Identification, Characterization and Expression Profiling of Stress-Related Genes in Easter Lily (Lilium formolongi)

Abstract

Biotic and abiotic stresses are the major causes of crop loss in lily worldwide. In this study, we retrieved 12 defense-related expressed sequence tags (ESTs) from the NCBI database and cloned, characterized, and established seven of these genes as stress-induced genes in Lilium formolongi. Using rapid amplification of cDNA ends PCR (RACE-PCR), we successfully cloned seven full-length mRNA sequences from L. formolongi line Sinnapal lily. Based on the presence of highly conserved characteristic domains and phylogenetic analysis using reference protein sequences, we provided new nomenclature for the seven nucleotide and protein sequences and submitted them to GenBank. The real-time quantitative PCR (qPCR) relative expression analysis of these seven genes, including LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, LfCyt-b5, and LfRab, demonstrated that they were differentially expressed in all organs examined, possibly indicating functional redundancy. We also investigated the qPCR relative expression levels under two biotic and four abiotic stress conditions. All seven genes were induced by Botrytis cinerea treatment, and all genes except LfHsp70-3 and LfHsp90 were induced by Botrytis elliptica treatment; these genes might be associated with disease tolerance mechanisms in L. formolongi. In addition, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfUb, and LfCyt-b5 were induced by heat treatment, LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, and LfCyt-b5 were induced by cold treatment, and LfHsp70-1, LfHsp70-2, LfHsp70-3, LfHsp90, LfCy-b5, and LfRab were induced by drought and salt stress, indicating their likely association with tolerance to these stress conditions. The stress-induced candidate genes identified in this study provide a basis for further functional analysis and the development of stress-resistant L. formolongi cultivars.

Intracellular Vesicle Trafficking Genes, RabC-GTP, Are Highly Expressed Under Salinity and Rapid Dehydration but Down-Regulated by Drought in Leaves of Chickpea (Cicer arietinum L.)

“…SsRab2 was found to share 90% similarity to Rab2 proteins found in rice, maize, Arabidopsis, Lotus japonicus and soybean (O’Mahony and Oliver, 1999). Since that time, links to various stresses have been established for genes encoding Rab proteins in numerous plants, and especially in species with high abiotic stress-tolerance such as Lilium formolongi – LfRabB (Howlader et al, 2017), poplar – PtRabE1b (Zhang et al, 2018), and Mesembryanthemum crystallinum – McRab5b (= McRabF ) (Bolte et al, 2000). Interestingly, many plant species were studied for RabG genes and their corresponding proteins including the halophyte species, Aeluropus lagopoides – AlRab7 (= AlRabG ) (Rajan et al, 2015) and food grain crop, Pennisetum glaucum – PgRab7 (= PgRabG ) (Agarwal et al, 2008), as well as more stress susceptible crops such as rice, Oryza sativa – OsRab7 (= OsRabG ) (Nahm et al, 2003) and peanut, Arachis hypogaea – AhRabG (Sui et al, 2017), and the model species A. thaliana – AtRab7 (= AtRabG ) (Mazel et al, 2004).…”

Transcriptome Analysis by RNA–Seq Reveals Genes Related to Plant Height in Two Sets of Parent-hybrid Combinations in Easter lily (Lilium longiflorum)

“…Lily is a highly popular cut flower in Korea. Annual production of lilies in Korea has an economic value of about US$ 34 million 45,46 . In lily, modern hybrid breeding exploit hybrid vigor of inter-sectional hybridization of Longiflorum, Oriental and Asiatic hybrids 47 .…”

Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with Na+ accumulation in leaves of chickpea (Cicer arietinum L.)

“…RabB (Rab2) members have also been shown to be responsive to abiotic stresses. SsRabB (also SsRab2) transcript levels in the desiccation-tolerant grass Sporobolus stapfianus increased in response to dehydration and then decreased after rehydration [34], while LfRab from the Easter lily, Lilium formolongi was induced by both drought and salt stress [35]. RabC (Rab18), RabD (Rab1) and RabE (Rab8) genes have been less studied in plant stress experiments.…”