Identification, functional characterization and phylogenetic analysis of double stranded RNA degrading enzymes present in the gut of the desert locust, Schistocerca gregaria

Wynant, Niels; Santos, Dulce; Verdonck, Rik; Spit, Jornt; Wielendaele, Pieter Van; Broeck, Jozef Vanden

doi:10.1016/j.ibmb.2013.12.008

Cited by 139 publications

(177 citation statements)

References 24 publications

Supporting

Mentioning

156

Contrasting

Unclassified

Order By: Relevance

“…For a multiple sequence alignment, two dsRNase sequences from Tribolium castaneum and one from Locusta migratoria were retrieved through BLAST searches against public databases on the NCBI website. In addition, Sg_dsRNase1 and Bm_dsRNase1 were selected from S. gregaria and B. mori based on previously published research (Liu et al, 2012;Wynant et al, 2014b).…”

Section: In Silico Detection and Phylogenymentioning

confidence: 99%

“…Yet, in several insect species, injection of naked dsRNA into the body cavity can successfully trigger RNAi, while feeding of the same dsRNA is less efficient, or completely ineffective. This was for instance observed in locusts, which are highly sensitive to injected dsRNA, while orally delivered dsRNA does not induce an efficient RNAi response (Luo et al, 2013;Wynant et al, 2014b).…”

Section: Introductionmentioning

confidence: 99%

“…In the digestive juice of larvae of the silkworm, Bombyx mori, an alkaline nuclease that could digest dsRNA has been isolated (Arimatsu et al, 2007). Later, high nuclease activity also has been demonstrated in the gut juice of locusts (Luo et al, 2013;Wynant et al, 2014b), as well as in salivary secretions of the pea aphid, Acyrthosiphon pisum (Christiaens et al, 2014), and the tarnished plant bug, Lygus lineolarus (Allen and Walker, 2012), to name a few. It is therefore very plausible that dsRNA degradation in the alimentary tract of insects contributes to a lowered RNAi efficiency upon feeding on dsRNA.…”

Section: Introductionmentioning

confidence: 99%

“…Finally, we tried a similar approach in the desert locust, Schistocerca gregaria, an infamous pest insect, capable of forming huge devastating swarms when in the gregarious phase (Verlinden et al, 2009). Four nuclease genes were previously characterized in this species (Wynant et al, 2014b). Here, they were knocked down simultaneously in an effort to improve the RNAi sensitivity upon feeding of dsRNA.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata , but not in the desert locust, Schistocerca gregaria

Spit

Philips

Wynant

et al. 2017

Insect Biochemistry and Molecular Biology

Self Cite

133

141

View full text Add to dashboard Cite

Abstract:The responsiveness towards orally delivered dsRNA and the potency of a subsequent environmental RNA interference (RNAi) response strongly differs between different insect species. While some species are very sensitive to dsRNA delivery through the diet, others are not. The underlying reasons for this may vary, but degradation of dsRNA by nucleases in the gut lumen is believed to play a crucial role. The Colorado potato beetle, Leptinotarsa decemlineata, is a voracious defoliator of potato crops worldwide, and is currently under investigation for novel control methods based on dsRNA treatments. Here we describe the identification and characterization of two nuclease genes exclusively expressed in the gut of this pest species. Removal of nuclease activity in adults increased the sensitivity towards dsRNA and resulted in improved protection of potato plants. A similar strategy in the desert locust, Schistocerca gregaria, for which we show a far more potent nuclease activity in the gut juice, did however not lead to an improvement of the RNAi response.Possible reasons for this are discussed. Taken together, the present data confirm a negative effect of nucleases in the gut on the environmental RNAi response, and further suggest that interfering with this activity is a strategy worth pursuing for improving RNAi efficacy in insect pest control applications.

show abstract

Section: In Silico Detection and Phylogenymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata , but not in the desert locust, Schistocerca gregaria

Spit

Philips

Wynant

et al. 2017

Insect Biochemistry and Molecular Biology

Self Cite

133

141

View full text Add to dashboard Cite

show abstract

“…49 However, dsRNAs are susceptible to breakdown like all macromolecules and nuclease activity has been characterized in multiple regions of the digestive tract of various insects. For example nuclease activity against dsRNA has been found in the saliva of the tarnished plant bug 50 and pea aphid 51 as well as the midgut of the silk worm, 52 the desert locust, 53 and others. 54 It is important to note that the digestive tract tissue architecture and environment are quite divergent within invertebrates, 55,56 leading to differences in potential for sRNA degradation.…”

Section: Introductionmentioning

confidence: 99%

Uptake and impact of natural diet-derived small RNA in invertebrates: Implications for ecology and agriculture

Chan

Snow

2016

RNA Biology

View full text Add to dashboard Cite

The putative transfer and gene regulatory activities of diet-derived small RNAs (sRNAs) in ingesting animals are still debated. The existence of natural uptake of diet-derived sRNA by invertebrate species could have significant implication for our understanding of ecological relationships and could synergize with efforts to use RNA interference (RNAi) technology in agriculture. Here, we synthesize information gathered from studies in invertebrates using natural or artificial dietary delivery of sRNA and from studies of sRNA in vertebrate animals and plants to review our current understanding of uptake and impact of natural dietderived sRNA on invertebrates. Our understanding has been influenced and sometimes confounded by the diversity of invertebrates and ingested plants studied, our limited insights into how gene expression may be modulated by dietary sRNAs at the mechanistic level, and the paucity of studies focusing directly on natural uptake of sRNA. As such, we suggest 2 strategies to investigate this phenomenon more comprehensively and thus facilitate the realization of its potentially broad impact on ecology and agriculture in the future.

show abstract

Development of RNAi methods to control the harlequin bug, Murgantia histrionica

Howell

Mogilicherla

Gurusamy

et al. 2020

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The harlequin bug (HB), Murgantia histrionica, is a major pest of cabbage family plants throughout its range in the United States. RNA interference (RNAi) is a posttranscriptional gene silencing mechanism that is showing promise as a biopesticide due to the ability to target species-specific genes necessary for growth and/or survival with synthetic double-stranded RNA (dsRNA). In the present study, dsRNA stability assays

show abstract

Identification, functional characterization and phylogenetic analysis of double stranded RNA degrading enzymes present in the gut of the desert locust, Schistocerca gregaria

Cited by 139 publications

References 24 publications

Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata , but not in the desert locust, Schistocerca gregaria

Knockdown of nuclease activity in the gut enhances RNAi efficiency in the Colorado potato beetle, Leptinotarsa decemlineata , but not in the desert locust, Schistocerca gregaria

Uptake and impact of natural diet-derived small RNA in invertebrates: Implications for ecology and agriculture

Development of RNAi methods to control the harlequin bug, Murgantia histrionica

Contact Info

Product

Resources

About