Identification, cloning and tissue localization of a rainbow trout (Oncorhynchus mykiss) intelectin-like protein that binds bacteria and chitin

Russell, Samantha; Young, Karrie; Smith, Marta S.; Hayes, Michael; Lumsden, John S.

doi:10.1016/j.fsi.2008.02.018

Cited by 39 publications

(21 citation statements)

References 50 publications

(74 reference statements)

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“…These intelectins include the grass carp intelectin, the rainbow trout intelectin, zINTL1, zINTL2, hINTL1, hINTL2, mINTL1, and mINTL2. Among which, zINTL1, hINTL1, the grass carp intelectin, and the rainbow trout intelectin are also highly expressed in several other tissues besides the gut [3,6,25,31]. However, as descried previously, their expressions in other tissues are highly different although they are all highly expressed in gut.…”

Section: Intelectin Family Members Also Vary Considerablymentioning

confidence: 66%

“…Another liver predominant intelectin described to date is the channel catfish (Ictalurus punctatus) intelectin 2 [23]. Besides, some other fish intelectins were also found to be expressed in liver [23][24][25], although not in a predominant level. Different from fish intelectins, all human, mouse, and frog intelectins reported to date were not expressed in liver.…”

Section: Tissue Distribution Of Zintls In Adult Zebrafish With or Witmentioning

confidence: 98%

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Characterization and comparative analyses of zebrafish intelectins: Highly conserved sequences, diversified structures and functions

Lin¹,

Cao²,

Su³

et al. 2009

Fish & Shellfish Immunology

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Section: Intelectin Family Members Also Vary Considerablymentioning

confidence: 66%

Section: Tissue Distribution Of Zintls In Adult Zebrafish With or Witmentioning

confidence: 98%

Characterization and comparative analyses of zebrafish intelectins: Highly conserved sequences, diversified structures and functions

Lin¹,

Cao²,

Su³

et al. 2009

Fish & Shellfish Immunology

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“…VHSV can also be purified using the plant lectin concanavalin-A, which primarily recognizes mannose groups (Perez et al 1998) but also binds agarose (Bernhard & Avrameas 1971), a monosaccharide derivative of glucose. Rainbow trout intelectin, by contrast, is known to bind mannose and N-acetyl-glucosamine (Russell et al 2008b). Previous work with numerous animal and plant lectins has shown that galactose-and galactosamine-specific lectins cannot aggregate several isolates of VHSV genotype 1 while another VHSV isolate was poorly bound by concavalin-A (Bernard et al 1985) suggesting that surface carbohydrate is highly strain specific and may influence the ability of the innate immune system to recognise different isolates.…”

Section: Discussionmentioning

confidence: 99%

“…Blots were developed with ECL-Plus Western Blotting Detection kit (GE Healthcare Biosciences) and were visualized on the Typhoon 9410 Imaging System (GE Healthcare Biosciences). Rainbow trout intelectin was identified in an identical fashion except for the use of polyclonal rabbit anti-rainbow trout intelectin antibody (Russell et al 2008b(Russell et al , 2009 Russell et al (2008a). Whole rainbow trout plasma was used as a control for column fractions.…”

mentioning

confidence: 99%

Rainbow trout Oncorhynchus mykiss ladderlectin, but not intelectin, binds viral hemorrhagic septicemia virus IVb

Reid¹,

Young²,

Lumsden³

2011

Dis. Aquat. Org.

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The innate immune system of fish is critical for rapid detection and immediate response to infection, as well as to orchestrate the adaptive branch of the immune system. Rainbow trout Oncorhynchus mykiss ladderlectin and intelectin are plasma pattern recognition receptors (PRR) for bacterial and fungal pathogens of rainbow trout, but their role as PRRs for virus is unknown. Viral hemorrhagic septicemia virus (VHSV) IVb is a recently described fish pathogen in the Great Lakes, and rainbow trout can be experimentally infected. Using an indirect enzyme-linked immunosorbent assay, rainbow trout plasma ladderlectin significantly (p < 0.05) bound purified VHSV while int electin did not. In addition, plasma ladderlectin but not intelectin was eluted from a VHSV-conjugated Toyopearl column using EDTA. Protein identification was confirmed with polyclonal antiserum used with slot immunoblot, 1-dimensional sodium dodecyl sulphate polyacrylamide electrophoresis, and Western immunoblot.KEY WORDS: Oncorhychus mykiss · Pattern recognition receptor · Viral hemorrhagic septicemia virus · Innate immunity · Plasma proteins · Binding · Ladderlectin · Intelectin Resale or republication not permitted without written consent of the publisherDis Aquat Org 95: [137][138][139][140][141][142][143] 2011 in rainbow trout by 7 d and lasting up to 21 d (Acosta et al. 2005). A DNA vaccine for VHSV conferred shortterm protection of rainbow trout Oncorhynchus mykiss from infection with heterologous rhabdoviruses and the unenveloped nodavirus of turbot (Sommerset et al. 2003). Resistance was hypothesized to be due to the initial inflammatory response that is likely to produce innate antiviral proteins such as interferon and Mx (Sommerset et al. 2003). Mx proteins are one of the major families of interferon-stimulated proteins resulting from innate immune recognition of viral infection (Ellis 2001). More recently, VHSV has been shown to stimulate multiple components of the innate immune system. In gilthead seabream Sparus aurata, VHSV has been shown to recruit and increase the activity of natural cytotoxic cells (NCC) (Esteban et al. 2008). Rainbow trout TLR9 was upregulated at the site of injection of VHSV glycoprotein G in muscle, suggesting the recruitment of dendritic cells to the site; in the head kidney and spleen there was transcriptional increase of tumor necrosis factor alpha and interleukin-6, which are cytokines known to be upregulated by TLR9 activated by unmethylated CpG DNA (Ortega-Villaizan et al. 2009). In addition, the RIG-I/MAVS pathway of double stranded RNA-stimulated interferon induction has been documented in numerous fish species (Biacchesi et al. 2009), and Mx transcript upregulation has been shown in rainbow trout (Acosta et al. 2005) in response to VHSV infection. In Japanese flounder Paralichthys olivaceus, VHSV infection upregulates the hepatic expression of C3 and complement regulatory proteins (Byon et al. 2006).Lectins are cell-associated or circulating proteins involved in recognition and binding of vir...

