Identification and validation of genetic locus Rfk1 for wheat fertility restoration in the presence of Aegilops kotschyi cytoplasm

Chen, Yanru; Jia, Yulin; Niu, Fuqiang; Wu, Yongfeng; Ye, Jiali; Yang, Xiaohong R.; Zhang, Lingli; Song, Xiyue

doi:10.1007/s00122-020-03738-2

Cited by 7 publications

(6 citation statements)

References 36 publications

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“…Many of the shared DEGs that had those MF annotations encoded pectinesterases (PMEs)/PME inhibitors and pectate lyases. For example, Rfk1 encodes a PME/PME inhibitor protein that is involved in pectin metabolism and regulates fertility restoration in wheat ( Chen et al., 2021 ). Glyma.09G042200 encoded a PME/PME inhibitor enzyme that was strongly down-regulated in W931A at both the UM and BP stages.…”

Section: Resultsmentioning

confidence: 99%

“…PMEs regulate the mechanical and chemical properties of cell walls via methyl esterification of cell wall pectin ( Li et al., 2002 ; Jiang et al., 2005 ; Hepler et al., 2013 ). PMEs play important roles in plant cell wall synthesis and metabolism; abnormal cell wall development affects pollen vitality and can lead to male sterility ( Chen et al., 2021 ). In the wheat line CMS-K, abnormal changes in the pollen intine wall structure and the ATPase activity response are likely important in pollen grain abortion ( Yao et al., 2002 ; Chen et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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Integrated transcriptomic and proteomic analysis of a cytoplasmic male sterility line and associated maintainer line in soybean

Wang

Zhang

et al. 2023

Front. Plant Sci.

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IntroductionHeterosis is a critical phenomenon in crop improvement. Cytoplasmic male sterility (CMS) and Restorer gene (Rf) systems are essential components for heterosis-based breeding. However, the molecular mechanism underlying CMS remains largely unclear in soybean.MethodsWe integrated a morphological investigation with comparative analyses of transcriptomic and proteomic changes in pollen from the CMS line W931A and its maintainer line, W931B, at the uninucleate microspore (UM) and binucleate pollen (BP) stages.ResultsCompared to W931B, which had healthy, oval pollen grains, W931A showed shrunken or degraded pollen grains with an irregularly thickened endothelium and decreased starch accumulation. Transcriptomic comparisons revealed a total of 865 differentially expressed genes (DEGs) in W931A over the two stages. These genes were primarily associated with pentose and glucuronate interconversions, sphingolipid metabolism, and glycerolipid metabolism. Proteomic analysis revealed 343 differentially expressed proteins (DEPs), which were mainly involved in carbon metabolism, glycolysis/gluconeogenesis, and nitrogen metabolism. Consistently, Gene Ontology (GO) biological process terms related to pollen development were enriched among DEGs at the UM and BP stages. Notably, four genes with demonstrated roles in pollen development were differentially expressed, including AGAMOUS-LIKE 104, PROTEIN-TYROSINE-PHOSPHATASE 1, and PHOSPHOLIPASE A2. A total of 53 genes and the corresponding proteins were differentially expressed in W931A at both the UM and BP stages, and many of these were pectinesterases, polygalacturonases, peroxidases, and ATPases.DiscussionThe results of this study suggest that pollen development in W931A is likely regulated through suppression of the identified DEGs and DEPs. These findings increase our understanding of the molecular mechanism underlying CMS in soybean, aiding future research into soybean fertility and promoting the efficient use of heterosis for soybean improvement.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Integrated transcriptomic and proteomic analysis of a cytoplasmic male sterility line and associated maintainer line in soybean

Wang

Zhang

et al. 2023

Front. Plant Sci.

