Identification, expression pattern, and feature analysis of cuticular protein genes in the pine moth Dendrolimus punctatus (Lepidoptera: Lasiocampidae)

Yang, Chungang; Yang, Pengcheng; Zhang, Sufang; Shi, Zhiyong; Kang, Le; Zhang, Aibing

doi:10.1016/j.ibmb.2017.03.003

Cited by 38 publications

(33 citation statements)

References 73 publications

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“…The number of CPs is similar to that found in other Hymenoptera species, such as A. mellifera (45 CPs) (Consortium, 2006;Micas et al, 2016) and N. vitripennis (76 CPs) (Werren et al, 2010;Willis, 2010). However, the number of CPs in M. mediator is lower than some non-Hymenoptera species (Dittmer et al, 2015;Yang et al, 2017). The low number of annotated CPs in Hymenoptera species could be explained by their highly developed social organization (A. mellifera) or parasitic lifestyle during early developmental stages (N. vitripennis).…”

Section: Discussionsupporting

confidence: 77%

“…Analysis of insect genomes showed that around 1% of protein-coding genes are represented by CP (Ioannidou et al, 2014). CPs have been identified and analyzed on genomic, transcriptomic, or proteomic levels in more than 20 insect species (Shofuda et al, 1999;Adams et al, 2000;Consortium, 2006;He et al, 2007;Togawa et al, 2007;Futahashi et al, 2008;Cornman & Willis, 2009;Gallot et al, 2010;Liang et al, 2010;Werren et al, 2010;Carrasco et al, 2011;Suetsugu et al, 2013;Sadd et al, 2015;Micas et al, 2016;Jan et al, 2017;Yang et al, 2017;Zhao et al, 2017;Zhou et al, 2017;Pan et al, 2018). However, research related to CPs has mostly focused on Diptera and Lepidoptera.…”

Section: Introductionmentioning

confidence: 99%

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Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

Volovych

Zhe

et al. 2019

Insect Science

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Recently, parasitoid wasp species Microplitis mediator has evoked increasing research attention due to its possible use in the control of Lepidoptera insects. Because insect development involves changes in cuticle composition, identification and expression analysis of M. mediator cuticular proteins may clarify the mechanisms involved in parasite development processes. We found 70 cuticular proteins from the M. mediator transcriptome and divided them into seven distinct families. Expression profiling indicated that most of these cuticular protein genes have expression peaks specific for one particular developmental stage of M. mediator. Eggs and pupae have the highest number of transcriptionally active cuticular protein genes (47 and 52 respectively). Only 12 of these genes maintained high expression activity during late larval development. Functional analysis of two larval proteins, MmCPR3 and MmCPR14, suggested their important role in the proper organization of the cuticle layers of larvae. During M. mediator larval development, normal cuticle formation can be supported by a limited number of cuticular proteins.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

Volovych

Zhe

et al. 2019

Insect Science

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“…In addition to these well-identified structural CPs, our computational approach is based on both low complexity and specific compositional skewness (i.e., compositional similarity to cognate CPs), which allowed us to identify new CPs that were already ''randomly'' picked-up by analytical works in some other insect models, such as Bombyx mori (Dong et al, 2016), Manduca sexta (Tetreau et al, 2015a(Tetreau et al, , 2015b, Anopheles gambiae (Zhou et al, 2016(Zhou et al, , 2017, and Dendrolimus punctatus (Yang et al, 2017). The most recently identified family of CPs was the CPAP1/3 family (Pan et al, 2018), but all other transcripts or proteins identified individually from -omic analyses of insect cuticles were classified as ''ungrouped CPs'' (NlGp21.92 in N. lugens (Lu et al, 2018)) or as poorly defined low-complexity classes.…”

Section: Glands Expression Profiles and The Spatiotemporal Limitmentioning

confidence: 99%

Insect Mouthpart Transcriptome Unveils Extension of Cuticular Protein Repertoire and Complex Organization

Guschinskaya

Ressnikoff

Arafah³

et al. 2020

iScience

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“…To understand the evolutionary history of BmorCPH24, we performed phylogenetic analysis using the genes encoding cuticular protein with low complexity sequence from four different insect species, B. mori , Dendrolimus punctatus (Yang et al 2017), Manduca sexta (Dittmer et al 2015), and Anopheles gambiae (Cornman and Willis 2009). All proteins were aligned using Muscle.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Body Shape and Coloration of Silkworm Larvae Are Influenced by a Novel Cuticular Protein

et al. 2017

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The genetic basis of body shape and coloration patterns on caterpillars is often assumed to be regulated separately, but it is possible that common molecules affect both types of trait simultaneously. Here we examine the genetic basis of a spontaneous cuticle defect in silkworm, where larvae exhibit a bamboo-like body shape and decreased pigmentation. We performed linkage mapping and mutation screening to determine the gene product that affects body shape and coloration simultaneously. In these mutant larvae we identified a null mutation in , a gene encoding a cuticular protein with low complexity sequence. Spatiotemporal expression analyses showed that is expressed in the larval epidermis postecdysis. RNAi-mediated knockdown and CRISPR/Cas9-mediated knockout of produced the abnormal body shape and the inhibited pigment typical of the mutant phenotype. In addition, our results showed that may be involved in the synthesis of endocuticle and its disruption-induced apoptosis of epidermal cells that accompanied the reduced expression of R&R-type larval cuticle protein genes and pigmentation gene Strikingly,, a fast-evolving gene, has evolved a new function responsible for the assembly of silkworm larval cuticle and has evolved to be an indispensable factor maintaining the larval body shape and its coloration pattern. This is the first study to identify a molecule whose pleiotropic function affects the development of body shape and color patterns in insect larvae.

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Identification, expression pattern, and feature analysis of cuticular protein genes in the pine moth Dendrolimus punctatus (Lepidoptera: Lasiocampidae)

Cited by 38 publications

References 73 publications

Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

Identification and temporal expression profiles of cuticular proteins in the endoparasitoid wasp, Microplitis mediator

Insect Mouthpart Transcriptome Unveils Extension of Cuticular Protein Repertoire and Complex Organization

Body Shape and Coloration of Silkworm Larvae Are Influenced by a Novel Cuticular Protein

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