Identification, Expression and IAA-Amide Synthetase Activity Analysis of Gretchen Hagen 3 in Papaya Fruit (Carica papaya L.) during Postharvest Process

Liu, Kaidong; Wang, Jinxiang; Li, Haili; Zhong, Jun; Feng, Shaoxian; Pan, Yaoliang; Yuan, Changchun

doi:10.3389/fpls.2016.01555

Cited by 17 publications

(19 citation statements)

References 57 publications

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“…To explore the evolutionary relationships of the GH3 genes from the three species of Brassica and A. thaliana , a phylogenetic tree was generated with 19 A. thaliana , 38 B. rapa , 25 B. oleracea , and 66 B. napus members, which would contribute to understanding the potential roles of the GH3 genes in Brassica species. The phylogenetic tree was divided into three groups (I, II, and III), and this pattern of three groups for the GH3 genes in the phylogenetic tree was in line with previous reports [23,24,46]. Group III possessed the highest number of the GH3 members (Figure 3).…”

Section: Resultssupporting

confidence: 88%

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The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors

Wang

et al. 2019

Genes

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The GH3 gene family plays a vital role in the phytohormone-related growth and developmental processes. The effects of allopolyploidization on GH3 gene structures and expression levels have not been reported. In this study, a total of 38, 25, and 66 GH3 genes were identified in Brassica rapa (ArAr), Brassica oleracea (CoCo), and Brassica napus (AnACnCn), respectively. BnaGH3 genes were unevenly distributed on chromosomes with 39 on An and 27 on Cn, in which six BnaGH3 genes may appear as new genes. The whole genome triplication allowed the GH3 gene family to expand in diploid ancestors, and allopolyploidization made the GH3 gene family re-expand in B. napus. For most BnaGH3 genes, the exon-intron compositions were similar to diploid ancestors, while the cis-element distributions were obviously different from its ancestors. After allopolyploidization, the expression patterns of GH3 genes from ancestor species changed greatly in B. napus, and the orthologous gene pairs between An/Ar and Cn/Co had diverged expression patterns across four tissues. Our study provides a comprehensive analysis of the GH3 gene family in B. napus, and these results could contribute to identifying genes with vital roles in phytohormone-related growth and developmental processes.

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Section: Resultssupporting

confidence: 88%

“…The GH3 proteins play important roles in plant development and light signal pathways [18,19,20,21]. So far, the identification and analysis of the GH3 gene family have been performed in different plant species, such as Arabidopsis thaliana , rice, maize, Medicago truncatula , Carica papaya , and so on [12,17,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors

Wang

et al. 2019

Genes

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“…These ethylene-related genes may play important roles in the abnormal softening and coloration that occur in papaya fruit upon chilling 31 . Many works have demonstrated that GH3, ARF, and Aux/IAA protein families are also involved in papaya fruit postharvest processes 20 , 23 , 32 . In our study, at least one crotonylation site in each ethylene- (ACO1/2/3 and ACS) and auxin (ARF, AUX1, TIR and GH3)-related enzyme has been identified.…”

Section: Discussionmentioning

confidence: 99%

A qualitative proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.)

Liu

Yuan

et al. 2018

Sci Rep

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Lysine crotonylation of histone proteins is a recently-identified post-translational modification with multiple cellular functions. However, no information about lysine crotonylation of non-histone proteins in fruit cells is available. Using high-resolution LC-MS/MS coupled with highly sensitive immune-affinity antibody analysis, a global crotonylation proteome analysis of papaya fruit (Carica papaya L.) was performed. In total, 2,120 proteins with 5,995 lysine crotonylation sites were discovered, among which eight conserved motifs were identified. Bioinformatic analysis linked crotonylated proteins to multiple metabolic pathways, including biosynthesis of antibiotics, carbon metabolism, biosynthesis of amino acids, and glycolysis. particularly, 40 crotonylated enzymes involved in various pathways of amino acid metabolism were identified, suggesting a potential conserved function for crotonylation in the regulation of amino acid metabolism. Numerous crotonylation sites were identified in proteins involved in the hormone signaling and cell wall-related pathways. Our comprehensive crotonylation proteome indicated diverse functions for lysine crotonylation in papaya.

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“…Measurements of the physiological parameters of papaya fruit were performed according to the previous study [15]. Three biological replicate were used.…”

Section: Methodsmentioning

confidence: 99%

“…Papaya ( Carica papaya L.), a climacteric fruit widely planted and consumed, displays quick softening and a short shelf-life [15, 16]. Once the climacteric fruit is mature, its soft texture and susceptibility to pathogenic fungi make the storage life short [17].…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analysis provides novel insights into the regulation mechanism underlying papaya (Carica papaya L.) exocarp during fruit ripening process

Bian

et al. 2019

BMC Plant Biol

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Background Papaya ( Carica papaya L.) is a popular climacteric fruit, undergoing various physico-chemical changes during ripening. Although papaya is widely cultivated and consumed, few studies on the changes in metabolism during its ripening process at the proteasome level have been performed. Using a newly developed TMT-LCMS analysis, proteomes of papaya fruit at different ripening stages were investigated. Results In total, 3220 proteins were identified, of which 2818 proteins were quantified. The differential accumulated proteins (DAPs) exhibited various biological functions and diverse subcellular localizations. The KEGG enrichment analysis showed that various metabolic pathways were significantly altered, particularly in flavonoid and fatty acid metabolisms. The up-regulation of several flavonoid biosynthesis-related proteins may provide more raw materials for pigment biosynthesis, accelerating the color variation of papaya fruit. Variations in the fatty acid metabolism- and cell wall degradation-related proteins were investigated during the ripening process. Furthermore, the contents of several important fatty acids were determined, and increased unsaturated fatty acids may be associated with papaya fruit volatile formation. Conclusions Our data may give an intrinsic explanation of the variations in metabolism during the ripening process of papaya fruit. Electronic supplementary material The online version of this article (10.1186/s12870-019-1845-4) contains supplementary material, which is available to authorized users.

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Identification, Expression and IAA-Amide Synthetase Activity Analysis of Gretchen Hagen 3 in Papaya Fruit (Carica papaya L.) during Postharvest Process

Cited by 17 publications

References 57 publications

The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors

The Gene Structure and Expression Level Changes of the GH3 Gene Family in Brassica napus Relative to Its Diploid Ancestors

A qualitative proteome-wide lysine crotonylation profiling of papaya (Carica papaya L.)

Comparative proteomic analysis provides novel insights into the regulation mechanism underlying papaya (Carica papaya L.) exocarp during fruit ripening process

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