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“…Stamen-specific model contains 47 specifically expressed genes, and seven of them encoding pectinesterase, pectate lyase, and exopolygalacturonase in Bougainvillea . The genes encoding pectinesterase are specifically expressed in stamens in wheat, and are involved in the regulation of male fertility [ 51 ]. The genes encoding exopolygalacturonase in maize are expressed in tissues associated with pollen development [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analyses shed light on floral organ morphogenesis and bract color formation in Bougainvillea

Zhang

Zhou²,

Lin

et al. 2022

BMC Plant Biol

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Background Bougainvillea is a popular ornamental plant with brilliant color and long flowering periods. It is widely distributed in the tropics and subtropics. The primary ornamental part of the plant is its colorful and unusual bracts, rich in the stable pigment betalain. The developmental mechanism of the bracts is not clear, and the pathway of betalain biosynthesis is well characterized in Bougainvillea. Results At the whole-genome level, we found 23,469 protein-coding genes by assembling the RNA-Seq and Iso-Seq data of floral and leaf tissues. Genome evolution analysis revealed that Bougainvillea is related to spinach; the two diverged approximately 52.7 million years ago (MYA). Transcriptome analysis of floral organs revealed that flower development of Bougainvillea was regulated by the ABCE flower development genes; A-class, B-class, and E-class genes exhibited high expression levels in bracts. Eight key genes of the betalain biosynthetic pathway were identified by homologous alignment, all of which were upregulated concurrently with bract development and betalain accumulation during the bract initiation stage of development. We found 47 genes specifically expressed in stamens, including seven highly expressed genes belonging to the pentose and glucuronate interconversion pathways. BgSEP2b, BgSWEET11, and BgRD22 are hub genes and interacted with many transcription factors and genes in the carpel co-expression network. Conclusions We assembled protein-coding genes of Bougainvilea, identified the floral development genes, and constructed the gene co-expression network of petal, stamens, and carpel. Our results provide fundamental information about the mechanism of flower development and pigment accumulation in Bougainvillea, and will facilitate breeding of cultivars with high ornamental value.

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“…However, the restorer Rfk1 (TraesCS1B02G197400LC) located on 1BS chromosome against Ae. Kotschyi cytoplasm is a dominant gene locus derived from LK783, which differs from Rf multi (Chen et al 2021). In this study, Rfd1 was mapped to a physical region of 2.4-Mb containing 19 candidate genes on chromosome 1BS.…”

Section: Discussionmentioning

confidence: 99%

Rfd1, a restorer to theAegilops juvenaliscytoplasm, functions in fertility restoration of wheat cytoplasmic male sterility

Niu

Yang

et al. 2022

Journal of Experimental Botany

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Cytoplasmic male sterility (CMS) is a crucial means for the utilization of heterosis, which is of great significance for improving the yield and quality of hybrids. Currently, fertility restoration has been extensively investigated in crops, but fertility restoration of CMS wheat with Ae. juvenalis cytoplasm is poorly understood. Here, a backcross population BC1F1 derived from a cross between male sterile line Ju706A, its maintainer line 706B, and restorer line LK783, was used to map Rfd1 locus by bulked segregant analysis and wheat 660K SNP genotyping. In which Ju706A displayed complete male sterility, and its fertility can be restored by LK783 with a pair of dominant genes Rfd1Rfd1. The locus was located to 2.4-Mb region on chromosome 1BS by markers AX-174254104 and AX-111201011. Combined with transcriptomic analysis and quantitative real-time PCR assay, TraesCS1B02G197400LC, the most likely candidate gene for Rfd1, encoded a pectinesterase that was localized in the cell wall, and was highly expressed in fertile anthers. The silencing of Rfd1 resulted in decreased fertility and heterogeneous expression of Rfd1 promoted pollen germination and affected the vegetative growth. This implies that Rfd1 is required for anther or pollen development and male fertility in CMS wheat with Ae. juvenalis cytoplasm. Furthermore, a 7 bp deletion in Ju706A was employed to develop a specific marker, Xnwafu1, for molecular marker-assisted selection of restorers. This study provides a new understanding for exploring the fertility restoration mechanism of CMS.

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Identification and validation of genetic locus Rfk1 for wheat fertility restoration in the presence of Aegilops kotschyi cytoplasm

Cited by 7 publications

References 36 publications

Integrated transcriptomic and proteomic analysis of a cytoplasmic male sterility line and associated maintainer line in soybean

Integrated transcriptomic and proteomic analysis of a cytoplasmic male sterility line and associated maintainer line in soybean

Transcriptome analyses shed light on floral organ morphogenesis and bract color formation in Bougainvillea

Rfd1, a restorer to theAegilops juvenaliscytoplasm, functions in fertility restoration of wheat cytoplasmic male sterility

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