Identification, characterization and expression analysis of Anopheles stephensi double peroxidase

Choudhury, Tania Pal; Gupta, Lalita; Kumar, Sanjeev

doi:10.1016/j.actatropica.2018.10.008

Cited by 5 publications

(8 citation statements)

References 68 publications

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“…For colony propagation, 4-to 5-day-old females were fed on anesthetized mice and their eggs were collected in moist conditions. The hatched larvae were floated in water to continue the life cycle and fed on a 1:1 mixture of dog food (Pet Lover's crunch milk biscuit, India) and fish food (Gold Tokyo, India) (Gupta et al, 2017;Choudhury et al, 2019;Kakani et al, 2019).…”

Section: Rearing Of Mosquitoesmentioning

confidence: 99%

“…The best matching An. stephensi contig 7145 (SuperContig KB664566 and Ensembl identifier ASTE003848) was analyzed using Augustus software to identify the full-length putative AsHPX2 gene as before (Stanke et al, 2008;Choudhury et al, 2019;Kakani et al, 2019). AsHPX2 and AgHPX2 gene sequences were aligned to design gene-specific primers.…”

Section: Retrieval Of Heme Peroxidase Ashpx2mentioning

confidence: 99%

“…Our analyses of Anopheles stephensi genome revealed the presence of 18 heme-peroxidases that participate in important biological functions (Kajla et al, 2016b;Choudhury et al, 2019;Kakani et al, 2019). In this study, we characterized An.…”

Section: Introductionmentioning

confidence: 97%

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Heme-Peroxidase 2, a Peroxinectin-Like Gene, Regulates Bacterial Homeostasis in Anopheles stephensi Midgut

2020

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The dynamic nature of mosquito gut microbiome is associated with different stages of development and feeding behaviors. Therefore, mosquito gut harbors a wide range of endogenous microbes that promote numerous life processes such as, nutrition, reproduction and immunity. In addition, gut microbiota also play an important role in the regulation of Plasmodium (malaria parasite) development. Thus, understanding the mechanism of microbial homeostasis in mosquito gut might be one of the strategies to manipulate malaria parasite development. In the present study, we characterized a 692 amino acids long secreted midgut heme-peroxidase 2 (AsHPX2) in Anopheles stephensi, the major Indian malaria vector. The presence of putative integrin binding motifs, LDV (Leu-Asp-Val), indicated its peroxinectin-like nature. Our phylogenetic analysis revealed that AsHPX2 is a Culicinae lineage-specific gene. RNA interference (RNAi)-mediated silencing of AsHPX2 gene significantly enhanced the growth of midgut bacteria in sugar-fed mosquitoes against sham-treated controls. Interestingly, bloodfeeding drastically reduced AsHPX2 gene expression and enhanced the growth of midgut bacteria. These results revealed a negative correlation between the expression of AsHPX2 gene and gut bacterial growth. We proposed that AsHPX2, being a mosquitospecific gene, might serve as a "potent target" to manipulate midgut microbiota and vector competence.

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Section: Rearing Of Mosquitoesmentioning

confidence: 99%

Section: Retrieval Of Heme Peroxidase Ashpx2mentioning

confidence: 99%

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Heme-Peroxidase 2, a Peroxinectin-Like Gene, Regulates Bacterial Homeostasis in Anopheles stephensi Midgut

2020

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“…DBLOX is a unique enzyme with two duplicated heme peroxidase domains that is present in insects, but not in vertebrates. The first domain has the predicted substrate binding sites but lacks the functional residues present in catalytically active enzymes and has two integrin-binding motifs, typical of peroxinectins (17). In contrast, the second domain has all the features of a functional heme peroxidase and one integrin-binding motif (17).…”

Section: Antibodies To Recombinant Dblox Detected a Single Band Undermentioning

confidence: 99%

“…The first domain has the predicted substrate binding sites but lacks the functional residues present in catalytically active enzymes and has two integrin-binding motifs, typical of peroxinectins (17). In contrast, the second domain has all the features of a functional heme peroxidase and one integrin-binding motif (17). DBLOX is essential for LXA 4 synthesis, but the biochemical mechanism of lipoxygenase-independent lipoxin synthesis in insects, and the potential involvement of other enzymes besides DBLOX remain to be explored.…”

Section: Antibodies To Recombinant Dblox Detected a Single Band Undermentioning

confidence: 99%

“Proliferation of DBLOX Peroxidase-Expressing Oenocytes Maintains Innate Immune Memory in Primed Mosquitoes”

Gomes

Tyner²,

Barletta³

et al. 2020

Preprint

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Immune priming in Anopheles gambiae mosquitoes following infection with Plasmodium parasites is mediated by the systemic release of a hemocyte differentiation factor (HDF), a complex of lipoxin A4 bound to Evokin, a lipid carrier. HDF increases the proportion of circulating granulocytes and enhances mosquito cellular immunity. We found that Evokin is constitutively produced by hemocytes and fat-body cells, but expression increases in response to infection. Insects synthesize lipoxins, but lack lipoxygenases. Here, we show that the Double Peroxidase (DBLOX) enzyme, present in insects but not in vertebrates, is essential for HDF synthesis. DBLOX is highly expressed in oenocytes in the fat body tissue, and these cells proliferate in response to Plasmodium challenge. We provide direct evidence that modifications mediated by the histone acetyltransferase AgTip60 (AGAP01539) are essential for sustained oenocyte proliferation, HDF synthesis and immune priming. We propose that oenocytes function as a population of “memory” cells that continuously release lipoxin to orchestrate and maintain a broad, systemic and long-lasting state of enhanced immune surveillance.

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BmPxt1 mediated immune response by regulating PGE2 in silkworm, Bombyx mori

Shi,

Cheng,

Zhou

et al. 2023

Pesticide Biochemistry and Physiology

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Identification, characterization and expression analysis of Anopheles stephensi double peroxidase

Cited by 5 publications

References 68 publications

Heme-Peroxidase 2, a Peroxinectin-Like Gene, Regulates Bacterial Homeostasis in Anopheles stephensi Midgut

Heme-Peroxidase 2, a Peroxinectin-Like Gene, Regulates Bacterial Homeostasis in Anopheles stephensi Midgut

“Proliferation of DBLOX Peroxidase-Expressing Oenocytes Maintains Innate Immune Memory in Primed Mosquitoes”

BmPxt1 mediated immune response by regulating PGE2 in silkworm, Bombyx mori

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