Identification and Verification of the Prodigiosin Biosynthetic Gene Cluster (BGC) in Pseudoalteromonas rubra S4059

Wang, Xiyan; Isbrandt, Thomas; Christensen, Emil Ørsted; Melchiorsen, Jette; Larsen, Thomas Ostenfeld; Zhang, Sheng-Da; Gram, Lone

doi:10.1128/spectrum.01171-21

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…Here pigC, pigH, pigI, and pigJ were detected as the prime biosynthetic genes regulated by transcription regulator cueR. As the insertion of transposons or other genomic elements on BGC can significantly alter prodigiosin production in Serratia marcescens [31,32], this above-mentioned finding can be a useful basis of identifying and studying the critical points where mutations or other genomic changes can alter prodigiosin synthesis. However, existence of prodigiosin BGC has also been reported in other bacteria like Vibrio sp., Pseudomonas sp.…”

Section: Plos Onementioning

confidence: 88%

“…However, existence of prodigiosin BGC has also been reported in other bacteria like Vibrio sp., Pseudomonas sp. that exhibit species and strain dependent variation in the number of Open Reading Frame (ORF) [31,33,34]. Absence of virulence and resistance gene in BRL41 genome is another impressive finding as it declares the fitness of this isolate to make use for commercial production of prodigiosin.…”

Section: Plos Onementioning

confidence: 99%

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In silico exploration of Serratia sp. BRL41 genome for detecting prodigiosin Biosynthetic Gene Cluster (BGC) and in vitro antimicrobial activity assessment of secreted prodigiosin

Boby,

Bhuiyan,

Saha

et al. 2023

PLoS ONE

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The raising concern of drug resistance, having substantial impacts on public health, has instigated the search of new natural compounds with substantial medicinal activity. In order to find out a natural solution, the current study has utilized prodigiosin, a linear tripyrrole red pigment, as an active ingredient to control bacterial proliferation and prevent cellular oxidation caused by ROS (Reactive Oxygen Species). A prodigiosin-producing bacterium BRL41 was isolated from the ancient Barhind soil of BCSIR Rajshahi Laboratories, Bangladesh, and its morphological and biochemical characteristics were investigated. Whole genome sequencing data of the isolate revealed its identity as Serratia sp. and conferred the presence of prodigiosin gene cluster in the bacterial genome. “Prodigiosin NRPS”, among the 10 analyzed gene clusters, showed 100% similarity with query sequences where pigC, pigH, pigI, and pigJ were identified as fundamental genes for prodigiosin biosynthesis. Some other prominent clusters for synthesis of ririwpeptides, yersinopine, trichrysobactin were also found in the chromosome of BRL41, whilst the rest displayed less similarity with query sequences. Except some first-generation beta-lactam resistance genes, no virulence and resistance genes were found in the genome of BRL41. Structural illumination of the extracted red pigment by spectrophotometric scanning, Thin-Layer Chromatography (TLC), Fourier Transform Infrared Spectroscopy (FTIR), and change of color at different pH solutions verified the identity of the isolated compound as prodigiosin. Serratia sp. BRL41 attained its maximum productivity 564.74 units/cell at temperature 30˚C and pH 7.5 in two-fold diluted nutrient broth medium. The compound exhibited promising antibacterial activity against Gram-positive and Gram-negative bacteria with MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) values ranged from 3.9 to15.62 μg/mL and 7.81 to 31.25 μg/mL respectively. At concentration 500 μg/mL, except in Salmonella enterica ATCC-10708, prodigiosin significantly diminished biofilm formed by Listeria monocytogens ATCC-3193, Pseudomonas aeruginosa ATCC-9027, Escherichia coli (environmental isolate), Staphylococcus aureus (environmental isolate). Cellular glutathione level (GSH) was elevated upon application of 250 and 500 μg/mL pigment where 125 μg/mL failed to show any free radical scavenging activity. Additionally, release of cellular components in growth media of both Gram-positive and Gram-negative bacteria were facilitated by the extract that might be associated with cell membrane destabilization. Therefore, the overall findings of antimicrobial, antibiofilm and antioxidant activities suggest that in time to come prodigiosin might be a potential natural source to treat various diseases and infections.

