Identification, genetic characterization and virulence of Serbian Erwinia amylovora isolates

Popović, Tatjana; Jelušić, Aleksandra; Živković, Ljiljana; Živković, Natalija; Iličić, Renata; Stanisavljević, Rade; Stanković, Slaviša

doi:10.1007/s10658-020-02046-1

Cited by 6 publications

(6 citation statements)

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“…The results obtained in this work in the identification of E. amylovora isolates using classical and molecular methods concur with those reported by other authors (Gavrilović, 2010;Gavrilović et al, 2008;Radunović, 2018;Popović et al, 2020). Based on the findings pertaining to the morphological and biochemical characteristics, pathogenicity, and identification achieved using molecular methods, it was concluded that the collar and rootstock blight symptoms causing young apple tree decay in Serbia were caused by the bacterium E. amylovora.…”

Section: Resultssupporting

confidence: 92%

“…The plant pathogenic bacterium Erwinia amylovora, the causative agent of fire blight on pome fruits, is widespread in Europe, Asia, North Africa, the Eastern Mediterranean, North and Central America and New Zealand (Bonn and van der Zwet, 2000;Van der Zwet, 2002;CABI/EPPO, 2007;OEPP/EPPO, 2013). This bacterium has been described to be pathogenic for 200 plant species, mainly affecting those within the Rosaceae family, but also the genera Chaenomeles, Cotoneaster, Crataegus, Cydonia, Eriobotria, Malus, Mespilus, Pyrus, Photinia, Pyracantha, Sorbus, and Stranvaesia. However, the most extensive losses have been recorded on pear, apple and quince cultivars in many countries including Serbia (Popović et al, 2020). E. amylovora has been described on raspberries (Starr et al, 1951;Atanasova et al, 2005), Japanese and European plums (Vanneste et al, 2002), hokeberry (Aronia melanocarpa) (Atanasova et al, 2005), Spirea prunifolia, Rosa canina and R. rugosa (Bastas et al, 2013;Bastas and Sahin, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The typical symptoms caused by E. amylovora include blossom, fruit, shoot, perennial branch, limb and trunk blight (Popović et al, 2020). The most specific and common symptom in Serbian conditions is shoot blight and bending in the form of a "shepherd's crook" during summer months (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

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Survey of apple collar and rootstock blight in Serbia

Iličić¹,

Popović²

2021

Acta agriculturae Serbica

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Apple production in Serbia is characterized by the introduction of new technologies and the establishment of intensive plantations based on modern systems. In intensive apple production systems, sudden and rapid decay of young apple trees exhibiting collar and rootstock blight symptoms in the first few years after planting is becoming a prevalent issue. However, knip or nursery trees with lateral branches (feathers) on rootstock M.9 i.e. oneto five-year old are most seriously affected. Based on a longitudinal survey of young apple plantations in Serbia and the results of pathogen detection and identification using conventional and molecular methods, the plant pathogenic bacterium Erwinia amylovora was found as the causative agent. By increasing the incidence of collar and rootstock blight in young apple trees, E. amylovora is gaining importance as the most destructive disease in apple production. In order to control E. amylovora effectively and apply appropriate control measures, it is necessary to survey the development of lesser-known symptoms caused by this pathogen, which are becoming prevalent in Serbian agroecological conditions.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Survey of apple collar and rootstock blight in Serbia

Iličić¹,

Popović²

2021

Acta agriculturae Serbica

Self Cite

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“…To understand this dissemination some studies genetically characterized the E. amylovora strains epidemiology at local, national, and regional levels ( Halupecki et al, 2006 ; Végh et al, 2012 ; Rhouma et al, 2014 ; Doolotkeldieva et al, 2019 ; Popović et al, 2020 ). In particular, the multi locus sequencing analysis (MLSA) provided phylogenetic studies of E. amylovora ( Facey et al, 2015 ; Doolotkeldieva et al, 2019 ), and of other phytopathogenic bacteria ( Wicker et al, 2012 ; Flores et al, 2018 ; Martins et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Variability within a clonal population of Erwinia amylovora disclosed by phenotypic analysis

