Identification, Characterization and Evaluation of Multifaceted Traits of Plant Growth Promoting Rhizobacteria from Soil for Sustainable Approach to Agriculture

Singh, Tara; Sahai, Vaibhav; Goyal, D.P.; Prasad, Mrinalini; Yadav, Akhilesh; Shrivastav, Preksha; Ali, Akbar; Dantu, Prem Kumar

doi:10.1007/s00284-020-02165-2

Cited by 22 publications

(14 citation statements)

References 40 publications

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“…Accordingly, 79.41% rhizobacterial isolates were shown to be positive in phosphate solubilization, 67.75% ammonia production, 58.82% HCN production, 55.88% siderophore production and 67.75% IAA production. This was confirmatory to earlier reports on rhizobacteria and endophytes [ 84 , 85 , 86 ]. IAA is recorded as one of the most plentiful and secreted by different genera of bacteria, such as Rhizobium , Azospirillum , Bacillus and Pseudomonas [ 87 , 88 ].…”

Section: Discussionsupporting

confidence: 93%

Screening and Biocontrol Potential of Rhizobacteria Native to Gangetic Plains and Hilly Regions to Induce Systemic Resistance and Promote Plant Growth in Chilli against Bacterial Wilt Disease

Kashyap

Manzar

Rajawat

et al. 2021

Plants

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Plant growth-promoting rhizobacteria (PGPR) is a microbial population found in the rhizosphere of plants that can stimulate plant development and restrict the growth of plant diseases directly or indirectly. In this study, 90 rhizospheric soil samples from five agro climatic zones of chilli (Capsicum annuum L.) were collected and rhizobacteria were isolated, screened, and characterized at morphological, biochemical, and molecular levels. In total, 38% of rhizobacteria exhibited the antagonistic capacity to suppress Ralstonia solanacearum growth and showed PGPR activities such as indole acetic acid production by 67.64% from total screened rhizobacteria isolates, phosphorus solubilization by 79.41%, ammonia by 67.75%, HCN by 58.82%, and siderophore by 55.88%. We performed a principal component analysis depicting correlation and significance among plant growth-promoting activities, growth parameters of chilli, and rhizobacterial strains. Plant inoculation studies indicated a significant increase in growth parameters, and PDS1 strain showed maximum 71.11% biocontrol efficiency against wilt disease. The best five rhizobacterial isolates demonstrating both plant growth-promotion traits and biocontrol potential were characterized and identified as PDS1—Pseudomonas fluorescens (MN368159), BDS1—Bacillus subtilis (MN395039), UK4—Bacillus cereus (MT491099), UK2—Bacillus amyloliquefaciens (MT491100), and KA9—Bacillus subtilis (MT491101). These rhizobacteria have the potential natural elicitors to be used as biopesticides and biofertilizers to improve crop health while warding off soil-borne pathogens. The chilli cv. Pusa Jwala treated with Bacillus subtilis KA9 and Pseudomonas fluorescens PDS1 showed enhancement in the defensive enzymes PO, PPO, SOD, and PAL activities in chilli leaf and root tissues, which collectively contributed to induced resistance in chilli plants against Ralstonia solanacearum. The induction of these defense enzymes was found higher in leave tissues (PO—4.87-fold, PP0—9.30-fold, SOD—9.49-fold, and PAL—1.04-fold, respectively) in comparison to roots tissue at 48 h after pathogen inoculation. The findings support the view that plant growth-promoting rhizobacteria boost defense-related enzymes and limit pathogen growth in chilli plants, respectively, hence managing the chilli bacterial wilt.

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Section: Discussionsupporting

confidence: 93%

Screening and Biocontrol Potential of Rhizobacteria Native to Gangetic Plains and Hilly Regions to Induce Systemic Resistance and Promote Plant Growth in Chilli against Bacterial Wilt Disease

Kashyap

Manzar

Rajawat

et al. 2021

Plants

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“…All of these are reported to show PGP activities. For example, Pseudomonas and Acinetobacter are well studied for their biofilm properties 38 , and siderophore production 39 , Enterobacter cloacae (most of Enterobacter in present study were annotated as Enterobacter cloacae in rhizosphere) are associated with heavy metal and salinity stress removal 40 , 41 , and Bacillus are reported to possess several beneficial activities including solubilization of phosphate, nitrogen fixation and siderophore production 42 . UBA2421 , a genus observed with > 3% abundance in all samples, is a yet uncharacterised organism belonging to Planctomycetota phylum.…”

