Identification and validation of powdery mildew (Podosphaera xanthii)-resistant loci in recombinant inbred lines of cucumber (Cucumis sativus L.)

Fukino, Nobuko; Yoshioka, Yosuke; Sugiyama, Masao; Sakata, Yoshiteru; Matsumoto, Satoru

doi:10.1007/s11032-013-9867-3

Cited by 67 publications

(59 citation statements)

References 34 publications

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“…Only QTL that explained more than 20 % of the variation are shown. The QTL names given by the authors are maintained (Sakata et al 2006;Liu et al 2008;Zhang et al 2011Zhang et al , 2013Fukino et al 2013;He et al 2013;Pang et al 2013;Yoshioka et al 2014). The main QTL for PM resistance detected by Sakata et al (2006) is located on Chr.…”

Section: Cucumber Has 13 Members In the Mlo Familymentioning

confidence: 99%

Identification of candidate genes required for susceptibility to powdery or downy mildew in cucumber

et al. 2014

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Powdery mildew (PM, caused by Podosphaera fusca) and downy mildew (DM, caused by Pseudoperonospora cubensis) are important diseases of cucumber (Cucumis sativus). Breeding for resistance has been undertaken since the 1940s, but underlying resistance genes have not been functionally analysed yet. The published genome sequence of cucumber catalyses the search for such genes. Genetic studies have indicated that resistances to PM and DM in cucumber are often inherited recessively, which indicates the presence of susceptibility genes (Sgenes). Therefore we analyzed the cucumber genome for homologs of functionally proven S-genes known from other plant species. We identified 13 MLO-like genes in cucumber, three of which cluster in Clade V, the clade that contains all known MLO-like susceptibility genes to powdery mildews in other dicots. The expression of one of these three genes, CsaMLO1, located on chromosome 1, was upregulated after PM inoculation. It co-localizes with a QTL for PM resistance previously identified. Also homologs of the susceptibility genes PMR4 and PMR5 are located at this QTL. The second MLO-like gene from Clade V (CsaMLO8) resides in a recessively inherited major QTL for PM resistance at the bottom of chromosome 5, together with a PMR6-like gene. Two major QTL for DM recessive resistance at the top of chromosome 5 co-localize with CsaDMR6-2, which is homologous to the DMR6 susceptibility gene in Arabidopsis. This study has identified several candidate genes for susceptibility to PM and DM in cucumber that may explain QTL for recessively inherited resistance, reported earlier.

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Section: Cucumber Has 13 Members In the Mlo Familymentioning

confidence: 99%

Identification of candidate genes required for susceptibility to powdery or downy mildew in cucumber

et al. 2014

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“…Powdery mildew (PM hereinafter), mainly caused by Podosphaera fusca [1, 2], is among the most destructive fungal diseases amongst Cucurbitaceae family crops including cucumber, melon, watermelon, pumpkin and squash [3–5]. Because cucumbers are grown year-round in greenhouses or high tunnels, PM finds such sheltered conditions favorable, has had a profound impact on the cucumber industry [6].…”

Section: Introductionmentioning

confidence: 99%

“…Quantitative trait loci (QTLs) associated with PM resistance have been mapped to six cucumber chromosomes [1, 2, 8, 11–13]. Although these findings have provided insights into the genetic control of cucumber PM resistance, the molecular defense mechanisms of host resistance against cucumber PM remain elusive.…”

Section: Introductionmentioning

confidence: 99%

Elucidation of the molecular responses of a cucumber segment substitution line carrying Pm5.1 and its recurrent parent triggered by powdery mildew by comparative transcriptome profiling

Shi

et al. 2017

BMC Genomics

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BackgroundPowdery mildew (PM) is one of the most severe fungal diseases of cucurbits, but the molecular mechanisms underlying PM resistance in cucumber remain elusive. In this study, we developed a PM resistant segment substitution line SSL508-28 that carried a segment on chromosome five representing the Pm5.1 locus from PM resistant donor Jin5-508 using marker-assisted backcrossing of an elite PM susceptible cucumber inbred line D8.ResultsWhole-genome resequencing of SSL508-28, Jin5-508 and D8 was performed to identify the exact boundaries of the breakpoints for this introgression because of the low density of available single sequence repeat markers. This led to the identification of a ~6.8 Mb substituted segment predicted to contain 856 genes. RNA-seq was used to study gene expression differences in PM treated (plants harvested 48 h after inoculation) and untreated (control) SSL508-28 and D8 lines. Exactly 1,248 and 1,325 differentially expressed genes (DEGs) were identified in SSL508-28 and D8, respectively. Of those, 88 DEGs were located in the ~6.8 Mb segment interval. Based on expression data and annotation, we identified 8 potential candidate genes that may participate in PM resistance afforded by Pm5.1, including two tandemly arrayed genes encoding receptor protein kinases, two transcription factors, two genes encoding remorin proteins, one gene encoding a P-type ATPase and one gene encoding a 70 kDa heat shock protein. The transcriptome data also revealed a complex regulatory network for Pm5.1-mediated PM resistance that may involve multiple signal regulators and transducers, cell wall modifications and the salicylic acid signaling pathway.ConclusionThese findings shed light on the cucumber PM defense mechanisms mediated by Pm5.1 and provided valuable information for the fine mapping of Pm5.1 and breeding of cucumber with enhanced resistance to PM.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3438-z) contains supplementary material, which is available to authorized users.

