Identification case of evidence in timber tracing of Pinus radiate, using high-resolution melting (HRM) analysis

Solano, Jaime; Anabalón, Leonardo; Encina, Francisco

doi:10.1016/j.fsigen.2015.09.003

Cited by 6 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HRM analysis is a method that measures the dissociation rates of double-stranded DNA into single-stranded DNA at increasing temperatures. 15 HRM involves accurate precise monitoring of fluorescence changes caused by the release of an intercalating DNA dye from doublestranded DNA during its denaturation caused by increased temperatures. 22 These analyses allowed to determine the genetic homogeneity in the seeds contained in each package of a certain strain.…”

Section: Discussionmentioning

confidence: 99%

“…These molecular methods can detect differences at the DNA level and they offer numerous advantages over the conventional phenotype-based approaches. 14,15 Barcoding is the analysis of specific conserved regions of the genome to identify genetic sequences of different species. The term ''DNA barcode'' for global species identification was first coined by Hebert in 2003.…”

Section: Introductionmentioning

confidence: 99%

“…DNA sequence differences, such as single nucleotide polymorphisms and small insertions and deletions (Indels), can be detected based on the location of a differential peak and the shape of the melting transition curves. 15,24 The ITS2 region barcode marker was chosen because it is widely used for phylogenetic reconstructions at both genus and species levels. 25,26 In addition, the chloroplast ribulose bisphosphate carboxylase large chain gene (rbcL) is another barcode region used in most land plants due to the ease of amplification, the availability of databases 21,[27][28][29] and its potential application for authenticity testing.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cannabis Seeds Authentication by Chloroplast and Nuclear DNA Analysis Coupled with High-Resolution Melting Method for Quality Control Purposes

Anabalón

Solano

Encina‐Montoya

et al. 2022

Cannabis and Cannabinoid Research

Self Cite

View full text Add to dashboard Cite

Background: Cannabis plants and their seed have been used in many cultures as a source of medicine and feeding during history. Today, there is an increasing demand for cannabis seeds for medical use. Moreover, a seed sales market with no legal regulations has also grown. This may pose some issues if a quality control is not set in place. Identification of cannabis strains is important for quality control purposes in a nonregulated growing market and in cases of illegal traffic and medical use. Owing to the high price as a pharmacological drug, commercial products of cannabis plants and seeds for medical users are often subjected to adulterations, either when packing or distributing certified seeds in the market. Materials and Methods: Cannabis commercial seeds and cannabis seeds for medical use were analyzed with high-resolution melting (HRM) analysis using barcoding markers. Humulus lupulus L. plants from a local market were used as outgroup control. DNA barcoding uses specific regions of the genome to identify differences in the genetic sequence of conserved regions such as internal transcribed spacer (ITS) and rbcL. DNA barcoding data can be generated with real-time polymerase chain reaction combined with HRM analysis to distinguish specific conserved DNA regions of closely related species. HRM analysis is the method of choice for rapid analysis of sequence variation. Results: The melting temperature (T m ) of homogeneous packages was consistent with single genotypes. However, packages containing contaminating seeds showed T m differences of 0.2°C on average. Conclusions: An effective, rapid, and low-cost method based on ITS nuclear DNA and on chloroplast rbcL regions for screening and detection of contamination in commercial cannabis seeds was developed and applied for the analysis of different samples. This approach can be used as a quality control tool for cannabis seeds or other plant material.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cannabis Seeds Authentication by Chloroplast and Nuclear DNA Analysis Coupled with High-Resolution Melting Method for Quality Control Purposes

Anabalón

Solano

Encina‐Montoya

et al. 2022

Cannabis and Cannabinoid Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genetic variants with changes in the base composition present differences in their melting temperatures. This was detected by monitoring the fluorescence as the temperature is increased, and the species are differentiated by their characteristic melting curves, visualized by the loss of fluorescence as the DNA duplex melts (Ganopoulos et al, 2013; Solano, Anabalon & Encina, 2016). Distinct nucleotide sequences of conserved DNA region will provide different melting temperatures.…”

Section: Discussionmentioning

confidence: 99%

Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach

Solano

Anabalón

Encina

et al. 2019

PeerJ

Self Cite

View full text Add to dashboard Cite

The genus Nothofagus is the main component of southern South American temperate forests. The 40 Nothofagus species, evergreen and deciduous, and some natural hybrids are spread among Central and Southern Chile, Argentina, New Zealand, Australia, New Guinea and New Caledonia. Nothofagus nervosa, Nothofagus obliqua and Nothofagus dombeyi are potentially very important timber producers due to their high wood quality and relative fast growth; however, indiscriminate logging has degraded vast areas the Chilean forest causing a serious state of deterioration of their genetic resource. The South of Chile has a large area covered by secondary forests of Nothofagus dombeyi. These forests have a high diversity of species, large amount of biomass and high silvicultural potential. This work shows a case of hybrid identification in Nothofagus subgenus in different secondary forests of Chile, using high resolution melting. Unknown samples of Nothofagus subgenus are genetically distinguishable with the ITS region of Nothofagus antarctica, Nothofagus nitida and N. obliqua species. It was not possible to distinguish between unknown samples of Andean versus coastal origin. Melting curves with ITS approach of unknown material are genetically similar, positioned between N. dombeyi and N. antarctica and distant from N. nitida. The unknown samples are genetically very close to Nothofagus dombeyi. This suggests the presence of hybrid individuality between species (N. dombeyi × N. antarctica) with the possibility of introgression towards the gene pool of N. antarctica, producing the deciduous foliage that is both present. The trnL locus has no distinction between the N. dombeyi and N. antarctica species, since a similar melting curve is present and equal Tm (80.00 °C). The trnL locus cannot be genetically distinguished from one unknown sample of Nothofagus to another, as highlighted in this study.

show abstract

DNA-Based Analysis of Plant Material in Forensic Investigations

Robertson¹,

Damaso²,

Meiklejohn³

2021

Handbook of DNA Profiling

View full text Add to dashboard Cite

Identification case of evidence in timber tracing of Pinus radiate, using high-resolution melting (HRM) analysis

Cited by 6 publications

References 5 publications

Cannabis Seeds Authentication by Chloroplast and Nuclear DNA Analysis Coupled with High-Resolution Melting Method for Quality Control Purposes

Cannabis Seeds Authentication by Chloroplast and Nuclear DNA Analysis Coupled with High-Resolution Melting Method for Quality Control Purposes

Hybrid identification in Nothofagus subgenus using high resolution melting with ITS and trnL approach

DNA-Based Analysis of Plant Material in Forensic Investigations

Contact Info

Product

Resources

About