Identification, cloning, and characterization of a major cat flea salivary allergen (Cte f 1)

McDermott, Martin J.; Weber, Eric; Hunter, S W; Stedman, Kim E.; Best, E A; Frank, Glenn; Wang, Ruth; Escudero, Jean; Kuner, Jerry; McCall, Catherine

doi:10.1016/s0161-5890(00)00061-4

Cited by 49 publications

(31 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This prokaryotic expression system is still applied for the production of many different recombinant allergens [103, 104, 105, 106, 107, 108]. In more recent years, several allergens were produced in eukaryotic expression systems like the yeast Pichia pastoris [109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127], insect cells [128, 129, 130, 131, 132, 133, 134, 135, 136], and tobacco plants [137, 138]. These systems facilitate post-translational modifications that do not (easily) occur in E. coli , like disulfide-bridge formation, hydroxylation of prolines, glycosylation.…”

Section: Recombinant Allergens For Application In Diagnostics and Immmentioning

confidence: 99%

“…If correct folding is not achieved, eukaryotic expression systems usually offer the solution. As mentioned above, several allergens have now been produced in the yeast P. pastoris [109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127]. In general, yeast is known for hyperglycosylation with large high-mannose glycans [90, 94].…”

Section: Recombinant Allergens For Application In Diagnostics and Immmentioning

confidence: 99%

See 1 more Smart Citation

Carbohydrate Epitopes and Their Relevance for the Diagnosis and Treatment of Allergic Diseases

Ree¹

2002

Int Arch Allergy Immunol

173

119

View full text Add to dashboard Cite

Allergenicity of plant and invertebrate N-glycans has been shown to be caused by the presence of two typical nonmammalian substitutions: an α(1,3)-fucose linked to the proximal N-acetylglucosamine and a β(1,2)-xylose linked to the core mannose. IgE antibodies against these carbohydrate structures are induced upon exposure to pollen or after insect stings, and result in extensive cross-reactivity to plant and invertebrate foods. These cross-reactive IgE antibodies have been shown to possess variable degrees of biological activity, but have never been convincingly shown to induce clinical food allergy. The most likely explanation for this lack of clinical relevance has to be sought in a combination of epitope valency and antibody affinity. In diagnostic tests, these antibodies are at the basis of many false-positive test results for food allergy. Recombinant technologies offer the possibility to produce allergens that do not carry IgE-binding glycans. Whether their absence or presence is of importance for the application of recombinant allergens in immunotherapy is still largely unknown.

show abstract

Section: Recombinant Allergens For Application In Diagnostics and Immmentioning

confidence: 99%

Section: Recombinant Allergens For Application In Diagnostics and Immmentioning

confidence: 99%

Carbohydrate Epitopes and Their Relevance for the Diagnosis and Treatment of Allergic Diseases

Ree¹

2002

Int Arch Allergy Immunol

173

119

View full text Add to dashboard Cite

show abstract

“…Beside E. coli, the yeasts Saccharomyces cerevisiae [43, 90, 137]and Pichia pasteuris [84, 130, 138, 139, 140]have been used to efficiently express allergens not easily produced by E. coli . In two cases, rLep d 2 [95]and hyaluronidase from bee venom [44], the allergenic proteins have been produced using insect cells infected with baculovirus.…”

Section: Sources Of Rallergens Used In Skin Testsmentioning

confidence: 99%

Recombinant Allergens for Skin Testing

Schmid‐Grendelmeier¹,

Crameri²

2001

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Skin testing is a basic diagnostic procedure widely used to explore immediate-type reactions to allergen preparations in vivo. Despite their reliability, if standardized extracts are used, skin tests suffer from limited reproducibility due to difficulties in preparing consistently standardized extracts from natural raw material. Starting from allergen-encoding cDNAs, large amounts of highly pure allergens with a high batch-to-batch consistency satisfying the quality requirements of medicinal products manufactured by recombinant DNA technology can be produced. These reagents are expected to be qualitatively superior to the commercially available allergen preparations used for the in vitro and in vivo diagnosis of allergic conditions. In this article the current literature available on skin testing with such recombinant allergens (rAllergens) is reviewed and critically analyzed. To date many different rAllergens of various pollens, moulds, mites, bee venom, latex and celery have been used in skin testing in more than 1,600 allergic and control individuals. Skin prick tests as well as intradermal skin tests with rAllergens prove to be highly specific and safe. The diagnostic sensitivity of single rAllergens is generally lower than those obtained with allergen extracts, but can be considerably increased by using rAllergen panels covering the most important allergenic structures present in a given complex allergenic extract. Moreover, quantitative skin testing with single rAllergens allows interesting insights into correlations between the in vivo and in vitro sensitization to a given allergen. In conclusion, skin testing with rAllergens offers a highly specific and safe additional diagnostic tool to elucidate patient- and disease-specific sensitization patterns which will be needed for the development of patient-tailored immunotherapeutic treatments.