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“…These genetic and genomics resources have greatly facilitated genetic mapping, molecular tagging, and gene cloning in cucumber. However, only a limited number of QTLs have been reported in this crop for disease resistance (Sakata et al 2006;Liu et al 2008;Zhang et al 2011Zhang et al , 2013Fukino et al 2013;He et al 2013), flowering time, fruit size, fruit bitterness, or fruit epidermal features (Kennard and Havey 1995;Dijkhuizen and Staub 2002;Fazio et al 2003;Yuan et al 2008;Cheng et al 2010;Miao et al 2011Miao et al , 2012Qi et al 2013). These studies utilized cultivated or wild cucumbers, but not XIS semi-wild cucumber.…”

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confidence: 99%

Molecular mapping reveals structural rearrangements and quantitative trait loci underlying traits with local adaptation in semi-wild Xishuangbanna cucumber (Cucumis sativus L. var. xishuangbannanesis Qi et Yuan)

Chen

et al. 2014

Theor Appl Genet

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Comparative genetic mapping revealed the origin of Xishuangbanna cucumber through diversification selection after domestication. QTL mapping provided insights into the genetic basis of traits under diversification selection during crop evolution. The Xishuangbanna cucumber, Cucumis sativus L. var. xishuangbannanesis Qi et Yuan (XIS), is a semi-wild landrace from the tropical southwest China with some unique traits that are very useful for cucumber breeding, such as tolerance to low light, large fruit size, heavy fruit weight, and orange flesh color in mature fruits. In this study, using 124 recombinant inbred lines (RILs) derived from the cross of the XIS cucumber with a cultivated cucumber inbred line, we developed a linkage map with 269 microsatellite (or simple sequence repeat) markers which covered 705.9 cM in seven linkage groups. Comparative analysis of orders of common marker loci or marker-anchored draft genome scaffolds among the wild (C. sativus var. hardwickii), semi-wild, and cultivated cucumber genetic maps revealed that the XIS cucumber shares major chromosomal rearrangements in chromosomes 4, 5, and 7 between the wild and cultivated cucumbers suggesting that the XIS cucumber originated through diversifying selection after cucumber domestication. Several XIS-specific minor structural changes were identified in chromosomes 1 and 6. QTL mapping with the 124 RILs in four environments identified 13 QTLs for domestication and diversifying selection-related traits including 2 for first female flowering time (fft1.1, fft6.1), 5 for mature fruit length (fl1.1, fl3.1, fl4.1, fl6.1, and fl7.1), 3 for fruit diameter (fd1.1, fd4.1, and fd6.1), and 3 for fruit weight (fw2.1, fw4.1, and fw6.1). Six of the 12 QTLs were consistently detected in all four environments. Among the 13 QTLs, fft1.1, fl1.1, fl3.1, fl7.1, fd4.1, and fw6.1 were major-effect QTLs for respective traits with each explaining at least 10 % of the observed phenotypic variations. Results from this study provide insights into the cytological and genetic basis of crop evolution leading to the XIS cucumber. The molecular markers associated with the QTLs should be useful in exploring the XIS cucumber genetic resources for cucumber breeding.

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Identification and validation of powdery mildew (Podosphaera xanthii)-resistant loci in recombinant inbred lines of cucumber (Cucumis sativus L.)

Cited by 67 publications

References 34 publications

Identification of candidate genes required for susceptibility to powdery or downy mildew in cucumber

Identification of candidate genes required for susceptibility to powdery or downy mildew in cucumber

Elucidation of the molecular responses of a cucumber segment substitution line carrying Pm5.1 and its recurrent parent triggered by powdery mildew by comparative transcriptome profiling

Molecular mapping reveals structural rearrangements and quantitative trait loci underlying traits with local adaptation in semi-wild Xishuangbanna cucumber (Cucumis sativus L. var. xishuangbannanesis Qi et Yuan)

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