Identification, characterization and functional analysis of grape (Vitis vinifera L.) mitochondrial transcription termination factor (mTERF) genes in responding to biotic stress and exogenous phytohormone

Abstract

Background Mitochondrial transcription termination factor (mTERF) is a large gene family which plays a significant role during plant growth under various environmental stresses. However, knowledge of mTERF genes in grapevine (Vitis L.) is limited. Results In this research, a comprehensive analysis of grape mTERF (VvmTERF) genes, including chromosome locations, phylogeny, protein motifs, gene structures, gene duplications, synteny analysis and expression profiles, was conducted. As a result, a total of 25 mTERF genes were identified from the grape genome, which are distributed on 13 chromosomes with diverse densities and segmental duplication events. The grape mTERF gene family is classified into nine clades based on phylogenetic analysis and structural characteristics. These VvmTERF genes showed differential expression patterns in response to multiple phytohormone treatments and biotic stresses, including treatments with abscisic acid and methyl jasmonate, and inoculation of Plasmopara viticola and Erysiphe necator. Conclusions These research findings, as the first of its kind in grapevine, will provide useful information for future development of new stress tolerant grape cultivars through genetic manipulation of VvmTERF genes.

Characterization and phylogenetic analysis of the complete mitochondrial genome sequence of Diospyros oleifera, the first representative from the family Ebenaceae

“…The number and type of RNA-editing sites differed among the mt genomes of D. oleifera and other species. Like with most angiosperms [73,76], ribosomal proteins (except rps4) and ATPase subunits (except atp6) had a relatively small number of RNA-editing-derived substitutions (1-12 sites), while the transcripts of NADH dehydrogenase subunits and cytochrome c biogenesis genes were significantly edited (11-36 sites; Figure 4), and ccmFn and ccmB had the most RNA-editing sites predicted (36,35).…”

“…Due to the advancement of sequence technology, long reads, used for de novo assembly of organelle genomes without the need for organelle DNA isolation, could be easily generated from high throughput sequencing. The well-established methodology is quite efficient and well accepted in the scientific community [1,31,32,33,34,35,36,37,38,39,40,41,42].…”

Identification, Characterization, and Expression Profile Analysis of the mTERF Gene Family and Its Role in the Response to Abiotic Stress in Barley (Hordeum vulgare L.)

“…Homologous analysis might provide information on the role of HvmTERFs. However, to ascertain HvmTERF function still requires further detailed and extensive experimental work (Yin et al, 2021).…”

“…By contrast, the mTERF gene family has expanded to approximately 30 members during the evolutionary process of land plants (Quesada, 2016;Leister and Kleine, 2020). For example, there are 35 mTERFs in Arabidopsis thaliana, 33 in rice (Oryza sativa) (Kleine, 2012), 31 in maize (Zea mays) (Zhao et al, 2014), 25 in grape (Vitis vinifera) (Yin et al, 2021), and 35 in Capsicum annuum (Tang et al, 2019). The substantial expansion in the number of mTERF genes was accompanied by their increased involvement in diverse RNA metabolism processes, with the majority being involved in rRNA maturation and intron splicing in organelles (Méteignier et al, 2020).…”

Comparative Transcriptome Profiling Analysis Reveals the Adaptive Molecular Mechanism of Yellow-Green Leaf in Rosa beggeriana ‘Aurea’

“…AtGATL12 plays an important role in the chloroplast development in Arabidopsis , the deletion of AtGATL12 gene causes yellow flowers in seedlings ( Su et al, 2011 ). Mitochondrial transcription termination factors ( mTERF protein family) are important regulators of mitochondrial gene transcription, which can regulate the expression of organelle genes at different levels ( Yin et al, 2021 ). In Arabidopsis , the mutant of mTERF9 gene with T-DNA insertion showed obvious yellowing color leaf phenotype, which demonstrate that mTERF9 may be involved in the regulation of chloroplast development in Arabidopsis ( Meteignier et al, 2021 ).…”