Section: Discussionmentioning

confidence: 80%

Characterizing rhizosphere microbiota of peanut (Arachis hypogaea L.) from pre-sowing to post-harvest of crop under field conditions

Hinsu

Panchal

Pandit

et al. 2021

Sci Rep

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The rhizosphere, a narrow zone of soil near plant roots, is a hot spot for microbial activity. Rhizosphere microbiota directly or indirectly benefit plants by supplementing nutrients, producing beneficial chemicals, or suppressing pathogens. Plants attract and modulate bacteria within the rhizosphere by releasing exudates. Plants also tend to select the rhizosphere microbiota based on their needs; a phenomenon termed as “rhizosphere effect”. In this study, we characterized the rhizosphere microbiota of peanut plants across the crop development cycle from pre-sowing of seeds to post-harvest of crop under field conditions. The rhizosphere and bulk soil samples from different crop developmental stages were also compared. The composition of bulk soil microbiota resembled microbiota of pre-sowing and post-harvest soil and was markedly different from rhizosphere soil samples. Rhizosphere samples were enriched with multiple organisms mostly from the Proteobacteria, Firmicutes and Bacteroidota phyla. Differences in diversity were observed among the rhizosphere samples but not in bulk soil across different crop development stages. Pseudomonas_M indica was highly enriched during the germination of seeds. Furthermore, Plant Growth Promoting (PGP) bacteria like Bacillus were enriched during the middle stages of crop development but there was a decline in PGP organisms in the matured crop stage. We also observed a significant association of pH and Electrical Conductivity (EC) with the profiles of microbial community. Overall, this study portrayed the changes in rhizosphere microbiota of peanut during different developmental stages of crop and may help to design stage specific bio-strategies such as bio-fertilizer to improve crop yield.

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“…Treatment of these PGPR strains significantly promoted cucumber growth. In addition, the B. arboris CSRS12 isolated by Singh et al (2020) promoted mung bean (Vigna radiata) lateral root growth by solubilizing phosphate and siderophores. This is different from the underlying mechanism that B. arboris J211 promotes tobacco plant growth through high-yield IAA observed in this study.…”

Section: Discussionmentioning

confidence: 99%

Nematicidal Activity of Burkholderia arboris J211 Against Meloidogyne incognita on Tobacco

Zhang

Ouyang

et al. 2022

Front. Microbiol.

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Root-knot nematode (Meloidogyne incognita) is the most widespread nematode affecting Solanaceae crops. Due to the lack of effective measures to control this nematode, its management can be achieved, using biocontrol agents. This study investigated in vitro efficacy of the antagonistic bacterial strain J211 isolated from tobacco rhizosphere soil against M. incognita, and further assessed its role in controlling nematodes, both in pot and field trials. Phylogenetic analysis of the 16S rRNA gene sequence of strain J211 assigned to Burkholderia arboris. Culture filtrates B. arboris J211 exhibited anematicidal activity against the second-stage juveniles (J2s) of M. incognita, with a 96.6% mortality after 24 h exposure. Inoculation of J211 in tobacco roots significantly reduced the root galling caused by M. incognita, both in pot and field trials. Meanwhile, plant growth-promoting (PGP) traits results showed that J211 had outstanding IAA-producing activity, and the IAA production reached 66.60 mg L−1. In the field study, B. arboris J211 also promoted tobacco growth and increase flue-cured tobacco yield by 8.7–24.3%. Overall, B. arboris J211 as a high-yielding IAA nematicidal strain effectively controlled M. incognita and improved tobacco yield making it a promising alternative bionematocide.

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Identification, Characterization and Evaluation of Multifaceted Traits of Plant Growth Promoting Rhizobacteria from Soil for Sustainable Approach to Agriculture

Cited by 22 publications

References 40 publications

Screening and Biocontrol Potential of Rhizobacteria Native to Gangetic Plains and Hilly Regions to Induce Systemic Resistance and Promote Plant Growth in Chilli against Bacterial Wilt Disease

Screening and Biocontrol Potential of Rhizobacteria Native to Gangetic Plains and Hilly Regions to Induce Systemic Resistance and Promote Plant Growth in Chilli against Bacterial Wilt Disease

Characterizing rhizosphere microbiota of peanut (Arachis hypogaea L.) from pre-sowing to post-harvest of crop under field conditions

Nematicidal Activity of Burkholderia arboris J211 Against Meloidogyne incognita on Tobacco

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