Identification, Characterization, and Antioxidant Potential of Bifidobacterium longum subsp. longum Strains Isolated From Feces of Healthy Infants

Zhao, Li; Wang, Song; Dong, Jiahuan; Shi, Jialu; Guan, Jiaqi; Liu, Deyu; Liu, Fei; Li, Bailiang; Gui-cheng, Huo

doi:10.3389/fmicb.2021.756519

Cited by 17 publications

(20 citation statements)

References 72 publications

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“…Higher ( P < 0.01) levels of hydrophobicity were also detected for B. longum BH15 (22.78%), B. breve BH5 (22.35%), and B. breve BH4 (17.30%) strains compared to the other strains including L. casei ATCC 393 in n ‐hexane solution. Hydrophobicity levels of these strains were close to those reported by Zhao et al 7 . who found hydrophobicity varying from 21.70% to 69.67% for Bifidobacterium spp.…”

Section: Resultssupporting

confidence: 89%

Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

Güler,

Çetin,

Albayrak

et al. 2024

J Sci Food Agric

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BACKGROUNDNeonatal feces are one of the most important sources for probiotic isolation. The purpose of this study was the isolation and identification of Bifidobacterium spp. from neonatal feces and the evaluation of in vitro probiotic properties of strains including safety tests.RESULTSA total of 40 isolates were obtained from 14 healthy newborns' feces in Erzurum province, Türkiye. By their rep‐PCR patterns and 16S rRNA gene sequences, isolates were identified as 26 Bifidobacterium breve and 14 Bifidobacterium longum. Fifteen of the isolates tolerated bile salts and showed high resistance to simulated gastric juice. Isolates exhibited varying rates of auto‐aggregation and hydrophobicity. In addition, most of the isolates displayed antibacterial activity against Escherichia coli O157:H7, Staphylococcus aureus ATCC 29213, Salmonella Typhimurium RSHMB 95091, and Pseudomonas aeruginosa ATCC 9027. However, only one strain showed bile salt hydrolase activity and two strains showed the ability to produce H2O2. Bifidobacterium strains were generally sensitive to the tested antibiotics and lacked kanamycin, gentamicin, and streptomycin resistance genes, and hemolytic and DNAse activities. On the other hand, it was determined that five strains had various virulence genes including gelE, esp, efaAfs, hyl, and ace.CONCLUSIONResults of the present study suggested that B. longum BH28, B. breve BH4 and B. breve BH5 strains have the potential as probiotic candidates for further studies. © 2024 Society of Chemical Industry.

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Section: Resultssupporting

confidence: 89%

Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

Güler,

Çetin,

Albayrak

et al. 2024

J Sci Food Agric

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“…The cell-free culture supernatant produced by Bifidobacterium longum subsp. exhibited strong scavenging activity on DPPH and hydroxyl radicals, which was consistent with our study 41. This manifested that BFL exhibited a strong radical scavenging and anti-oxidant effect.…”

supporting

confidence: 92%

The pivotal role of Bifida Ferment Lysate on reinforcing the skin barrier function and maintaining homeostasis of skin defenses in vitro

Wang,

Yan,

et al. 2023

J of Cosmetic Dermatology

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BackgroundThe semiactive or inactive probiotics or their extracts used in dermatology have interesting properties to ameliorate signs of irritated skin and enhance the skin barrier. Bifidobacterium, as the most common probiotics, which has been found to be effective in reducing acne and improving the skin barrier function of atopic dermatitis. Bifida Ferment Lysate (BFL) can be obtained from Bifidobacterium by fermentation and extraction.PurposeIn this study, we investigated the effect of a topically used BFL on the skin using in vitro evaluation methods.ResultsThe results showed that upregulation of skin physical barrier gene (FLG, LOR, IVL, TGM1, and AQP3) and antimicrobial peptide gene (CAMP and hBD‐2) in HaCaT cells by BFL might be responsible for skin barrier resistance. In addition, BFL had strong antioxidant properties representing a dose‐dependent increasing of the scavenging capacity of DPPH, ABTS, hydroxyl, and superoxide radicals. BFL treatment also fundamentally inhibited the intracellular ROS and MDA production and improved the activities of antioxidant enzymes (CAT and GSH‐Px) in H2O2‐stimulated HaCaT cells. As a good immunomodulatory factor, BFL efficiently decreased the secretion of IL‐8 and TNF‐α cytokines, and COX‐2 mRNA expression in LPS‐induced THP‐1 macrophages.ConclusionBFL can strengthen the skin barrier function and stimulate skin barrier resistance, to reinforce the skin against oxidative stress and inflammatory stimuli.

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“…On the other hand, the increased abundance of Bifidobacterium longum subsp. Longum in the IBD samples may be attributed to host-responsive mechanisms against gut inflammation since this species has been demonstrated to possess strong antioxidant capacity [ 26 ], attenuate intestinal injury [ 27 ], and generally protect against IBD [ 28 ]. Alterations in the bacteria population is also linked with patients’ response to treatment as demonstrated by studies such as Dovrolis et al [ 29 ], which reported that Infliximab treatment has a notable impact on both the gut microbial composition and the inflamed tissue transcriptome in IBD patients [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

The gut metagenomics and metabolomics signature in patients with inflammatory bowel disease

Ocansey

Hang

et al. 2022

Gut Pathog

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Inflammatory bowel disease (IBD), a chronic gut immune dysregulation and dysbiosis condition is rapidly increasing in global incidence. Regardless, there is a lack of ideal diagnostic markers, while conventional treatment provides scarce desired results, thus, the exploration for better options. Changes in the gut microbial composition and metabolites either lead to or are caused by the immune dysregulation that characterizes IBD. This study examined the fecal metagenomics and metabolomic changes in IBD patients. A total of 30 fecal samples were collected from 15 IBD patients and 15 healthy controls for 16S rDNA gene sequencing and UHPLC/Q-TOF-MS detection of metabolomics. Results showed that there was a severe perturbation of gut bacteria community composition, diversity, metabolites, and associated functions and metabolic pathways in IBD. This included a significantly decreased abundance of Bacteroidetes and Firmicutes, increased disease-associated phyla such as Proteobacteria and Actinobacteria, and increased Escherichiacoli and Klebsiellapneumoniae in IBD. A total of 3146 metabolites were detected out of which 135 were differentially expressed between IBD and controls. Metabolites with high sensitivity and specificity in differentiating IBD from healthy individuals included 6,7,4′-trihydroxyisoflavone and thyroxine 4′-o-.beta.-d-glucuronide (AUC = 0.92), normorphine and salvinorin a (AUC = 0.90), and trichostachine (AUC = 0.91). Moreover, the IBD group had significantly affected pathways including primary bile acid biosynthesis, vitamin digestion and absorption, and carbohydrate metabolism. This study reveals that the combined evaluation of metabolites and fecal microbiome can be useful to discriminate between healthy subjects and IBD patients and consequently serve as therapeutic and diagnostic targets.

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Identification, Characterization, and Antioxidant Potential of Bifidobacterium longum subsp. longum Strains Isolated From Feces of Healthy Infants

Cited by 17 publications

References 72 publications

Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

Isolation, identification, and in vitro probiotic characterization of forty novel Bifidobacterium strains from neonatal feces in Erzurum province, Türkiye

The pivotal role of Bifida Ferment Lysate on reinforcing the skin barrier function and maintaining homeostasis of skin defenses in vitro

The gut metagenomics and metabolomics signature in patients with inflammatory bowel disease

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