Identification by GC-EAD of the two-component trail-following pheromone of Prorhinotermes simplex (Isoptera, Rhinotermitidae, Prorhinotermitinae)

Sillam‐Dussès, David; Kalinová, Blanka; Jiroš, Pavel; Březinová, Anna; Cvačka, Josef; Hanus, Robert; Šobotník, Jan; Bordereau, Christian; Valterová, Irena

doi:10.1016/j.jinsphys.2009.04.007

Cited by 33 publications

(28 citation statements)

References 27 publications

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“…The SNRs of the EAG and the FID were estimated to be 22.8 and 8.6 dB, respectively. The superiority of the EAG as a detector over the conventional FID GC detector for pheromone detection is consistent with the literature 39 .…”

Section: Reliability Of Flow Control Devicesupporting

confidence: 90%

Chopper-modulated gas chromatography electroantennography enabled using high-temperature MEMS flow control device

et al. 2017

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We report the design, fabrication and characterization of a microelectromechanical systems (MEMS) flow control device for gas chromatography (GC) with the capability of sustaining high-temperature environments. We further demonstrate the use of this new device in a novel MEMS chopper-modulated gas chromatography-electroantennography (MEMS-GC-EAG) system to identify specific volatile organic compounds (VOCs) at extremely low concentrations. The device integrates four pneumatically actuated microvalves constructed via thermocompression bonding of the polyimide membrane between two glass substrates with microstructures. The overall size of the device is 32 mm × 32 mm, and it is packaged in a 50 mm × 50 mm aluminum housing that provides access to the fluidic connections and allows thermal control. The characterization reveals that each microvalve in the flow control chip provides an ON to OFF ratio as high as 1000:1. The device can operate reliably for more than 1 million switching cycles at a working temperature of 300°C. Using the MEMS-GC-EAG system, we demonstrate the successful detection of cis-11-hexadecenal with a concentration as low as 1 pg at a demodulation frequency of 2 Hz by using an antenna harvested from the male Helicoverpa Virescens moth. In addition, 1 μg of a green leafy volatile (GLV) is barely detected using the conventional GC-EAG, while MEMS-GC-EAG can readily detect the same amount of GLV, with an improvement in the signal-to-noise ratio (SNR) of~22 times. We expect that the flow control device presented in this report will allow researchers to explore new applications and make new discoveries in entomology and other fields that require high-temperature flow control at the microscale.

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Section: Reliability Of Flow Control Devicesupporting

confidence: 90%

Chopper-modulated gas chromatography electroantennography enabled using high-temperature MEMS flow control device

et al. 2017

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“…Even though we cannot exclude the presence of multiple components, we observed no GC-EAD evidence of other compounds being involved in trail following. This finding is not surprising given that the sternal gland is a universal source of trail pheromones in termites, and dodecatrienol is used as a trail pheromone in all Rhinotermitidae studied (Sillam-Dussès et al, 2009;Bordereau and Pasteels, 2011). The sternal gland of workers does not differ significantly in size from the sternal gland of females, but it differs in having a bilobed shape, as in workers of other rhinotermitids (Ampion and Quennedey, 1981).…”

Section: Discussionmentioning

confidence: 90%

“…Fourth, both trail and sex pheromones most often consist of a single compound, not blends of compounds as in numerous other insects. However, some recent studies demonstrated the presence of minor components in addition to these major compounds (Sillam-Dussès et al, 2009Bordereau et al, 2010;Anani Kotoklo et al, 2010), suggesting that minor active components could have been overlooked in the past.…”

Section: Introductionmentioning

confidence: 99%

Sex Pheromone and Trail Pheromone of the Sand Termite Psammotermes hybostoma

et al. 2011

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Within the complex network of chemical signals used by termites, trail pheromones and sex pheromones are among the best known. Numerous recent papers map the chemical identity and glandular origin of these pheromones in nearly all major isopteran taxa. In this study, we aimed to describe the sex pheromone and the trail pheromone of a poorly known sand termite, Psammotermes hybostoma. We identified (3Z,6Z,8E)-dodeca-3,6,8-trien-1-ol (dodecatrienol) as the sex pheromone released by tergal and sternal glands of female imagos and, at the same time, as the trail pheromone secreted from the sternal gland of workers. We conclude that chemical communication in Psammotermes does not differ from that of most other Rhinotermitidae, such as Reticulitermes, despite the presence of a diterpene as a major component of the trail pheromone of Prorhinotermes to which Psammotermes is presumed to be phylogenetically close. Our findings underline once again the conservative nature of chemical communication in termites, with dodecatrienol being a frequent component of pheromonal signals in trail following and sex attraction and, at the same time, a tight evolutionary relationship between the trail following of working castes and the sex attraction of imagos.

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“…Once food has been found, the termites return to the nest using this exploratory trail while simultaneously laying down a recruitment trail that is far more attractive to foragers (Oloo and Leuthold 1979;Traniello 1982). It is difficult to determine whether exploratory Šobotník et al (2008), Hanus et al (2009Hanus et al ( , 2010, and Sillam-Dussès et al (2009a) and recruitment trails only differ quantitatively, as suggested by the fact that workers lay down a dotted exploratory trail but a continuous recruitment trail, or whether they also differ qualitatively as a result of differences in chemical composition (Affolter and Leuthold 2000). Foraging is often initiated by workers, who are later eventually accompanied by soldiers (e.g., Heidecker and Leuthold 1984;Lys and Leuthold 1991;Reinhard et al 1997a).…”

Section: Foraging and Food Markingmentioning

confidence: 99%

Communication and Social Regulation in Termites

Bagnères

Hanus

2015

Social Recognition in Invertebrates

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Communication and social regulation are among the distinguishing features of termites: they are part of all basic aspects of termite biology, from ontogeny and caste differentiation to social behavior and cooperation. As in other highly social taxa, communication in termites predominantly relies on a complex network of chemical signals, which are complemented by vibration-based signals. In contrast to other social taxa, the role of visual cues is negligible. In this chapter, we review the recent literature on the different components that make up termite communication and social regulation systems by tracing termite evolution and examining the role played by different factors, such as sex and caste, and different behaviors, such as those related to defense, nestmate recognition, egg and brood care, foraging, and nest building, among others. The main characteristics of termites are compared to those of other social insects in the introduction. In the first section, we review the most important researches on termite communication and social regulation that are related to social activities in the basal phylogenetic lineages, and in the Termitidae (higher termites), the most advanced and diversified family. The abundant literature on the best studied genera, Reticulitermes, Coptotermes, and Heterotermes, which are considered for the purposes of this chapter to be intermediate termites, is reviewed and discussed separately in the second section. By using this approach, we seek to describe communication and social regulation systems in basal and primitive termites and illustrate how they have evolved to become more complex in intermediate and higher termites.

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Identification by GC-EAD of the two-component trail-following pheromone of Prorhinotermes simplex (Isoptera, Rhinotermitidae, Prorhinotermitinae)

Cited by 33 publications

References 27 publications

Chopper-modulated gas chromatography electroantennography enabled using high-temperature MEMS flow control device

Chopper-modulated gas chromatography electroantennography enabled using high-temperature MEMS flow control device

Sex Pheromone and Trail Pheromone of the Sand Termite Psammotermes hybostoma

Communication and Social Regulation in Termites

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