Identification by MALDI-TOF Mass Spectrometry of Mercury-resistant Bacteria Associated with the Rhizosphere of an Apple Orchard

Urquiza, Camille Calzada; Hernández, Iván Arvizu; Medina, Julio Alfonso Cruz; López, Máximo López; Cruz, Jaime Pérez; Zarazúa, Raquel Louvan Rivera; Casas, Zyanya Gloria Mena; Arteaga, Diana Laura Juarez; García, Adriana Anahíd Contreras; Pérez, José Luis Jiménez; Jones, George H.; Campos-Guillén, Juan

doi:10.1080/01490451.2016.1165316

Cited by 8 publications

(3 citation statements)

References 46 publications

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“…Seven strains were selected to be identified by the MALDI-TOF technique. This method had been used by authors [41][42][43] to identify PGPR. Characteristics of organic matter decomposition, siderophore production and species names of these 7 strains were presented in Table 2 after checking the suitability of the identification results with their cell and colony morphological characteristics.…”

Section: Identification Results Of Selected Isolatesmentioning

confidence: 99%

Study on organic matter decomposition and siderophore production of rhizobacteria isolated from black pepper grown in Loc Ninh, Binh Phuoc province, Vietnam

Son¹,

Thanh²,

Anh³

et al. 2023

World J. Adv. Res. Rev.

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This study was carried out determining capability of organic compound decomposition and siderophore synthesis of 39 plant growth promotion rhizobacteria strains isolated from rhizosphere of black pepper (Piper nigrum L.) in a previous study. The ability to degrade organic compounds including proteins, lipids, cellulose and chitin was investigated by agar well diffusion method. The ability to produce siderophore was qualified by liquid CAS method and strains with good results were further investigated by PS-CAS agar plate method. The results showed that the number of bacterial strains capable of degrading protein, cellulose, chitin and lipid were 37, 27, 16 and 6, respectively. Twenty-two strains changed color of CAS agar, of which 6 strains had a band width of 4.5 cm. The seven best strains identified were Proteus mirabilis (LD2), Bacillus megaterium (LT4), B. subtilis (LT2), B. cereus (LD7, LD8, LD16, LT19) by Matrix Assisted Laser Desorption/Ionization-Time of Flight method. These were plant growth-promoting rhizobacteria that had been reported on potential applications in agriculture.

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Section: Identification Results Of Selected Isolatesmentioning

confidence: 99%

Study on organic matter decomposition and siderophore production of rhizobacteria isolated from black pepper grown in Loc Ninh, Binh Phuoc province, Vietnam

Son¹,

Thanh²,

Anh³

et al. 2023

World J. Adv. Res. Rev.

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“…B. simplex has been isolated from the rhizosphere of a variety of plants including wheat, grass, apple and raspberry, and can colonize the roots of several crops enhancing their growth [47,[56][57][58]. B. simplex KBSIF-3 cells were visualized along the length of tomato roots 7 DAI [47].…”

Section: Discussionmentioning

confidence: 99%

Colonization of Pinus radiata D. Don Seedling Roots by Biocontrol Bacteria Erwinia billingiae and Bacillus simplex

Mesanza

Crawford

Coulson

et al. 2019

Forests

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Erwinia billingiae S31R1 and Bacillus simplex S11R41, isolated from the rhizosphere of a healthy tree located in a Pinus radiata D. Don plantation with high presence of fungal pathogens, are antagonists of pine root rot fungi Heterobasidion annosum and Armillaria mellea in vitro and in young trees. For effective biocontrol of these pathogens, the bacteria must stably colonize P. radiata roots following their application. To determine root colonization patterns, the bacteria were transformed with stable plasmids encoding green fluorescent protein (GFP). Transformed E. billingiae was visualized on roots 24 days after soil inoculation by confocal and epifluorescence microscopy, and GFP was detected by ELISA 31 days after inoculation. The presence of E. billingiae microcolonies, in some cases in root intercellular spaces, suggests that bacterial growth was active and localized. Fluorescence of B. simplex S11R41 was visualized on P. radiata roots 31 days after inoculation and its colonization pattern changed from scattered cells to localized microcolonies. Although the populations decreased over time, microcolony formation and localization in specific regions of roots indicated that E. billingiae, normally considered to be an epiphyte, and B. simplex can stably colonize roots of P. radiata.

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“…For example, Martínez-Molina et al [131] used MALDI-TOF MS to identify Rhizobium strains associated with Pisum sativum L. roots, and the method was found to be able to differentiate species that were very closely related genetically and were indistinguishable based on 16S rRNA gene analysis. Urquiza et al [132] used spectrometry to identify bacteria present in the rhizosphere of apple plants grown in soil with a high concentration of mercury, while Avanzi et al [133] assessed the use of MALDI-TOF MS as a microbiota-monitoring method for sites contaminated with copper. In the future, spectrometry and gene sequencing should be used complementarily to assess a larger contingent of microbial diversity.…”

Section: The Primary Methods Of Detection In Use Have Already Become mentioning

confidence: 99%

Microbial Diversity: The Gap between the Estimated and the Known

Vitorino

Bessa

2018

Diversity

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Abstract:The ecological and biotechnological services that microorganisms provide to the planet and human society highlight the need to understand and preserve microbial diversity, which is widely distributed, challenging the severity of certain environments. Cataloging this diversity has also challenged the methods that are currently used to isolate and grow microorganisms, because most of the microbiota that are present in environmental samples have been described as unculturable. Factors such as geographic isolation and host preference also hinder the assessment of microbial diversity. However, prejudiced historical practices, including the prioritization of some species of microorganisms merely because they cause diseases, have long shifted research on fungi and bacteria towards medically relevant microorganisms. Thus, most microorganisms that inhabit the planet are still unknown, as is the potential of these species. Current estimates allow us to predict that the diversity of microorganisms that are present in the various terrestrial ecosystems is enormous. However, understanding this diversity is a challenge for the future of microbial ecology research.

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Identification by MALDI-TOF Mass Spectrometry of Mercury-resistant Bacteria Associated with the Rhizosphere of an Apple Orchard

Cited by 8 publications

References 46 publications

Study on organic matter decomposition and siderophore production of rhizobacteria isolated from black pepper grown in Loc Ninh, Binh Phuoc province, Vietnam

Study on organic matter decomposition and siderophore production of rhizobacteria isolated from black pepper grown in Loc Ninh, Binh Phuoc province, Vietnam

Colonization of Pinus radiata D. Don Seedling Roots by Biocontrol Bacteria Erwinia billingiae and Bacillus simplex

Microbial Diversity: The Gap between the Estimated and the Known

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