Identification, Characterization, and Evaluation of Polymorphic Microsatellite Markers for Parentage Assignment in Pinctada fucata

Abstract

Parentage analysis is a useful tool for establishing pedigree relationships in selective breeding. In this study, 31 microsatellite markers were tested, and 13 loci were subsequently used to identify parenthood in Pinctada fucata. According to simulation analysis, the power of nine loci to exclude false parents was 99.94%, and that of 10 loci was 99.95%. Moreover, using 10 loci and known parental and filial information, the cumulative assignment success rate to one true parental pair was as high as 99%. Of the 120 progeny tested, 93.3% were exclusively assigned to their parental pairs in mixed pedigrees. These results suggested that these microsatellite markers can be used for rapid and effective parentage assignment and contribute to the selective breeding of P. fucata.

Parentage Determination of Chinese Hooksnout Carp (Opsariichthys bidens) Based on Microsatellite DNA Markers

“…Using microsatellite for parentage determination could overcome the limitations of traditional physical tags. Due to their high polymorphism and codominance, microsatellite markers have been widely utilized in parentage determination of aquaculture species, such as Siniperca chuatsi, Siniperca scherzeri, Odontobutis potamophlia, Scylla paramamosain, Fenneropenaeus chinensis, Penaeus monodon, Exopalaemon carinicauda, and P. fucata [14,19,21,[34][35][36][37][38].…”

“…Fortunately, due to the rapid advancements in sequencing technologies [13], highly polymorphic microsatellite markers have been developed for numerous aquaculture species, such as Pinctada fucata, pearl oyster (Pinctada maxima), and black amur bream (Megalobrama terminalis) [14][15][16]. Because of its high density, genome-wide distribution, and codominance, microsatellite has been widely used in the survey of genetic diversity and population structure [17,18].…”