of Sagor catfish (<i>Hexanematichthys sagor</i>)

Cited by 4 publications

References 29 publications

Methods for Isolating and Identifying Probiotic Bacteria from Fishes

Methods for Isolating and Identifying Probiotic Bacteria from Fishes

Long-Term Treatment with Alcaligenes faecalis A12C Improves Host Resistance to Pathogens in Septic Rats: Possible Contribution of Curdlan-Like Immune Trainer

Isolation and characterization of dye-degrading bacteria from batik wastewater

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