Identiﬁcation by HPLC‐PAD‐MS and quantiﬁcation by HPLC‐PAD of phenylethanoid glycosides of five <i>Phlomis</i> species

Kırmızıbekmez, Hasan; Montoro, Paola; Piacente, Sonia; Pìzza, Cosimo; Dönmez, Ali A.; Çalış, İhsan

doi:10.1002/pca.802

Cited by 67 publications

(58 citation statements)

References 14 publications

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“…russeliana and P. kurdica, demonstrated that variations in the content of PhGs can be used for chemotaxonomical study within the same genus [141]. Other studies have also confirmed the utilization of PhGs as taxonomic markers of genus Orobanche [142], Sibthorpia, and Ellisiophyllum (Plantaginaceae) [143].…”

Section: Chemotaxonomymentioning

confidence: 68%

Naturally Occurring Phenylethanoid Glycosides: Potential Leads for New Therapeutics

Fu¹,

Pang²,

Wong

2008

CMC

134

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ABATRACTNatural products have long been regarded as excellent sources for drug discovery given their structure diversity and wide variety of biological activities. Phenylethanoid glycosides are naturally occurring compounds of plant origin and are structurally characterized with a hydroxyphenylethyl moiety to which a glucopyranose is linked through a glycosidic bond. To date several hundred compounds of this type have been isolated from medicinal plants and further pharmacological studies in vitro or in vivo have shown that these compounds possess a broad array of biological activities including antibacterial, antitumor, antiviral, anti-inflammatory, neuro-protective, antioxidant, hepatoprotective, immunomodulatory, and tyrosinase inhibitory actions. Given their extensive activity profile, structure-activity relationships analyses of these compounds have been performed in a number of studies to reveal potential leads for future drug design. This article will summarize the major developments in phenylethanoid glycosides-based research in the past decade. The progresses made in phytochemistry and biological activity studies of these compounds will be reviewed. Particular attention will be given to the novel structures identified to date and the prominent therapeutic values associated with these molecules.

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Section: Chemotaxonomymentioning

confidence: 68%

Naturally Occurring Phenylethanoid Glycosides: Potential Leads for New Therapeutics

Fu¹,

Pang²,

Wong

2008

CMC

134

View full text Add to dashboard Cite

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“…The MS spectrum of compound 3, identified as caffeic acid, reported a quasimolecular ion [M+H] (163 m/z). Compound 1, with the presence of caffeoyl group (163 m/z), was identified as forsythoside B, previously reported in literature in the genus Phlomis [16]. The antioxidant properties of the plant, as well as some of its traditional uses, may be related to this phenylpropanoids.…”

Section: Bioassay-guided Isolation Of Antioxidant Phenylpropanoidsmentioning

confidence: 93%

“…These phenylpropanoids (Fig. 3) have been described in other Phlomis species [3,16,[30][31][32][33][34][35][36], considered as Fig. 3 HPLC-DAD chromatographic profile of subfraction F4-6 at 330 nm and UV-vis spectra of compounds chemotaxonomic markers and reported as radical scavengers [37].…”

Section: Bioassay-guided Isolation Of Antioxidant Phenylpropanoidsmentioning

confidence: 99%

“…Subfraction F4-6 was also analyzed by HPLC-MS, concluding that the antioxidant constituents were phenylpropanoids derivatives. Identification was carried out by comparing retention times and UV-visible and mass spectra of the peaks with a standard verbascoside and literature data [16]. Compound 1 was identified as forsythoside B, compound 2 (the major antioxidant constituent) was identified as verbascoside, and compound 3 as caffeic acid.…”

Section: Bioassay-guided Isolation Of Antioxidant Phenylpropanoidsmentioning

confidence: 99%

“…The column was Prep NovaPak® HRC18 (7,8 × 300 mm) with a C18 Guarda-Pak™ pre-column (Waters). The separations were carried out following a method previously described for phenylpropanoids with slight modifications [16]. The eluents were water with 5 mM acetic acid (A) and acetonitrile (B).…”

Section: Hplc-dad Analysismentioning

confidence: 99%

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Antioxidant Activity and Phenylpropanoids of Phlomis lychnitis L.: A Traditional Herbal Tea

López

Jäger

Akerreta

et al. 2010

Plant Foods Hum Nutr

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Phlomis lychnitis L. (Lamiaceae) is consumed as a traditional herbal tea in Spain. The antioxidant-protective effects as well as its phytoconstituents have never been established. The ability of the methanolic extract to protect cells from oxidative stress was evaluated in rat pheochromocytoma cells (PC12) using hydrogen peroxide as toxic agent. The viability of PC12 cells pre-treated with the methanolic extract of Phlomis lychnitis, determined by the MTT and LDH assays, was significantly improved at the highest dose (p < 0.01). The antioxidant activity was confirmed evaluating the capacity of the plant to scavenge ABTS, DPPH, O(2) . (-) radicals and to inhibit XO. Bioassay guided fractionation led to antioxidant compounds. Qualitative HPLC/DAD/ESI/MS analysis reported phenylpropanoids, verbascoside being the major antioxidant constituent.

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A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats

Yang

Wang

Hao

et al. 2009

Biomedical Chromatography

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A rapid and sensitive method based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the determination of echinacoside in rat plasma was established and fully validated. A single step of liquid-liquid extraction with n-butanol was utilized. Chromatographic separation of the analyte and the internal standard (IS), chlorogenic acid, from the sample matrix was performed using a Capcell-MG C(18) analytical column (100 2.0 mm x 5 microm), with a gradient of acetonitrile and water containing 0.1% acetic acid as the mobile phase. Detection was performed on a triple quadrupole tandem mass spectrometer equipped with electrospray ionization source operated in negative ion selected reaction monitoring mode. The method was linear in the concentration range 10-2500 ng/mL. The deviations of both intra- and inter-day precisions (RSD) were 7.1% and the assay accuracies were within 99.2-106.5%. Echinacoside proved to be stable during sample storage, preparation and analysis when an antioxidant solution was used. The method was successfully applied to a pharmacokinetic study in rats after an intragastric administration of echinacoside (100 mg/kg). With the lower limit of quantification at 10 ng/mL, this method proved to have sufficient selectivity, sensitivity and reproducibility for the pharmacokinetic study of echinacoside.

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Identiﬁcation by HPLC‐PAD‐MS and quantiﬁcation by HPLC‐PAD of phenylethanoid glycosides of five Phlomis species

Cited by 67 publications

References 14 publications

Naturally Occurring Phenylethanoid Glycosides: Potential Leads for New Therapeutics

Naturally Occurring Phenylethanoid Glycosides: Potential Leads for New Therapeutics

Antioxidant Activity and Phenylpropanoids of Phlomis lychnitis L.: A Traditional Herbal Tea

A sensitive and specific liquid chromatography/tandem mass spectrometry method for determination of echinacoside and its pharmacokinetic application in rats

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