Identification, characterization and immunological analysis of Ras related C3 botulinum toxin substrate 1 (Rac1) from grass carp Ctenopharyngodon idella

Hu, Moyan; Shen, Yubang; Xu, Xiaoyan; Yu, Hongyan; Zhang, Meng; Dang, Yunfei; Li, Jiale

doi:10.1016/j.dci.2015.08.010

Cited by 29 publications

(9 citation statements)

References 35 publications

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“…The up-regulated expression pattern was consistent with the observations in studies of other aquaculture species. In grass carp, Rac1 was shown to be inducible by Aeromonas hydrophila in vivo and in vitro (Hu et al, 2016); in turbot, the expression level of Rac1 increased significantly at 8 h and 24 h in liver after challenge with Vibrio harveyi, suggesting an immunologic function of this Rho family member (Jia and Zhang, 2009). In this study, Rac2 was found to be differentially expressed at 24 h after F. columnare infection.…”

Section: Expression Analysis Of Rho Gtpase Genes After Bacterial Infesupporting

confidence: 46%

Identification, annotation and expression analysis of 29 Rho GTPase genes from channel catfish (Ictalurus punctatus) after bacterial infections

Tan¹,

Yao²,

Zhou³

et al. 2017

Developmental & Comparative Immunology

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The Rho family GTPases are a group of small monomeric G proteins, which are molecular switches in signaling pathways. They have been known to regulate a diverse range of cellular processes including actin cytoskeleton rearrangement and microtubule dynamics. In particular, their participations in immune responses are also significant. However, little information of the Rho GTPases is available in teleost including channel catfish, an economically important species and one of the best teleost models forimmunological research. In this study, Rho GTPase genes were identified from channel catfish and well annotated by phylogenetic and syntenic analyses. Their expression profiles were determined in channel catfish healthy tissues and infected tissues. Altogether seven Rho GTPase genes were significantly regulated after bacterial infection, with six genes in the gill after Flavobacterium columnare challenge and two genes in the intestine in response to Edwardsiella ictaluri. All the differentially expressed genes were up-regulated soon after bacterial infection. Different expression patterns between the two experiments were observed, which may be attributed to tissue-specific regulation or pathogen-specific regulation. These results suggested that Rho GTPases play important roles in immune responses to bacterial pathogens, setting a foundation for future investigation on Rho GTPases.

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Section: Expression Analysis Of Rho Gtpase Genes After Bacterial Infesupporting

confidence: 46%

Identification, annotation and expression analysis of 29 Rho GTPase genes from channel catfish (Ictalurus punctatus) after bacterial infections

Tan¹,

Yao²,

Zhou³

et al. 2017

Developmental & Comparative Immunology

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“…All experiments were performed in triplicate, providing technical repeats. Expression levels of the different genes were analyzed using the comparative CT method (2 −ΔΔCT ) [ 50 ].…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

Dang

Shen

et al. 2016

PLoS ONE

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The grass carp (Ctenopharyngodon idella) is an important commercial farmed herbivorous fish species in China, but is susceptible to Aeromonas hydrophila infections. In the present study, we performed de novo RNA-Seq sequencing of spleen tissue from specimens of a disease-resistant family, which were given intra-peritoneal injections containing PBS with or without a dose of A. hydrophila. The fish were sampled from the control group at 0 h, and from the experimental group at 4, 8, 12, 24, 48 and 72 h. 122.18 million clean reads were obtained from the normalized cDNA libraries; these were assembled into 425,260 contigs and then 191,795 transcripts. Of those, 52,668 transcripts were annotated with the NCBI Nr database, and 41,347 of the annotated transcripts were assigned into 90 functional groups. 20,569 unigenes were classified into six main categories, including 38 secondary KEGG pathways. 2,992 unigenes were used in the analysis of differentially expressed genes (DEGs). 89 of the putative DEGs were related to the immune system and 41 of them were involved in the complement and coagulation cascades pathway. This study provides insights into the complement and complement-related pathways involved in innate immunity, through expression profile analysis of the genomic resources in C. idella. We conclude that complement and complement-related genes play important roles during defense against A. hydrophila infection. The immune response is activated at 4 h after the bacterial injections, indicating that the complement pathways are activated at the early stage of bacterial infection. The study has improved our understanding of the immune response mechanisms in C. idella to bacterial pathogens.

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“…The primers used are shown in Table . Expression levels of sEH genes were analyzed using the comparative ΔΔCt method . The relative mRNA expression levels of the sEH gene in S. maltophilia DM‐2 was compared with that of the gyrB gene.…”

Section: Methodsmentioning

confidence: 99%

Monensin biodegradation pathway and role of epoxide hydrolase in Stenotrophomonas maltophilia DM‐2

Wan

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND: Monensin is widely used in livestock and poultry production to promote animal growth and control coccidiosis. Most monensin is excreted as unchanged parent compounds via feces. However，monensin is very stable and difficult to degrade in the environment, and is potentially risky for the health of wildlife and humans. To date, the biodegradation process of monensin by bacteria and its mechanism is unclear. In this study, we found that a Stenotrophomonas maltophilia DM-2 strain isolated from chicken manure could effectively degrade monensin.RESULT: The optimum temperature and pH for DM-2 to degrade monensin were 40°C and pH 7.0. A potential degradation pathway of monensin was proposed based on the identification and characterization of two new monensin metabolic intermediates by liquid chromatography quadrupole time-of-flight mass spectroscopy. In addition, an inducible monensin-degrading activity was present in DM-2. A soluble epoxide hydrolysis (sEH) gene highly expressed in the DM-2 on day 3 and 6 under monensin stress, which was not observed in the DM-2 without monensin stress. When a specific inhibitor TPPU (1-(1-propanoylpiperidin-4-yl)-3-[4-(trifluoromethoxy)phenyl]urea) of sEH was added into the cell-free extract, the degradation of monensin was inhibited.CONCLUSION: Strain DM-2 can biodegrade monensin by soluble epoxy hydrolase and produce two new intermediates. To our knowledge, this is the first report of the degradation of monensin by Stenotrophomonas strains. The novel metabolic pathways of monensin which we found may provide new insight into the environmental fate of monensin.

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Identification, characterization and immunological analysis of Ras related C3 botulinum toxin substrate 1 (Rac1) from grass carp Ctenopharyngodon idella

Cited by 29 publications

References 35 publications

Identification, annotation and expression analysis of 29 Rho GTPase genes from channel catfish (Ictalurus punctatus) after bacterial infections

Identification, annotation and expression analysis of 29 Rho GTPase genes from channel catfish (Ictalurus punctatus) after bacterial infections

Transcriptome Analysis of the Innate Immunity-Related Complement System in Spleen Tissue of Ctenopharyngodon idella Infected with Aeromonas hydrophila

Monensin biodegradation pathway and role of epoxide hydrolase in Stenotrophomonas maltophilia DM‐2

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