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Section: Plos Onementioning

confidence: 88%

Section: Plos Onementioning

confidence: 99%

In silico exploration of Serratia sp. BRL41 genome for detecting prodigiosin Biosynthetic Gene Cluster (BGC) and in vitro antimicrobial activity assessment of secreted prodigiosin

Boby,

Bhuiyan,

Saha

et al. 2023

PLoS ONE

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“…This PG compound passed into the Jones Chromatography column along with mobile phases at ambient temperatures. Elution was performed with 0.1% HPLC-grade formic acid (Merck Limited (Mumbai, India)) as solvent A [ 78 ] and 0.1% HPLC-grade acetonitrile (Merck Limited (Mumbai, India)) as solvent B [ 78 ], with a flow rate of 3 mL/min a detection wavelength of 535 nm [ 52 ], with parameters of 0 to 20 min. As pure PG standards can be cost-prohibitive, the resultant HPLC chromatogram was compared with a reference chromatogram reported by Lee M.A.…”

Section: Methodsmentioning

confidence: 99%

Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications

Arivuselvam,

Dera,

Parween Ali

et al. 2023

Antibiotics

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Prodigiosin pigment has high medicinal value, so exploring this compound is a top priority. This report presents a prodigiosin bioactive compound isolated from Serratia marcescens JSSCPM1, a new strain. The purification process of this compound involves the application of different chromatographic methods, including UV-visible spectroscopy, high-performance liquid chromatography (HPLC), and liquid chromatography–mass spectrometry (LC/MS). Subsequent analysis was performed using nuclear magnetic resonance (NMR) to achieve a deeper understanding of the compound’s structure. Finally, through a comprehensive review of the existing literature, the structural composition of the isolated bioactive compound was found to correspond to that of the well-known compound prodigiosin. The isolated prodigiosin compound was screened for antibacterial activity against both Gram-positive and Gram-negative bacteria. The compound inhibited the growth of Gram-negative bacterial strains compared with Gram-positive bacterial strains. It showed a maximum minimum inhibitory concentration against Escherichia coli NCIM 2065 at a 15.9 ± 0.31 μg/mL concentration. The potential binding capabilities between prodigiosin and the OmpF porin proteins (4GCS, 4GCP, and 4GCQ) were determined using in silico studies, which are generally the primary targets of different antibiotics. Comparative molecular docking analysis indicated that prodigiosin exhibits a good binding affinity toward these selected drug targets.

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“…1, bottom). [38][39][40][41][42] Despite the structural diversity of prodiginine-related natural products, their biosynthesis resorts to clustered genes, encoding for non-ribosomal peptide synthetases (NRPS) of type II. 36,43 Using the synthesis of prodigiosin (1) as representative example, the assembly employs a convergent route with two key precursors, namely the bipyrrole part (4-methoxy-2,2′-bipyrrole-5-carbaldehyde, MBC 3a) and the monopyrrole part (2-methyl-3-amylpyrrole, MAP 4a), which are consolidated in an ATP-catalysed condensation reaction to give the conjugated tripyrrole scaffold of prodigiosin (1) (Fig.…”

Section: Biosynthesis and Condensing Enzymesmentioning

confidence: 99%

New prodigiosin derivatives – chemoenzymatic synthesis and physiological evaluation against cisplatin-resistant cancer cells

Weber,

Leyens,

Berning

et al. 2023

Catal. Sci. Technol.

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Identification and Verification of the Prodigiosin Biosynthetic Gene Cluster (BGC) in Pseudoalteromonas rubra S4059

Cited by 7 publications

References 38 publications

In silico exploration of Serratia sp. BRL41 genome for detecting prodigiosin Biosynthetic Gene Cluster (BGC) and in vitro antimicrobial activity assessment of secreted prodigiosin

In silico exploration of Serratia sp. BRL41 genome for detecting prodigiosin Biosynthetic Gene Cluster (BGC) and in vitro antimicrobial activity assessment of secreted prodigiosin

Isolation, Identification, and Antibacterial Properties of Prodigiosin, a Bioactive Product Produced by a New Serratia marcescens JSSCPM1 Strain: Exploring the Biosynthetic Gene Clusters of Serratia Species for Biological Applications

New prodigiosin derivatives – chemoenzymatic synthesis and physiological evaluation against cisplatin-resistant cancer cells

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