Identification, Characterization and Genetic Improvement of Bacteriocin Producing Lactic Acid Bacteria

Abdelhadi, Abdelhadi A.; Elarabi, Nagwa I.; Salim, Rasha G.; Sharaf, Ahmed; Abosereh, Nivien A.

doi:10.3923/biotech.2016.76.85

Cited by 9 publications

(4 citation statements)

References 20 publications

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“…These problems are incentives to search for alternative safe and effective control measures with different modes of action. Biological control agents can be used where chemical pesticides are banned or where pests have developed resistance to standard chemicals and play an important role in integrated pest management (IPM) programs (Abdelhadi et al 2016 andvan Lenteren et al 2018). Among these approaches is the use of the entomopathogenic bacterium, Bacillus thuringiensis (Bt) that provides a valuable alternative to chemical insecticides.…”

Section: Introductionmentioning

confidence: 99%

Alterations in the expression of certain midgut genes of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and midgut histopathology in response to Bacillus thuringiensis Cry1C toxin

Khalil

Moussa

Zein

et al. 2021

Egypt J Biol Pest Control

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Background Bacillus thuringiensis (Bt) utilization as a biological control agent is highly recommended due to its safety, specificity, and efficiency. Importance of the entomocidal Cry proteins secreted by Bt is dramatically increased subsequent Cry genes transformation into a number of economic crops, rendering them protection against insect attack. In the last decade, insect resistance against transgenic Bt crops is gradually raised in several lepidopteran pests. A better understanding of the processing of Bt Cry1C toxin in the larval midgut of the lepidopteran pest species, the cotton leaf worm, Spodoptera littoralis (Boisd.), is very important to characterize the main regulatory elements of Bt tolerance. Results The present study aimed to define factors that are involved in insect tolerance toward Bt Cry1C through evaluating the mRNA level of trypsin (Try), aminopeptidase N (APN), alkaline phosphatase (ALP), cadherin (Cad), and cytochrome P450 (CYP) in both susceptible and cry1C tolerant strains of S. littoralis. Total RNAs were extracted from susceptible and tolerant strains to construct cDNAs. Quantitative real-time polymerase chain reaction (qPCR) showed a significant upregulation of CYP gene in tolerant strain. In contrast, the levels of expression of Try, ALP, and Cad were significantly downregulated in tolerant strain. APN relative mRNA expression did not show significant differences between susceptible and tolerant strains. Histologically, the midgut of late third-instar larvae of tolerant population S. littoralis showed vacuolization of the epithelium and disruption of both the peritrophic membrane and the striated boarder compared to the susceptible strain. Conclusions Obtained data indicated a relationship between exposing to Bt Cry1C toxin and alteration of CYP, Try, ALP, and Cad expression in midgut of S. littoralis. These results may be an evidence for the important roles of CYP, Try, ALP, and Cad in the resistance development and toxicity to Bt Cry1C. The results are useful for further illustrating of Bt Cry1C processing and S. littoralis tolerance.

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Section: Introductionmentioning

confidence: 99%

Alterations in the expression of certain midgut genes of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and midgut histopathology in response to Bacillus thuringiensis Cry1C toxin

Khalil

Moussa

Zein

et al. 2021

Egypt J Biol Pest Control

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“…Also, it was predicted the presence of genes encoded for resistance to antibiotics like fluoroquinolones, fosfomycin, β-lactamase the mdtABCDKLNO multidrug resistance cluster, and multidrug resistance efflux pumps. Potential antimicrobial compounds could be produced by microbes during bioremediation process (Abdelhadi et al 2016 ; El-Arabi et al 2018 ). From the previous results, it is revealed that R. planticola FACU 3 multi heavy metals resistance bacteria other than lead.…”

Section: Discussionmentioning

confidence: 99%

“…SN023-0100, Taiwan) utilizing the manufacturer's instructions. Two universal primers [27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′- GGTTACCTTGTTACGACTT-3′)] for 16S rRNA gene were used (Abdelhadi et al 2016 ). PCR reaction proceeded in 50 μL total volume, containing 50 ng/μL DNA (5 μl), 2X (25 μL) One PCR™ master mix (Gene Direx, cat.…”