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“…The purported role of intelectins is innate stimulation and opsonisation for phagocytic enhancement (Tsuji et al, 2001, Russell et al, 2008. It has been proposed from zebrafish (D. rerio) genomic analysis that intelectins may evolutionarily belong to four separate clades in mammal, frog, fish, and lancet.…”

Section: Pathogen Activation Of Nets Has Identified a Range Of Stimulmentioning

confidence: 99%

Immune defence mechanisms of barramundi (Lates calcarifer) peripheral blood against Streptococci

Masterman¹

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IMMUNE DEFENCE MECHANISMS OF BARRAMUNDI (Lates calcarifer) PERIPHERAL BLOOD AGAINST STREPTOCOCCIAquaculture now supplies more than half of the fish used for human consumption and is a major contributor to global food security. As aquaculture growth must be sustained to continue to supply increasing population-driven demand for food fish, efficiency must be increased. In spite of major inroads in disease control through mass vaccination of farm fish, bacterial diseases continue to cause substantial losses, particularly in tropical and warmtemperate regions where aquaculture is expanding fastest. To develop new vaccines, and improve existing vaccines for effective disease control in these regions, an improved understanding of the mechanisms of pathogen immune evasion and dissemination within the host may identify new vaccine targets. Streptococcus agalactiae and Streptococcus iniae are significant pathogens of warm and temperate farmed and wild fish. Both have a broad host range, express a polysaccharide capsule as a major virulence determinant and cause similar pathologies characterized by rapid sepsis, followed by meningitis and death. S. iniae causes significant mortality in barramundi, Lates calcarifer. Interestingly, S. agalactiae ST261, although causing mortality in wild fish along the Queensland coast, does not appear to cause mortality in barramundi farmed in close proximity. This difference provides a basis for comparison, in order to explore the requirements for blood colonization and sepsis by S. iniae in barramundi, and to increase our understanding of sepsis in fish.S. iniae grew rapidly in barramundi blood, doubling in less than 30 minutes in a whole blood bactericidal assay. In contrast, S. agalactiae was unable to multiply. Moreover, E. coli DH5 was completely killed in barramundi blood during the same incubation period, suggesting 3 that antibacterial humoral and cellular immune defences were functional within the blood bactericidal model. A capsular defective strain of S. iniae also survived in the bloodbactericidal assay, however the rapid proliferation was reduced, suggesting that while the capsule is important for bacterial colonisation it is not the only means used to evade bloodborne defences. As gram-positive pathogens are particularly susceptible to lysozyme due to the dominance of muramic acids in the cell wall, the role of lysozyme was investigated.Lysozyme levels in barramundi serum and plasma were undetectable in a lysis assay using Micrococcus lysodeitikus, indicating very low levels of circulating lysozyme in healthy barramundi.The first responder immune cells in blood are neutrophils and these are critical in the prevention of sepsis; for example, granulocytopenia (circulating granulocyte deficiency) is associated with increased susceptibility to Escherichia coli K1 and Klebsiella pneumoniae sepsis in neonates, and this can be partially repaired by injection of granulocyte colony stimulating factor (G-CSF) to increase neutrophil numbers (Deshmukh et al., 2014). This critic...

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Identification, cloning and tissue localization of a rainbow trout (Oncorhynchus mykiss) intelectin-like protein that binds bacteria and chitin

Cited by 39 publications

References 50 publications

Characterization and comparative analyses of zebrafish intelectins: Highly conserved sequences, diversified structures and functions

Characterization and comparative analyses of zebrafish intelectins: Highly conserved sequences, diversified structures and functions

Rainbow trout Oncorhynchus mykiss ladderlectin, but not intelectin, binds viral hemorrhagic septicemia virus IVb

Immune defence mechanisms of barramundi (Lates calcarifer) peripheral blood against Streptococci

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