show abstract

“…Tunga penetrans causa una ectoparasitosis bastante frecuente en algunos países de Latinoamérica y el Caribe, que se propaga por la postura de huevos de la hembra en la piel de huéspedes infectados (34,35 Desde el punto de vista de la alergología, la pulga más estudiada ha sido C. felis. En estudios en perros se han identificado tres alérgenos de pulga (39). También, hay estudios en humanos en los cuales se ha demostrado que los pacientes con urticaria papular generan IgE con varios componentes del extracto de pulga (26,40).…”

Section: Agentes Etiológicosunclassified

Papular urticaria: A review of causal agents in Colombia

et al. 2016

View full text Add to dashboard Cite

López: análisis bioinformático y datos experimentales inéditos Todos los autores contribuyeron en igual medida a la redacción del manuscrito. La urticaria papular es una enfermedad alérgica causada por la picadura de insectos, la cual predomina en el trópico. El objetivo de esta revisión fue profundizar en sus aspectos epidemiológicos e inmunológicos, particularmente con base en datos publicados en Latinoamérica. Se hizo una revisión no sistemática mediante la búsqueda electrónica de artículos sobre la epidemiología de la urticaria papular, las características entomológicas de los agentes causales y los mecanismos inmunológicos asociados. Según los diversos reportes de centros médicos de Latinoamérica la urticaria papular es frecuente; el único estudio de prevalencia publicado indica que afecta a una cuarta parte de los niños escolares de Bogotá. Hay información sobre la relación causal entre la exposición domiciliaria a la pulga, la pobreza y la urticaria papular en Bogotá, una ciudad representativa de las altitudes andinas. No hay estudios que indaguen directamente sobre los insectos causales en zonas cálidas, aunque se sospecha clínicamente de los mosquitos Aedes aegypti y Culex quinquefasciatus. En cuanto a su patogenia, se destaca la participación de mecanismos celulares que involucran las células colaboradoras Th2, lo cual explica que sea una condición de hipersensibilidad retardada. El papel de la inmunoglobulina E (IgE) en la urticaria papular no está tan claro. Se desconocen los antígenos derivados de los insectos que causan la enfermedad, aunque se plantea que existen moléculas comunes de reacción cruzada entre los insectos, tales como el alérgeno Cte f 2 en la pulga, y sus homólogos en los mosquitos. La urticaria papular es una condición frecuente en Latinoamérica que debe investigarse en profundidad. La caracterización inmunológica de los componentes moleculares que causan esta condición puede resolver interrogantes sobre su etiología y su patogenia. Urticaria papular y sus agentes causales en ColombiaPalabras clave: urticaria; insectos; Aedes; Culex; Ctenocephalides; hipersensibilidad. doi: http://dx.doi.org/10.7705/biomedica.v36i4.3258 Papular urticaria: A review of causal agents in ColombiaPapular urticaria is a chronic allergic reaction induced by insect bites, which is common in the tropics. The objective of this review was to deepen on epidemiological and immunological aspects of this disease, focused on data published in Latin American countries. We conducted a non-systematic review of the literature through electronic search on the epidemiology of papular urticaria, the entomological characteristics of the causative agents and associated immunological mechanisms. Several reports from medical centers suggest that papular urticaria is common in Latin America. Only one epidemiological survey designed to estimate prevalence of papular urticaria has been published, reporting that about a quarter of children under six years of age is affected by this condition in Bogotá. There is evidence on the ca...

show abstract

Identification, cloning, and characterization of a major cat flea salivary allergen (Cte f 1)

Cited by 49 publications

References 26 publications

Carbohydrate Epitopes and Their Relevance for the Diagnosis and Treatment of Allergic Diseases

Carbohydrate Epitopes and Their Relevance for the Diagnosis and Treatment of Allergic Diseases

Recombinant Allergens for Skin Testing

Papular urticaria: A review of causal agents in Colombia

Contact Info

Product

Resources

About