Identification, Characterization and Phylogenic Analysis of Conserved Genes within the p74 Gene Region of Choristoneura fumiferana Granulovirus Genome

Rashidan, Kianoush Khajeh; Nassoury, Nasha; Giannopoulos, Paresa N.; Mauffette, Yves; Guertin, Claude

doi:10.5483/bmbrep.2004.37.6.700

Cited by 1 publication

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References 63 publications

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“…In GVs, PiraGV (Pierididae) was most closely related with tortricid-infecting GVs such as CpGV, CrleGV, AdorGV as well as gelechiid-infecting PhopGV, these constituted a clade (with a high bootstrapping support NJ: 100%) that is clearly different from the noctuidinfecting GVs. This seemed to agree with others' research that GV genera could be further divided into two groups [30][31][32][33][34]. Similar phylogenetic analysis results were obtained with trees generated by most parsimony (MP) and minimum evolution (ME) programs (data not shown).…”

Section: Phylogenetic Analysissupporting

confidence: 90%

Identification, transcriptional and phylogenetic analysis of the DNA polymerase gene of Pieris rapae granulovirus

Wen

Chen

2007

Virus Genes

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The DNA polymerase gene (dnapol) of the Pieris rapae granulovirus (PiraGV) was completely sequenced and located between 73.1 and 76 m.u. on the PiraGV genome. Its open reading frame (ORF) has 3135 nucleotides (35% G-C content) encoding 1045 amino acids with a predicted molecular mass of 122.16 kDa. Homology analysis indicated that PiraGV dnapol had 28-66% amino acid identity to that of other known baculoviruses. Comparative sequence analyses demonstrated that the PiraGV dnapol gene contains conserved 3'-5' exonuclease motifs and DNA binding functional domains of the DNA polymerase enzyme found in all known baculovirus dnapols. Northern blot results showed that in infected Pieris rapae larvae the PiraGV dnapol gene was transcribed as a predominant 3.7 kb mRNA. 5' and 3' RACE indicated that the PiraGV dnapol transcript was initiated from the thymine residue located at -378 nt upstream from the ATG start codon and terminated at the polyadenylation signal AATAAA. Phylogenetic analysis of dnapol sequences suggests that the PiraGV dnapol is more closely related to that of Cydia pomonella GV and Cryptophlebia leucotreta GV than to those of other baculoviruses.

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