Section: Methodsmentioning

confidence: 99%

Draft genome of Raoultella planticola, a high lead resistance bacterium from industrial wastewater

et al. 2023

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Isolation of heavy metals-resistant bacteria from their original habitat is a crucial step in bioremediation. Six lead (Pb) resistant bacterial strains were isolated and identified utilizing 16S rRNA to be Enterobacter ludwigii FACU 4, Shigella flexneri FACU, Microbacterium paraoxydans FACU, Klebsiella pneumoniae subsp. pneumonia FACU, Raoultella planticola FACU 3 and Staphylococcus xylosus FACU. It was determined that all these strains had their Minimum inhibitory concentration (MIC) to be 2500 ppm except R. planticola FACU 3 has a higher maximum tolerance concentration (MTC) up to 2700 ppm. We evaluated the survival of all six strains on lead stress, the efficiency of biosorption and lead uptake. It was found that R. planticola FACU 3 is the highest MTC and S. xylosus FACU was the lowest MTC in this evaluation. Therefore, transmission electron microscopy (TEM) confirmed the difference between the morphological responses of these two strains to lead stress. These findings led to explore more about the genome of R. planticola FACU 3 using illumine Miseq technology. Draft genome sequence analysis revealed the genome size of 5,648,460 bp and G + C content 55.8% and identified 5526 CDS, 75 tRNA and 4 rRNA. Sequencing technology facilitated the identification of about 47 genes related to resistance to many heavy metals including lead, arsenic, zinc, mercury, nickel, silver and chromium of R. planticola FACU 3 strain. Moreover, genome sequencing identified plant growth-promoting genes (PGPGs) including indole acetic acid (IAA) production, phosphate solubilization, phenazine production, trehalose metabolism and 4-hydroxybenzoate production genes and a lot of antibiotic-resistant genes.

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“…16S rRNA amplification by polymerase chain reaction (PCR) was utilized. Two universal primers (27F and 1492R) were utilized for 16S rRNA fragment 1520 bp amplification (Additional file 1 : Table S1) (Abdelhadi et al 2016 ). One percentage agarose gel was used for PCR reaction visualization under UV light.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of isoproturon by Escherichia coli expressing a Pseudomonas putida catechol 1,2-dioxygenase gene

Elarabi,

Abdelhadi,

Nassrallah

et al. 2023

AMB Expr

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The phenylurea herbicides are persistent in soil and water, necessitating the creation of methods for removing them from the environment. This study aimed to examine the soil microbial diversity, searching for local bacterial isolates able to efficiently degrade the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1, 1-dimethylurea (IPU). The best isolates able to effectively degrade IPU were selected, characterized, and identified as Pseudomonas putida and Acinetobacter johnsonii. The catechol 1, 2-dioxygenase enzyme's catA gene was amplified, cloned, and expressed in E. coli M15. The Expressed E. coli showed high degradation efficiency (44.80%) as analyzed by HPLC after 15 days of inoculation in comparison to P. putida (21.60%). The expression of the catA gene in P. putida and expressed E. coli was measured using quantitative polymerase chain reaction (qPCR). The results displayed a significant increase in the mRNA levels of the catA gene by increasing the incubation time with IPU. Hydrophilic interaction chromatography (HILIC) mass spectrometry analysis revealed that three intermediate metabolites, 1-(4-isopropylphenyl)-3-methylurea (MDIPU), 4-Isopropylaniline (4-IA) and 1-(4-isopropylphenyl) urea (DDIPU) were generated by both P. putida and expressed E. coli. In addition, IPU-induced catA activity was detected in both P. putida and expressed E. coli. The supernatant of both P. putida and expressed E. coli had a significant influence on weed growth. The study clearly exhibited that P. putida and expressed E. coli were capable of metabolizing IPU influentially and thus could be utilized for bioremediation and biodegradation technology development.

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Identification, Characterization and Genetic Improvement of Bacteriocin Producing Lactic Acid Bacteria

Cited by 9 publications

References 20 publications

Alterations in the expression of certain midgut genes of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and midgut histopathology in response to Bacillus thuringiensis Cry1C toxin

Alterations in the expression of certain midgut genes of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and midgut histopathology in response to Bacillus thuringiensis Cry1C toxin

Draft genome of Raoultella planticola, a high lead resistance bacterium from industrial wastewater

Biodegradation of isoproturon by Escherichia coli expressing a Pseudomonas putida catechol 1,2-dioxygenase gene

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