Identification, evolutionary and expression analysis of the galactinol synthase (GolS) genes in Panicum virgatum L. and Panicum hallii : An in silico approach

Gois, Eduardo Henrique Baltrusch de; Menegazzo, Renato Fernando; Santos, Tiago Benedito dos; Souza, Silvia Graciele Hülse de

doi:10.1016/j.plgene.2020.100262

Cited by 2 publications

(1 citation statement)

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“…GolS gene has been identified and characterized in different plant species, both in monocot and dicot: Arabidopsis thaliana (Nishizawa et al, 2008), Coffea arabica L. and C. canephora (Santos et al, 2011;, Pisum sativum (Lahuta et al, 2014), Solanum lycopersicum and Brachypodium distachyon (Filiz et al, 2015), Manihot esculenta Crantz (Li et al, 2018), Musa acuminata (Dolcimasculo et al, 2018), Panicum virgatum L. and P. hallii (Góis et al, 2020), Phaseolus vulgaris L. (Koning et al, 2023). Plants have more than one isoform of GolS and can act on specific tissues (Sengupta et al, 2012;revised by Santos;Vieira, 2020).…”

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confidence: 99%

Characterization of Galactinol Synthase (Gols) Genes in Ipomoea Triloba and I. Trifida: An Approach Using Bioinformatics

Rufino,

Lobo,

Yaguinuma

et al. 2024

COLLOQ AGRARIAE

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Galactinol synthase (GolS - EC 2.4.1.123) is classified as a key enzyme that catalyzes the first step in the synthesis pathway of the raffinose family (RFOs). Although GolS genes have been characterized in several important species, their characterization in sweet potatoes has yet to be explored. Ipomoea trifida (Kunth) G. Don (2n = 2x = 30) is currently described as one of the closest ancestors of the sweet potato and considered an excellent crossbreeding species, allowing the introgression of important genes such as GolS. This study aimed to identify and characterize in silico the GolS genes in I. trifida and compare with I. triloba. We identified nine GolS genes, five in I. triloba and four in I. trifida. Our study encompassed various aspects, including gene structure analysis, motif identification, chromosomal distribution, synteny analysis, and gene expression. The presence of gene duplications and purifying selection were highlighted, suggesting the evolutionary significance of GolS genes in these species. Phylogenetic analysis categorized GolS proteins into three groups, potentially reflecting distinct functional roles. Furthermore, synteny analysis revealed orthologous relationships between GolS genes in the studied species and related plants, contributing to our understanding of their evolutionary history. In silico expression analysis across diverse tissues unveiled tissue-specific expression patterns, hinting at specialized roles for GolS genes in different plant organs. These findings contribute to the broader field of plant genetics, carbohydrate metabolism, and agriculture, offering opportunities for crop improvement and sustainable food production.

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