Mendes

Amaro

Luz

et al. 2022

PeerJ

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Background Fire blight is a destructive disease of pome trees, caused by Erwinia amylovora, leading to high losses of chain-of-values fruits. Major outbreaks were registered between 2010 and 2017 in Portugal, and the first molecular epidemiological characterization of those isolates disclosed a clonal population with different levels of virulence and susceptibility to antimicrobial peptides. Methods This work aimed to further disclose the genetic characterization and unveil the phenotypic diversity of this E. amylovora population, resorting to MLSA, growth kinetics, biochemical characterization, and antibiotic susceptibility. Results While MLSA further confirmed the genetic clonality of those isolates, several phenotypic differences were recorded regarding their growth, carbon sources preferences, and chemical susceptibility to several antibiotics, disclosing a heterogeneous population. Principal component analysis regarding the phenotypic traits allows to separate the strains Ea 630 and Ea 680 from the remaining. Discussion Regardless the genetic clonality of these E. amylovora strains isolated from fire blight outbreaks, the phenotypic characterization evidenced a population diversity beyond the genotype clonality inferred by MLSA and CRISPR, suggesting that distinct sources or environmental adaptations of this pathogen may have occurred. Conclusion Attending the characteristic clonality of E. amylovora species, the data gathered here emphasizes the importance of phenotypic assessment of E. amylovora isolates to better understand their epidemiological behavior, namely by improving source tracking, make risk assessment analysis, and determine strain-specific environmental adaptations, that might ultimately lead to prevent new outbreaks.

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“…The etiological agent responsible for this disease is the quarantine bacterium Erwinia amylovora (Burril) Winslow et al, a Gram-negative of the Erwiniaceae family, which has been considered as one of the top ten plant pathogenic bacteria [4,5]. This pathogen was first reported in North America, but is currently spread to Europe (United Kingdom, Spain, France, Italy, Hungary, and Portugal), South Korea, New Zealand, and the Middle East [6][7][8][9][10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Five Antimicrobial Peptides against the Plant Pathogen Erwinia amylovora

et al. 2021

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Fire blight is a major pome fruit trees disease that is caused by the quarantine phytopathogenic Erwinia amylovora, leading to major losses, namely, in pear and apple productions. Nevertheless, no effective sustainable control treatments and measures have yet been disclosed. In that regard, antimicrobial peptides (AMPs) have been proposed as an alternative biomolecule against pathogens but some of those AMPs have yet to be tested against E. amylovora. In this study, the potential of five AMPs (RW-BP100, CA-M, 3.1, D4E1, and Dhvar-5) together with BP100, were assessed to control E. amylovora. Antibiograms, minimal inhibitory, and bactericidal concentrations (minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), growth and IC50 were determined and membrane permeabilization capacity was evaluated by flow cytometry analysis and colony-forming units (CFUs) plate counting. For the tested AMPs, the higher inhibitory and bactericidal capacity was observed for RW-BP100 and CA-M (5 and 5–8 µM, respectively for both MIC and MBC), whilst for IC50 RW-BP100 presented higher efficiency (2.8 to 3.5 µM). Growth curves for the first concentrations bellow MIC showed that these AMPs delayed E. amylovora growth. Flow cytometry disclosed faster membrane permeabilization for CA-M. These results highlight the potential of RW-BP100 and CA-M AMPs as sustainable control measures against E. amylovora.

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Identification, genetic characterization and virulence of Serbian Erwinia amylovora isolates

Cited by 6 publications

References 38 publications

Survey of apple collar and rootstock blight in Serbia

Survey of apple collar and rootstock blight in Serbia

Variability within a clonal population of Erwinia amylovora disclosed by phenotypic analysis

In Vitro Evaluation of Five Antimicrobial Peptides against the Plant Pathogen Erwinia amylovora

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